Enzyme-aided cold pressing of flaxseed (<i>Linum usitatissimum</i> L.): Enhancement in yield, quality and phenolics of the oil

F. Anwar; Z. Zreen; B. Sultana; A. Jamil

doi:10.3989/gya.132212

Autores/as

F. Anwar Department of Chemistry, University of Sargodha - Department of Chemistry and Biochemistry, University of Agriculture
Z. Zreen Department of Chemistry and Biochemistry, University of Agriculture
B. Sultana Department of Chemistry and Biochemistry, University of Agriculture
A. Jamil Department of Chemistry and Biochemistry, University of Agriculture

DOI:

https://doi.org/10.3989/gya.132212

Palabras clave:

Ácidos fenólicos, Carbohidrasas, HPLC, Linolénico, Parámetros físico-químicos, Prensado en frío de aceite, Propiedades antioxidante, Tocoferoles, TPC

Resumen

Se evalúa el efecto de diferentes preparaciones enzimáticas (Viscozyme L, Kemzyme y Feedzyme) sobre el rendimiento y propiedades fisicoquímicas y antioxidantes de aceites de lino prensados en frío. El rendimiento en aceite (35,2-38,0%) de las semillas de lino prensadas en frío (ETCPF), y tratadas con enzimas, aunque menor que el rendimiento mediante Soxhlet (SEO), fue considerablemente mayor en comparación con el control (32,5%), mientras que el contenido de proteína, fibra, y cenizas no se vieron afectados por el tratamiento enzimático. La mayoría de los parámetros físico-químicos tales como el índice de refracción, densidad, índice de yodo, el contenido de ácidos grasos libres, índice de saponificación, el color y el perfil de ácidos grasos no variaron significativamente entre el aceite ETCPF, SEO y el control. Curiosamente, el estado de oxidación en términos de peróxidos, p-anisidina, dienos y trienos conjugados, período de inducción (método Rancimat), así como puntuación sensorial del aceite ETCPF fueron superiores en comparación con el control. Una cantidad sensiblemente superior de tocoferoles (350-400 mg kg^–1) se determinó en el aceite ETCPF, en relación con el control (270 mg kg^–1), mostrando un aumento de 22,8 a 32,5% en la recuperación de los tocoferoles totales. Por otra parte, el aceite de ETCPF mostró mayor actividad antioxidante y fenoles totales y contenido de ácidos fenólicos individuales. Este estudio aboga por la extracción mediante presión en frío con enzima asistida como una alternativa viable al prensado en frío convencional para mejorar no sólo el rendimiento de extracción sino también la calidad de los componentes funcionales de alto valor como los de los aceites de linaza.

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Citas

Abdulkarim SM, Long K, Lai OM, Muhammad SKS, Ghazali HM. 2005. Some physico-chemical properties of Moringa oleifera seed oil extracted using solvent and aqueous enzymatic methods. Food Chem. 93, 253-263. http://dx.doi.org/10.1016/j.foodchem.2004.09.023

American Oil Chemists Society (AOCS). 1997. Official and Recommended Practices of the American Oil Chemists Society, 5th edn., AOCS Press, Champaign, Illinois, USA.

Association of Official Analytical Chemists (AOAC). 1990. Official Methods of Analysis of the Association of Official Analytical Chemists, 15th edition, AOAC Inc., Virginia.

Anwar F, Latif S, Przybylski R, Sultana B, Ashraf M. 2007. Chemical composition and antioxidant activity of seeds of different cultivars of mungbean. J. Food Sci. 72, S503-S510. http://dx.doi.org/10.1111/j.1750-3841.2007.00462.x PMid:17995664

Anwar F., Przybylski R. 2012. Effect of solvents extraction on total phenolics and antioxidant activity of extracts from flaxseed (Linum usitatissimum L.). Acta Sci. Pol. Technol. Aliment. 11, 293-301. PMid:22744950

Bylund A, Saarinen N, Zhang JX, Bergh A, Widmark A, Johansson A, Lundin E, Adlercreutz H, Hallmans G, Stattin P, Makela S. 2005. Anticancer effects of a plant lignan 7-hydroxymatairesinol on a prostate cancer model in vivo. Experimental Biol. Medicine. 230, 217-223.

Daun JK, Barthet VJ, Chornick TL, Dugiud S.2003. Structure, composition and variety development of flaxseed. Thompson I. U., Cannane, S.C. (Eds.), Flaxseed in Human Nutrition. AOCS Press. Champaign, USA, 1- 40.

Gao, M. and Liu, CZ. 2006. Dynamic microwave assisted extraction of flavonoids from Saussurea medusa maximum culture cells. J. Biochem. Eng. 32, 79-83. http://dx.doi.org/10.1016/j.bej.2006.09.004

Herchi W, Sawlha S, Arraez D, Boukhchina S, Carretero A, Kallel H, utierrezz AF. 2011. Determination of phenolic and other polar compounds in flax seed oil using liquid chromatography with time- of -flight mass spectrometry. Food Chem. 126, 332-338. http://dx.doi.org/10.1016/j.foodchem.2010.10.070

Hosseinian FS, Muir AD, Westcott ND, Krol ES. 2006. Antioxidant capacity of flaxseed lignans in two model systems. J. Am. Oil Chem. Soc. 83, 835-840. http://dx.doi.org/10.1007/s11746-006-5034-x

Hussain S, Anjum FM, Alamri MS. 2011. Fortification of pan bread with healthy flaxseed. Aus. J. Basic. App. Sci. 5, 978-983.

International Standards Organization (ISO). Animal Feeding Stuffs -Determination of Nitrogen and Calculation of Crude Protein Content; ISO: Geneva, Switzerlanld, 1981; Standard 5983.

International Standards Organization (ISO). Oilseed Residues- Determination of Total Ash; ISO: Geneva, Switzerland, 1977; Standard 749.

IUPAC. Standard Methods for the Analysis of Oils, Fats and Derivatives, 7th revised Edn. International Union of Pure and Applied Chemistry, Blackwell Scientific, London 1987.

Kaufmann B, Christen P. 2002. Recent extraction techniques for natural products: microwave-assisted extraction and pressurised solvent extraction. Phytochem. Anal. 13, 105-113. http://dx.doi.org/10.1002/pca.631 PMid:12018022

Kouba M. 2006. Effect of dietary omega-3 fatty acids on meat quality of pigs and poultry. In: MCT Eagle (ed). Omega 3 Fatty Acid Research. Nova Publishers. New York, USA, pp. 225-239

Latif S, Anwar F. 2011. Aqueous enzymatic sesame oil and protein extraction. Food Chem. 125, 679-684. http://dx.doi.org/10.1016/j.foodchem.2010.09.064

Latif S, Anwar F, Ashraf M. 2007. Characterization of enzyme-assisted cold pressed cotton seed oil. J. Food Lipids. 14, 424-436. http://dx.doi.org/10.1111/j.1745-4522.2007.00097.x

Latif S, Anwar F, Hussain AI, Shahid M. 2011. Aqueous enzymatic process for oil and protein extraction from Moringa oleifera seed, Eur. J. Lipid Sci. Technol. 11, 1012-1018 http://dx.doi.org/10.1002/ejlt.201000525

Latif S, Anwar F. (2009). Physico-chemical studies of hemp (Cannabis sativa) seed oil using enzymeassisted cold pressing. Eur. J. Lipid Sci. Technol. 10, 1042-1048. http://dx.doi.org/10.1002/ejlt.200900008

Maillard V, Bougnoux P, Ferrari P, Jourdan ML, Pinault M, Lavillonniere F, Body G, Le Floch O, Chajes V. 2002. n-3 and n-6 fatty acids in breast adipose tissue and relative risk of breast cancer in a case-control study in Tours France. Int. J. Cancer. 98:78-83 http://dx.doi.org/10.1002/ijc.10130 PMid:11857389

Matthews KR, Homer DB, Thies F, Calder PC.2000. Effect of whole linseed (Linum usitatissimum L) in the diet of finishing pigs on growth performance and on the quality and fatty acid composition of various tissues. British J. Nutrition. 83, 637-643. http://dx.doi.org/10.1017/S0007114500000817 PMid:10911772

Mc Cann SE, Kulkarni S, Trevisan M, Vito D, Nie J, Edge SB, Muti P, Freundenheim JL. 2006. Dietary lignan intakes and risk of breast cancer by tumor estrogen receptor status. Breast Cancer Res. Treatment. 99, 309-311. http://dx.doi.org/10.1007/s10549-006-9196-x PMid:16541305

Min DB. 1983. Analyses of flavor qualities of vegetable oils by gas chromatography. J. Am. Oil Chem. Soc. 60, 544-545. http://dx.doi.org/10.1007/BF02679783

Moure A, Dominguez H, Zuniga ME, Carmen S, Chamy R. 2002. Characterization of protein concentrates from pressed cakes of Guevina avellana (Chilean hazelnut). Food Chem. 78, 179-186. http://dx.doi.org/10.1016/S0308-8146(01)00397-1

Oomah B D. 2001. Flaxseed as a functional food source. J. Agric. Food Chem. 81, 889-894. http://dx.doi.org/10.1002/jsfa.898

Parry J, SU L, Luther M, Zhou K, Yurawecz MP, Whittaker P, 2005. Fatty acid composition and antioxidant properties of cold-pressed marionberry, boysenberry, red raspberry, and blueberry seed oils. J. Agric. Food Chem. 53, 566-573. http://dx.doi.org/10.1021/jf048615t PMid:15686403

Ranalli A, De Mattia G. 1997. Characterization of olive oil produced with a new enzyme processing aid. J. Am Oil Chem. Soc., 74, 1105-1113. http://dx.doi.org/10.1007/s11746-997-0032-1

Ranalli A, Malfatti A, Lucera L, Contento S, Sotiriou E. 2005. Effects of processing techniques on the natural colorings and the other functional constituents in virgin olive oil. Food Res. Int. 38, 873-878. http://dx.doi.org/10.1016/j.foodres.2005.02.011

Riley PA, Enser M, Nute GR, Wood JD. 2000. Effects of dietary linseed on nutritional value and other quality aspects of pig muscle and adipose tissue. Animal Sci. 71, 483-500.

Schaefer EJ. 2002. Lipoproteins, nutrition, and heart disease. Am. J Clin. Nutr. 75, 191-212 PMid:11815309

Siger A, Malgorzata NK, Eleonora LS. 2008. The content and antioxidant activity of phenolic compounds in cold-pressed plant oils. J. Food Lipids 15, 137-149. http://dx.doi.org/10.1111/j.1745-4522.2007.00107.x

Singh RK, Sarker BC, Kumbhar BK. 1999. Response surface analysis of enzyme assisted oil extraction factors for sesame, groundnut and sunflower seeds. J. Food Sci. Technol. 36, 511-514.

Soto CG, Chamy R, Zuniga M. 2004. Effect of enzymatic application on Borage (Borago officinalis) oil extraction by cold pressing. J. Chem. Eng. Japan. 37, 326-331. http://dx.doi.org/10.1252/jcej.37.326

Steel, F, Torrie GJH and Dickey DA. 1997. Principals and procedures of biometrical approach 3rd edition. W. C. McGraw Hill, New York

Tarpila A, Wennberg T, Tarpila S, 2005. Flaxseed as a functional food. Curr. Top. Nutrac. Res. 3, 167-188.

Tzen JTC, Huang AHC. 1992. Surface structure and properties of plant seed oil bodies. J. of biological chem. 117, 327-335.

Westcott ND, Muir AD. 2003. Flaxseed lignan in disease prevention and health promotion. Phytochem. Rev. 2, 401-417. http://dx.doi.org/10.1023/B:PHYT.0000046174.97809.b6

Wrolstad RE. 2003 in: Wrolstad, R. E. (Ed.), Current Protocols in Food Analytical Chemistry (CPFA), John Wiley & Sons, UK, pp. 6902-6904.

Yen GC, Duh PD, Chuang DY. 2000. Antioxidant activity of anthraquinones and anthrone. Food Chem. 70, 307-315. http://dx.doi.org/10.1016/S0308-8146(00)00108-4

Zuniga ME, Chamy R, Lema JM. 2001. Canola and Chilean hazelnut products obtained by enzymeassisted cold-pressed oil extraction. In Proceedings of the world conference and exhibition on oilseed processing and utilization (R.F. Wilson, ed.). AOCS Press, Champaign, pp. 210-213.