The antisense expression of <em>AhPEPC1</em> increases seed oil production in peanuts (<em>Arachis hypogaea</em> L.)

L. Pan; J. Zhang; X. Chi; N. Chen; M. Chen; M. Wang; T. Wang; Z. Yang; Z. Zhang; Y. Wan; S. Yu; F. Liu

doi:10.3989/gya.0322161

Autores/as

L. Pan State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomic Sciences, Shandong Agricultural University - Shandong Peanut Research Institute
J. Zhang State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomic Sciences, Shandong Agricultural University - Shandong Peanut Research Institute
X. Chi Shandong Peanut Research Institute - Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences,
N. Chen Shandong Peanut Research Institute
M. Chen Shandong Peanut Research Institute
M. Wang Shandong Peanut Research Institute
T. Wang Shandong Peanut Research Institute
Z. Yang Shandong Peanut Research Institute
Z. Zhang Shandong Peanut Research Institute
Y. Wan State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomic Sciences, Shandong Agricultural University
S. Yu Shandong Peanut Research Institute
F. Liu State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomic Sciences, Shandong Agricultural University

DOI:

https://doi.org/10.3989/gya.0322161

Palabras clave:

Cacahuete, Contenido de lípidos, Expresión antisentido, Fosfoenolpiruvato carboxilasa, Tolerancia a la sal

Resumen

Aunque se ha demostrado que las carboxilasas fosfoenolpiruvato (PEPCs) están implicadas en la acumulación de ácidos grasos, en la asimilación de nitrógeno, y en el estrés salino e hídrico, el conocimiento respecto a las funciones del gen PEPC es todavía limitado, particularmente en cacahuete (Arachis hypogaea L.). En este estudio, la expresión antisentido de la isoforma (AhPEPC1) del gen PEPC 1 de cacahuete aumentó el contenido de lípidos en un 5,7%–10,3%. Esto indica que AhPEPC1 podría estar relacionado con la acumulación de lípidos de plantas. Las plantas transgénicas experimentaron una mayor elongación de las raíces que la de tipo silvestre bajo estrés por salinidad. Además, la específica baja regulación del gen AhPEPC1 mejoró la tolerancia a la sal en cacahuete. Este es el primer informe sobre el papel del gen PEPC en la acumulación de lípidos y la tolerancia a la sal en cacahuete.

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