Extracellular laccase production and phenolic degradation by an olive mill wastewater isolate

R. Kumar; Y. Raizner; L. I. Kruh; O. Menashe; H. Azaizeh; S. Kapur; E. Kurzbaum

doi:10.3989/gya.0776171

Autores/as

R. Kumar Shamir Research Institute, University of Haifa - Department of Biological Science, Birla Institute of Technology and Science https://orcid.org/0000-0001-6929-9176
Y. Raizner Shamir Research Institute, University of Haifa https://orcid.org/0000-0001-7710-5845
L. I. Kruh Department of Biotechnology Engineering, ORT Braude College https://orcid.org/0000-0002-8342-7653
O. Menashe Water Industry Engineering Department, Achi Racov Engineering School, Kinneret College on the Sea of Galilee https://orcid.org/0000-0001-7368-0754
H. Azaizeh Institute of Applied Research, University of Haifa - Tel-Hai College, Department of Environmental Science https://orcid.org/0000-0002-0311-1158
S. Kapur Department of Biological Science, Birla Institute of Technology and Science https://orcid.org/0000-0002-1941-9288
E. Kurzbaum Shamir Research Institute, University of Haifa https://orcid.org/0000-0002-8258-9407

DOI:

https://doi.org/10.3989/gya.0776171

Palabras clave:

Acinetobacter REY, Aguas residuales de almazara, Biodegradación, Compuestos fenólicos, Lacasa de células extracelulares

Resumen

Las aguas residuales de almazara (OMWW) presentan un desafío a los efluentes debido a la presencia de una carga orgánica alta, agentes antimicrobianos (fenoles monoméricos y poliméricos, ácidos volátiles, polialcoholes y taninos), salinidad y acidez. En este estudio, se investigó la producción de lacasa extracelular a partir de un aislado de OMWW basado en su capacidad para biodegradar fenol y ácido gálico como modelo de compuestos fenólicos en OMWW. El análisis filogenético de las secuencias del gen de ARN 16S identifico el aislado bacteriano (Acinetobacter REY) como el más cercano a Acinetobacter pittii. Este aislado exhibió producción constitutiva de lacasa extracelular con una actividad de 1.5 y 1.3 U mL/L cuando se suplemento con los inductores CuSO₄ y CuSO₄ + fenol, respectivamente. Se realizaron experimentos en lotes que contenían medios mínimos suplementados con fenol o acido gálico como la única fuente de carbono y energía con el fin de caracterizar su biodegradabilidad fenólica. Acinetobacter REY fue capaz de biodegradar hasta 200 mg/L de fenol y acido gálico después de 10 y 72 h, respectivamente.

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Citas

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