Soybean phospholipase D activity determination. A comparison of two methods

E. Tosi; G. Ballerini; E. Ré

doi:10.3989/gya.2007.v58.i3.182

Soybean phospholipase D activity determination. A comparison of two methods

Authors

E. Tosi Centro de Investigación y Desarrollo en Tecnología de Alimentos (CIDTA), Facultad Regional Rosario - Universidad Tecnológica Nacional
G. Ballerini Centro de Investigación y Desarrollo en Tecnología de Alimentos (CIDTA), Facultad Regional Rosario - Universidad Tecnológica Nacional
E. Ré Centro de Investigación y Desarrollo en Tecnología de Alimentos (CIDTA), Facultad Regional Rosario - Universidad Tecnológica Nacional

DOI:

https://doi.org/10.3989/gya.2007.v58.i3.182

Keywords:

Hydratable phosphatides, Non hydratable phosphatides, Phospholipase D, Phospholipase D activity, Soybean

Abstract

Due to a discrepancy between previously published results, two methods to determine the soybean phospholipase D activity were evaluated. One method is based on the extraction of the enzyme from whole soybean flour, quantifying the enzyme activity on the extract. The other method quantifies the enzymatic activity on whole soybean flour without enzyme extraction. In the extraction-based-method, both the extraction time and the number of extractions were optimized. The highest phospholipase D activity values were obtained from the method without enzyme extraction. This method is less complex, requires less running-time and the conditions of the medium in which phospholipase D acts resemble the conditions found in the oil industry

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References

Bergmeyer HU, Gavehn K. 1974. Methods of enzymatic analysis. 2nd Ed., Academic Press., New York, pp. 415-418.

List G, Mounts T, Lanser C, Holloway A. 1990. Effects of moisture, microwave heating and live steam treatment on phospholipase D activity in soybeans and soy flakes. JAOCS 67, 867-871. doi:10.1007/BF02540508

List G, Mounts T, Lanser C. 1992. Factors promoting the formation of nonhydratable soybean phosphatides. JAOCS 69, 443-446. doi:10.1007/BF02540945

Nakayama K, Saio K, Kito M. 1981. Decomposition of phospholipids in soybean during storage. Cereal Chem. 58, 260-264.

Stuckey J, Dixon J. 1999. Crystal structure of a phospholipase D family member. Nat. Struct. Mol. Biol. 8 (3), 278-284.

Tosi E, Cazzoli A, Ré E, Tapiz L. 1999. Soyben Drying in fluidized bed. Effect on the hydratable and nonhydratable phophatides. Concentration in crude and degummed crude oil. JAOCS 78 (10), 1467-1470

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Published

2007-09-30

How to Cite

Tosi E, Ballerini G, Ré E. Soybean phospholipase D activity determination. A comparison of two methods. Grasas aceites [Internet]. 2007Sep.30 [cited 2025Feb.22];58(3):270-4. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/182

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Issue

Vol. 58 No. 3 (2007)

Section

Research

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