Evaluación de los efectos anti-inflamatorio y anti-artrítico de algunos extractos de plantas
DOI:
https://doi.org/10.3989/gya.125010Palabras clave:
Albahaca, Anti-inflamatorio, Anti-artrítico, Apio, Eucalipto, SalviaResumen
El objetivo de la presente investigación ha sido estudiar la actividad anti-inflamatoria de flores de albahaca dulces, hojas de eucalipto, hojas de apio y salvia. Se ha estudiado el efecto sobre la inflamación aguda de extractos metanólicos de apio, salvia y eucalipto y sobre la artritis inducida en ratas. Se han evaluado los efectos de los extractos metanólicos de apio, salvia y eucalipto sobre los parámetros bioquímicos determinados en la artritis inducida y su seguridad en las funciones del hígado y el riñón. Se han estudiado los ácidos grasos, hidrocarburos y fitoesteroles de todas las plantas objeto de la investigación. Los resultados han revelado una actividad antiinflamatoria de los extractos de las plantas estudiadas con diferentes grados entre un 47 y un 62%. Los extractos metanólico de apio, de salvia y de eucalipto han mostrado una mejoría de los niveles de malondialdehído en plasma, factor de necrosis tumoral α y de ácido úrico, también se muestra una mejoría significativa en el peso corporal y en la ingesta total de alimentos. Los extractos metanólicos de apio, de salvia y de eucalipto han mostrado una total seguridad sobre la función hepática y renal. Los ácidos α-linolénico y linoleico estaban presentes en todas las plantas estudiadas así como los esteroles Estigmasterol y β-sitosterol.
Descargas
Citas
Alleva LM, Cai C, Clark I.A. 2010. Using complementary and alternative medicines to target the host response during severe influenza. Evidence-Based Complementary and Alternative Medicine. 7, 501–510. http://dx.doi.org/10.1093/ecam/nep152 PMid:19779008 PMCid:2892358
A.O.A.C. 2000. Official Methods of Analysis (17th ed.). (Association of Official Agricultural Chemists, Gaithersburg, MD).
Belfield A, Goldberg DM. 1971. Revised assay for serum phenyl phosphate activity using 4-amino-antipyrine. Enzyme 12, 561-573. PMid:5169852
Bistrian BR, Blackburn GL. 1983. Assessment of proteincalorie malnutrition in the hospitalized patient. In: Nutritional Support of Medical Practice. (eds. H.A. Schneider, C.E. Anderson, D.B. Coursin) Harper and Row, New York, 128-153.
Bouic PJ. 2001. The role of phytosterols and phytosterolins in immune modulation: a review of the past 10 years. Curr. Opin. Clin. Nutr. Metab. Care. 4, 471-475. http://dx.doi.org/10.1097/00075197-200111000-00001 PMid:11706278
Bouic PJ, Lamprecht JH. 1999. Plant sterols and sterolins: a review of their immune-modulating properties. Altern. Med. Rev. 4, 170-7. PMid:10383481
Brennan FM, McInnes IB. 2008. Evidence that cytokines play a role in rheumatoid arthritis. Journal of Clinical Investigation 118, 3537–3545. http://dx.doi.org/10.1172/JCI36389 PMid:18982160 PMCid:2575731
Briggs GM, Williams MA. 1963. A new mineral mixture for experimental rat diets and evaluation of other mineral mixtures. Fed. Proc. 22, 261-266.
Calder PC. 1998. Dietary fatty acids and the immune system. Nutr. Rev. 56, S70-S83. http://dx.doi.org/10.1111/j.1753-4887.1998.tb01648.x
Calder PC, Yaqoob P. 2009. Understanding omega-3 polyunsaturated fatty acids. Postgrad Med. 121, 148-57. http://dx.doi.org/10.3810/pgm.2009.11.2083 PMid:19940425
Chang JM, Cheng CM, Hung LM, Chung YS, Wu RY. 2010. Potential use of plectranthus amboinicus in the treatment of rheumatoid arthritis. Evidence-Based Complementary and Alternative Medicine. 7, 115–120. http://dx.doi.org/10.1093/ecam/nem168 PMid:18955284 PMCid:2816374
Conseil de l’Europe: Pharmacopée Européenne. France: Maisonneuve S.A.: Sainte Ruffine 1996.
De Grauw JC, van de Lest CH, van Weeren PR. 2009. Inflammatory mediators and cartilage biomarkers in synovial fluid after a single inflammatory insult: a longitudinal experimental study. Arthritis Res. Ther. 11, R35. http://dx.doi.org/10.1186/ar2640
Dillard CJ, German JB. 2000. Phytochemicals and human health. J. Sci. Food Agric. 80, 1744-1756. http://dx.doi.org/10.1002/1097-0010(20000915)80:12<1744::AID-JSFA725>3.0.CO;2-W
DiRosa M, Willoughby DA. 1971. Screens for antiinflammatory drugs. J. Pharm. Pharmac. 23, 297-298. http://dx.doi.org/10.1111/j.2042-7158.1971.tb08661.x PMid:4102520
Fagan JM, Waxman L, Goldberg AL. 1987. Skeletal muscle and liver contain a soluble ATP + ubiqutin-dependent protedytic system. Biochem. J. 243, 335 – 340. PMid:2820375 PMCid:1147859
Fawcett JK, Scott JE. 1960. A rapid and precise method for the determination of urea. J. Clin. Pathol. 13, 156-159. http://dx.doi.org/10.1136/jcp.13.2.156 PMid:13821779 PMCid:480024
Glenn EM, Kooyers WM. 1966. Plasma inflammation units: An objective method for investigating effects of drugs on experimental inflammation. Life Sci. 5, 619-628. http://dx.doi.org/10.1016/0024-3205(66)90293-1
Gómez MA, Sáenz MT, García MD, Fernández MA. 1999. Study of the topical anti-inflammatory activity of Achillea ageratum on chronic and acute inflammation models. Z. Naturforsch [C]. 54, 937–941.
Gorman CL, Cope AP. 2008. Immune-mediated pathways in chronic inflammatory arthritis. Best Practice and Research: Clinical Rheumatology 22, 221–238. http://dx.doi.org/10.1016/j.berh.2008.01.003
Granado M, Priego T, Martín AI, Villanúa MA., López-Calderón A. 2005. Ghrelin receptor agonist GHRP-2 prevents arthritis-induced increase in E3 ubiquitinligating enzymes MuRF1 and MAFbx gene expression in skeletal muscle. American Journal of Physiology, Endocrinology and Metabolism 289, E1007–E1014. http://dx.doi.org/10.1152/ajpendo.00109.2005 PMid:16030067
Heffner JE, Repine JE. 1989. Pulmonary strategies of antioxidant defence. Am. Rev. Respir. Dis. 140, 531. http://dx.doi.org/10.1164/ajrccm/140.2.531
Holmdahl R, Lorentzen JC, Lu S, Olofsson P, Wester L, Holmberg J, Pettersson U. 2001. Arthritis induced in rats with nonimmunogenic adjuvants as models for rheumatoid arthritis. Immunol. Rev. 184, 184-202. http://dx.doi.org/10.1034/j.1600-065x.2001.1840117.x PMid:12086312
Houot O. 1985. Interpretation of clinical laboratory tests. Edit. Siest G, Henny J, Schiele F, Young D S. Biomedical publications.
Ibáñez de Cáceres I, Villanúa M, Soto L, Martín AI, López-Calderón A. 2000. Insulin like growth factor (IGF-I) and IGF-binding proteins in rats with adjuvantinduced arthritis given recombinant human growth hormone. J. Endocrinol 165, 537–544. http://dx.doi.org/10.1677/joe.0.1650537 PMid:10828837
Jayasinghe C, Gotoh N, Aoki T, Wada S. 2003. Phenolics composition and antioxidant activity of sweet basil (Ocimum basilicum L.). J. Agric. Food Chem. 51, 4442-9. http://dx.doi.org/10.1021/jf034269o PMid:12848523
Joffe I, Epstein S. 1991. Osteoporosis associated with rheumatoid arthritis: pathogenesis and management. Semin Arthritis Rheum. 20, 256-272. http://dx.doi.org/10.1016/0049-0172(91)90021-Q
Jung SM, Schumacher HR, Kim H, Kim M, Lee SH, Pessler F. 2007. Reduction of urate crystal-induced inflammation by root extracts from traditional oriental medicinal plants: elevation of prostaglandin D2 levels. Arthritis Research and Therapy. 9, R64. http://dx.doi.org/10.1186/ar2222
Kim SY, Park E, Park JA, Choi BS, Kim S, Jeong G, Kim CS, Kim do K, Kim SJ, Chun HS. 2010. The plant phenolic diterpene carnosol suppresses sodium nitroprusside-induced toxicity in c6 glial cells. J. Agric. Food Chem. 58, 1543-50. http://dx.doi.org/10.1021/jf903294x PMid:20073473
Kremers HM, Nicola P, Crowson CS, O’Fallon WM, Gabriel SE. 2004. Therapeutic strategies in rheumatoid arthritis over a 40-year period. Journal of Rheumatology 31, 2366–2373. PMid:15570636
Lanhers CM, Fleurentin J, Mortier F, Vinche A, Younos C. 1992. Anti-inflammatory and analgesic effects of aqueous extract of Harpagophtum procumbens. Planta Med. 58, 117. http://dx.doi.org/10.1055/s-2006-961411 PMid:1529021
Lecker SH, JagoeRT,Gilbert A,GomesM,BaracosV, Bailey J, Price SR, Mitch WE, Goldberg AL. 2004. Multiple types of skeletalmuscle atrophy involve a common program of changes in gene expression. FASEB Journal 18, 39–51. http://dx.doi.org/10.1096/fj.03-0610com PMid:14718385
Lipsky P. E. 2005. “Rheumatoid arthritis,” in Harrison’s Principles of Internal Medicine, D. Kasper, E. Braunwald, A. Fauci, S. Hauser, D. Longo, and J. Jameson, Eds., pp. 1968–1977, McGraw-Hill, New York, NY, USA, 16th edition.
Löliger J. 1991. The use of antioxidants in food. In Free radicals and food additives. Aruoma, OI.; Halliwell, B. Eds. Taylor and Francis, London, pp. 129- 138.
López-Lázaro M. 2009. Distribution and biological activities of the flavonoid luteolin. Mini Rev. Med. Chem. 9, 31-59. http://dx.doi.org/10.2174/138955709787001712 PMid:19149659
Lugasi A, Hòvári J, Sági KV, Birò L. 2003. The role of antioxidant phytonutrients in the prevention of diseases. Acta Biologica Szegediensis 47, 119-125.
Mahajan SG, Mali RG, Mehta AA. 2007. Protective Effect of Ethanolic Extract of Seeds of Moringa oleifera Lam. against Inflammation Associated with Development of Arthritis in Rats. J. Immunotoxicol 4, 39-47. http://dx.doi.org/10.1080/15476910601115184 PMid:18958711
Makino T, Ono T, Muso E, Yoshida H, Honda G, Sasayama S. 2000. Inhibitory effects of rosmarinic acid on the proliferation of cultured murine mesangial cells. Nephrol Dial Transplant 15, 1140-5. http://dx.doi.org/10.1093/ndt/15.8.1140
Martín de Santa Olalla L, Sánchez Muniz FJ, Vaquero MP. 2009. N-3 fatty acids in glucose metabolism and insulin sensitivity. Nutr. Hosp. 24, 113-27. PMid:19593479
Mohamed DA, Ismael AI, Ibrahim AR. 2005. Studying the anti-inflammatory and biochemical effects of wheat germ oil. Deutsche Lebensmittel-Rundschau 101, 66-72.
Mohamed DA, Al-Okbi SY. 2008. Evaluation of anti-gout activity of some plant foods. Pol. J. Food Nutr. Sci. 58, 389-395.
Morcos SR. 1967 The effect of protein value of the diet on the neurological manifestations produced in rats by b–immodipropionitrile. Br. J. Nutr. 21, 269-274. http://dx.doi.org/10.1079/BJN19670029
Nozaki K, Hikiami H, Goto H, Nakagawa T, Shibahara N, Shimada Y. 2006. Keishibukuryogan (Gui-Zhi-Fu-Ling-Wan), a Kampo formula, decreases disease activity and soluble vascular adhesion molecule-1 in patients with rheumatoid arthritis. Evidence-Based Complementary and Alternative Medicine 3, 359–364. http://dx.doi.org/10.1093/ecam/nel025 PMid:16951720 PMCid:1513153
Popovic M, Kaurinovic B, Trivic S, Mimica-Dukic N, Burac M. 2006. Effect of celery (Apium graveolens) extracts on some biochemical parameters of oxidative stress in mice treated with carbon tetrachloride. Phytother Res. 20, 531-7. http://dx.doi.org/10.1002/ptr.1871 PMid:16685681
Prior RL. 2003. Fruits and vegetables in the prevention of cellular oxidative damage. Am. J. Clin. Nutr. 78, 570S–578S. PMid:12936951
Qnais EY, Abu-Dieyeh M, Abdulla FA, Abdalla SS. 2010. The antinociceptive and anti-inflammatory effects of Salvia officinalis leaf aqueous and butanol extracts. Pharm. Biol. 48, 1149-56. http://dx.doi.org/10.3109/13880200903530763 PMid:20860437
Reitman S, Frankel S. 1957 Colorimetric methods for aspartate and alanine aminotransferase. Am. J. Clin. Path. 28, 55-60.
Saso L, Valentini G, Casini ML, Mattei E, Braghiroli L, Mazzanti G, Panzironi C, Grippa E, Silvestrini B. 1999. Inhibition of protein denatruation by fatty acids, bile salts and other natural substances: a new hypothesis for the mechanism of action of fish oil in rheumatic diseases. Jpn. J. Pharmacol.79, 89-99. http://dx.doi.org/10.1254/jjp.79.89
Satoh K. 1978. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clinica Chimica Acta. 20, 37-43.
Salvioli S, Sikora E, Cooper EL, Franceschi C. 2007. Curcumin in cell death processes: a challenge for CAM of age-related pathologies. Evidence-Based Complementary and Alternative Medicine 4, 181–190. http://dx.doi.org/10.1093/ecam/nem043 PMid:17549234 PMCid:1876609
Selibert K, Zhang Y, Leahy K, Hauser S, Masferrer J, Perkins W, Lee L, Isakson P. 1994. Pharmacological and biochemical demonstration of the role of cyclooxygenase 2 in inflammation and pain. Proc. Natl. Acad. Sci., USA. 91, 12013. http://dx.doi.org/10.1073/pnas.91.25.12013
Sevenian A, Davies KJA, Hochstein PA. 1985. Conservation of vitamin C by uric acid in blood. J. Free Rad. Biol. Med. 1, 117. http://dx.doi.org/10.1016/0748-5514(85)90015-7
Singh GB, Singh S, Bani S, Gupta BD, Banerje SK. 1992. Anti-inflammatory activity of oleanolic acid in rats and mice. J. Pharm. Pharmacol. 44, 456-8. http://dx.doi.org/10.1111/j.2042-7158.1992.tb03646.x PMid:1359067
Stepaniak JA, Gould KE, Sun D, Swanborg RH. 1995. A comparative study of experimental autoimmune encephalomyelitis in Lewis and DA rats. J. Immunol. 155, 2762-2769. PMid:7544385
Tastekin N, Aydogdu N, Dokmeci D, Usta U, Birtane M, Erbas H, Ture M. 2007. Protective effects of L-carnitine and alpha-lipoic acid in rats with adjuvant arthritis. Pharmacol Res. 56, 303-10. http://dx.doi.org/10.1016/j.phrs.2007.07.008 PMid:17826175
Van Ryn Y, Trummlitz G, Pairet M. 2000. COX-2 selectivity and inflammatory processes. Current Medicinal Chem. 7, 1145. PMid:11032964
Venkatraman J, Meksawan K. 2002. Effect of dietary omega-3 and omega-6 lipids and vitamin E on chemokine levels in autoimmune-prone MRL/MpJ-lpr/lpr mice. J. Nutr. Biochem. 13, 479. http://dx.doi.org/10.1016/S0955-2863(02)00201-2
Watts RE. 1974. Determination of uric acid in blood and urine. Ann. Clin. Biochem. 11, 103-111. PMid:4608659
Descargas
Publicado
Cómo citar
Número
Sección
Licencia
Derechos de autor 2011 Consejo Superior de Investigaciones Científicas (CSIC)

Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
© CSIC. Los originales publicados en las ediciones impresa y electrónica de esta Revista son propiedad del Consejo Superior de Investigaciones Científicas, siendo necesario citar la procedencia en cualquier reproducción parcial o total.
Salvo indicación contraria, todos los contenidos de la edición electrónica se distribuyen bajo una licencia de uso y distribución “Creative Commons Reconocimiento 4.0 Internacional ” (CC BY 4.0). Consulte la versión informativa y el texto legal de la licencia. Esta circunstancia ha de hacerse constar expresamente de esta forma cuando sea necesario.
No se autoriza el depósito en repositorios, páginas web personales o similares de cualquier otra versión distinta a la publicada por el editor.








