Grasas y Aceites, Vol 59, No 2 (2008)

Conjugated linoleic acid (CLA). Cis 9, trans 11 and trans 10, cis 12 isomer detection in crude and refined corn oils by capillary GC


https://doi.org/10.3989/gya.2008.v59.i2.503

Özlem Tokuşoğlu
Celal Bayar University, Akhisar M.Y.O., Turkey

Abstract


Conjugated linoleic acids (CLAs) exhibit protective effects against various types of cancer and heart diseases. With the newly developed capillary gas chromatographic method (GC), cis9, trans11 and trans10, cis12 octadecadienoic acid isomers of CLA (C18:2) were determined in crude and refined corn oils as qualitative and quantitative measurements. Cis 9, trans11 C18:2 (c9, t11 CLA) was the major CLA isomer in both oils. It was found that c9, t11 CLA was 0.62% of the total lipid in crude oil and 1.24% of the total lipid in refined oil. Using the refining process, the total CLA was 1.38% whereas that of crude corn oil was 0.62%. An approximate 2.2 fold increase in the total CLA was found in refined oil (n = 9) (p < 0.01). The alteration in CLA could be used as a quality indicator for the refining process due to the contrubution of CLA to polyunsaturated fatty acids. Analytical method validation, calibration (R2 = 0.9999) and recovery data [y = 2.782x + 0.046 (R2 = 0.9999)] were performed (p < 0.01).The proponed chromatographic procedure could be used for vegetable oil quality control

Keywords


Conjugated linoleic acid; Corn oil; GC; Isomer; Refining

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References


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