Investigation on chemical composition, antioxidant activity and SARS-CoV-2 nucleocapsid protein of endemic Ferula longipedunculata Peşmen

A.  Göçeri; İ.  Demirtaş; M.H.  Alma; Ş.  Adem; Z.A.  Kasra; F.  Gül; A.  Uzun

doi:10.3989/gya.0107211

Authors

A. Göçeri Department of Bioengineering and Science, Scientific Research Center, Erbil Polytechnic University https://orcid.org/0000-0002-1218-6696
İ. Demirtaş Department of Biochemistry, Faculty of Science and Arts, Igdir University https://orcid.org/0000-0001-8946-647X
M.H. Alma Igdir University https://orcid.org/0000-0001-6323-7230
Ş. Adem Department of Chemistry, Faculty of Sciences, Çankırı Karatekin University https://orcid.org/0000-0003-2146-5870
Z.A. Kasra Department of Bioengineering and Science, Salahaddin University-Erbil https://orcid.org/0000-0002-9762-8093
F. Gül Department of Property, Protection and Security, Igdir Vocational School, Igdir University https://orcid.org/0000-0002-4297-786X
A. Uzun Faculty of Forestry, Department of Forest Botany, Kahramanmaraş Sutcu İmam University https://orcid.org/0000-0002-2577-7460

DOI:

https://doi.org/10.3989/gya.0107211

Keywords:

Antioxidants, Chemical composition, Ferula longipedunculata Peşmen, COVID 19, SARS-CoV-2

Abstract

The essential and fatty oils were investigated and a quantitative analysis of the root, green and stem parts of F. Longipedunculata was performed by GC-MS and HPLC-TOF/MS and their antioxidant (DPPH method) activities and potential binding of phytochemicals against SARS-CoV-2 nucleocapsid were determined using Molegro Virtual Docker software. In the root part of the plant, the prominent components of oil were β-phellandrene (53.46%), ocimene (6.79%), 4-terpineol (5.94%) and santalol (5.03%). According to the quantitative results, vanillic acid (141.35 mg/kg), ferulic acid (126.19 mg/kg) and 4-hydroxybenzoic acid (119.92 mg/kg) were found in the roots; quercetin-3-β-O-glycoside (1737.70 mg/kg), quercetin (531.35 mg/kg) and ferulic acid (246.22 mg/kg) were found in the in the green part; and fumaric acid (2100.21 mg/kg), quercetin-3-β-O-glycoside (163.24 mg/kg), vanillic acid (57.59 mg/kg) were detected in the stem part. The antioxidant activity of all parts of the plant was higher than the control with BHT. Silibinin, rutin, and neohesperidin exhibited a stronger affinity than nucleotides. In the silico analysis, many of the phytochemicals were attached with strong hydrogen-bonds and electrostatic effects to the amino acids to which nucleotides are bound. The results indicated that the plant showed antioxidant effects and can be effective against SARS-CoV-2 thanks to the different phytochemical compounds it contains.

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