The antisense expression of AhPEPC1 increases seed oil production in peanuts (Arachis hypogaea L.)

Authors

  • L. Pan State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomic Sciences, Shandong Agricultural University - Shandong Peanut Research Institute
  • J. Zhang State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomic Sciences, Shandong Agricultural University - Shandong Peanut Research Institute
  • X. Chi Shandong Peanut Research Institute - Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences,
  • N. Chen Shandong Peanut Research Institute
  • M. Chen Shandong Peanut Research Institute
  • M. Wang Shandong Peanut Research Institute
  • T. Wang Shandong Peanut Research Institute
  • Z. Yang Shandong Peanut Research Institute
  • Z. Zhang Shandong Peanut Research Institute
  • Y. Wan State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomic Sciences, Shandong Agricultural University
  • S. Yu Shandong Peanut Research Institute
  • F. Liu State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomic Sciences, Shandong Agricultural University

DOI:

https://doi.org/10.3989/gya.0322161

Keywords:

Antisense expression, Lipid content, Peanut, Phosphoenolpyruvate carboxylase, Salt tolerance

Abstract


Although phosphoenolpyruvate carboxylases (PEPCs) are reported to be involved in fatty acid accumulation, nitrogen assimilation, and salt and drought stresses, knowledge regarding PEPC gene functions is still limited, particularly in peanuts (Arachis hypogaea L.). In this study, the antisense expression of the peanut PEPC isoform 1 (AhPEPC1) gene increased the lipid content by 5.7%–10.3%. This indicated that AhPEPC1 might be related to plant lipid accumulation. The transgenic plants underwent more root elongation than the wild-type under salinity stress. Additionally, the specific down regulation of the AhPEPC1 gene improved the salt tolerance in peanuts. This is the first report on the role of PEPC in lipid accumulation and salt tolerance in peanuts.

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References

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Published

2016-12-30

How to Cite

1.
Pan L, Zhang J, Chi X, Chen N, Chen M, Wang M, Wang T, Yang Z, Zhang Z, Wan Y, Yu S, Liu F. The antisense expression of AhPEPC1 increases seed oil production in peanuts (Arachis hypogaea L.). grasasaceites [Internet]. 2016Dec.30 [cited 2023Feb.5];67(4):e164. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1630

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Research

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