Biodiesel production from animal grease wastes by enzymatic catalysis

Authors

  • Ivanna Rivera Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ)
  • Gerardo Villanueva Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ)
  • Georgina Sandoval Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ)

DOI:

https://doi.org/10.3989/gya.021409

Keywords:

Biodiesel, Ethyl esters, Grease waste, Lipase, Solvent free system, Transesterification

Abstract


Animal grease wastes were successfully used in the transesterification reaction as an alternative raw material for biodiesel production. Transesterification reactions were performed using an enzymatic catalysis with two commercial lipases N435 and RM IM. N435 was the most efficient in a solvent free system as well as in the system with terbuthanol. In both systems the excess of ethanol turned out to be beneficial for the reaction obtaining conversions of 80% in 48 hours and 65% in 24 hours.

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References

Al-Zuhair S. 2005. Production of biodiesel by lipasecatalyzed transesterification of vegetable oils: A kinetics study. Biotechnol Progress 21, 1442-1448. doi:10.1021/bp050195k PMid:16209548

AOAC International. 1984. Official methods of analysis. Peroxide value: 28.022 – 28.023.

AOAC International. 1989. Official methods of analysis. Free fatty acid: Ca 5a-40.

Badui Dergal S. 1993. Química de los alimentos. Tercera edición. Pearson Educación. 213-232. Naucalpan Edo. México. México.

Balat M, Balat H. 2008. A critical review of bio-diesel as a vehicular fuel Energy Convers Manage 49, 2727- 2741. doi:10.1016/j.enconman.2008.03.016

Canakci M. 2005. The potential of restaurant waste lipids as biodiesel feedstocks. Bioresour Technol. 11, 1-8.

Canakci M, Sanli H. 2008. Biodiesel production from various feedstocks and their effects on the fuel properties. J. Ind. Microbiol & Biotechnol 35, 431-441. doi:10.1007/s10295-008-0337-6

Chulalaksananukul W, Condoret JS, Delorme P, Willemot RM. 1990. kinetic-study of esterification by immobilized lipase in n-hexane. Febs. Letters 276, 181-184. doi:10.1016/0014-5793(90)80537-S PMid:2265698

Dizge N, Keskinler B. 2008. Enzymatic production of biodiesel from canola oil using immobilized lipase. Biomass Bioenergy 32, 1274-1278. doi:10.1016/j.biombioe.2008.03.005

Du W. 2005. Study on acyl migration in immobilized lipozyme TL- catalized transesterificación of soybean oil for biodiesel production. J. Mol. Catal. B. Enzymatic 37, 68-71. doi:10.1016/j.molcatb.2005.09.008

Guncheva MH, Zhiryakova D. 2008. High-yield synthesis of wax esters catalysed by modified Candida rugosa lipase. Biotechnol Lett. 30, 509-512. doi:10.1007/s10529-007-9554-8 PMid:17957342

Haas MJ, McAloon AJ, Yee WC, Foglia TA. 2006. A process model to estimate biodiesel production cost. Bioresour Technol. 97,671-678. doi:10.1016/j.biortech.2005.03.039 PMid:15935657

Hsu AF, Jones KC, Foglia TA, Marmer WN. 2003. Optimization of alkyl ester production from grease using a phyllosilicate sol-gel immobilized lipase. Biotechnol Lett. 25, 1713-1716. doi:10.1023/A:1026094318940 PMid:14626413

Knothe G, Dunn RO, O. BM. 1996. Technical aspects of biodiesel standards. Inform. 7, 827-829

Lee T, Foglia T, Chang KS. 2002. Production of alkyl ester as biodiesel from fractionated lard and restaurant grease. JAOCS 79, 191-195. doi:10.1007/s11746-002-0457-y

Meher LC, Sagar DV, Naik SN. 2006. Technical aspects of biodiesel production by transesterification – a review. Renew Sustain Energy Rev. 10, 248-268. doi:10.1016/j.rser.2004.09.002

Royon D, Daz M, Ellenrieder G, Locatelli S. 2007. Enzymatic production of biodiesel from cotton seed oil using t-butanol as a solvent. Bioresour Technol. 98, 648-653. doi:10.1016/j.biortech.2006.02.021 PMid:16630719

Sandoval G, Condoret JS, Marty A. 2001. Thermodynamic Activity Based Enzyme Kinetics: an efficient tool for nonaqueous enzymology. AIChE J. 47, 718-726. doi:10.1002/aic.690470318

Sandoval G, Monsan P, Marty A. 2002. Esterification by immobilized lipases: kinetic and thermodynamic arguments. Biotechnol Bioeng. 78, 313-320. doi:10.1002/bit.10224 PMid:11920447

Schmid RD, Verger R. 1998. Lipases: Interfacial Enzymes with Attractive Applications. Angrew. Chem. Int. ed. 37, 1608-1633

Yagiz F, Kazan D, Akin AN. 2007. Biodiesel production from waste oils by using lipase immobilized on hydrotalcite and zeolites. J. Chem. Eng. 134, 262-267 doi:10.1016/j.cej.2007.03.041

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Published

2009-12-30

How to Cite

1.
Rivera I, Villanueva G, Sandoval G. Biodiesel production from animal grease wastes by enzymatic catalysis. Grasas aceites [Internet]. 2009Dec.30 [cited 2024Apr.16];60(5):470-6. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/600

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