Analyses and evaluation of the main chemical components in different tobacco (Nicotiana tabacum L.) genotypes




Fatty acid, Nicotine, Sugar, Tobacco


The nicotine, reducing sugar and ion contents from the threshing of tobacco can re-used from the industry. The crude oil and fatty oil compositions of tobacco seeds can be considered as an alternative source of raw material for biodiesel. In this study, the nicotine, reducing sugar content, crude oil, fatty acid composition and ion content were determined in 29 genotypes and 1 cultivar of tobacco. The genetic diversity was determined among the tobacco cultivar and genotypes base on examined properties. The nicotine content varied between 0.10-0.87%, reducing sugar ranged from 9.70-21.30%, crude oil varied between 24.33-47.00% and fatty acid compositions was found in the range of 77.94-100%. Linoleic (13.92-75.04%) and butyric (0.33-64.98%) acids were the major components. Overall, the BSR-5 (52.56 mg/g) and ESR-5 (44.58 mg/g) genotypes exhibited the highest potassium contents and ESR-7 (6.54 mg/g) and ESR-8 (1.28 mg/g) genotypes had the lowest chlorine contents. As a result of this study, the highest nicotine content, reducing sugar and crude oil of tobacco were found in ESR-4, ESR-11 and BSR-5 genotypes, respectively. The dendrogram analysis divided the tobacco into two main groups and most of the same origin genotypes fell into the same group. The results indicated that the different tobacco leaves and seeds can be evaluated as an alternative source in the industry as cigarettes, biodiesel and different industrial applications such as cosmetic, oil paints and varnishes based on their chemical properties.


Download data is not yet available.


Abbas Ali M, Abu Sayeed M, Roy RK. 2008. Comparative study of on characteristics of Seeds oils and Nutritional composition of seeds from different varieties of tobacco cultivated in Bangladesh. Asian J. Biochem. 3 (4), 203-212.

Abdallah F. 1986. Is Tobacco Quality Measurable? Trans. K. Ketenci. Monopoly Institute Istanbul.

ARGEFAR. 2016. Center for Drug Research & Development and Pharmaceutical Applications, Ege University., 21.08.2018.

Awola VG, Ogunniyi DS, Odetoye TE. 2010. Refining, modification and characterization of tobacco seed oil for improved potentials for industrial use. Nigerian J. Pure Appl. Sci. 2168-2174.

Bastida G, Beltran B. 2011. Ulcerative colitis in smokers, non-smokers and ex- smokers. World J. Gastroenterol. 17, 2740-2747. PMid:21734782 PMCid:PMC3122262

Bilgin AE, Müftüoğlu Y, Ustralı A. 1993. Calibration of Commercial Tobacco Requirements and Phosphorus-Potash Analysis Methods of Oriental Tobacco Under Field Conditions in Aegean Region. T. C. Ministry of Agriculture and Rural Affairs General Directorate of Rural Services Menemen Research Institute Publications. Publication No: 1995, İzmir. 1993.

Bucciarelli S, Civitella D, Fecondo G, Ghiann G, Sorrentino C, Del Piano L, Raimo F, Stanisci V. 2013. Influence of plant density and leaf priming on seed and seed oil yield tobacco. Proc. CORESTA Meeting, Agronomy/Phytopathology, Brufa di Torgiano (PG), Italy.

Cao Y, Li HQ, Zhang J. 2011. Homogeneous synthesis and characterization of cellulose acetate butyrate (CAB) in 1-Allyl-3- methylimidazolium chloride (AmimCl) ionic liquid. Indust. Engineer. Chem. Res. 50 (13), 7808-7814.

Camas N, Karaali H, Ozcan H. 2007. Effects of different fertilizer doses on yield, quality and technological properties of pressed tobacco genotype in Erbaa-Taşova Conditions. 2007 TTL Foreign Trade. Research Report.

Chiririwa H, Hapanyengwi A, Muzenda E. 2014. Tobacco seed oil as an economically viable alternative for the tobacco ındustry. International Conference on Chemical Engineering and Advanced Computational Technologies, November, 24-25, 36-39.

Delibacak S, Ongun AR, Ekren S. 2014. Influence of soil properties on yield and quality of tobacco plant in Akhisar region of Turkey. Eurasian J. Soil Sci. 3, 286-292.

Dwidar M, Park JY, Mitchell RJ, Sang B. 2012. The future of butyric acid in ındustry. Scient. World J. 1-9. PMid:22593687 PMCid:PMC3349206

Ekren S, Sekin S. 2008. Investigation of chemical and expertise characteristics of Akhisar region tobaccos and relations with the leaf yield. Ege J. Agric. Res. 45 (3), 165-173.

Er C, Yıldız M. 2014. Pleasure plant. Ankara University Publications, No: 419.

Fornasier F, Gomez JFC, de Carvalho FS, Schneider RdCdS, Costa ABd, Moraes JAR, Bravo dCAG. 2018. Biodiesel production from energy tobacco. Orbital: Electronic J. Chem. 10 (2), 123-132.

Giannelos PN, Zannikos F, Stourna S, Lois E, Anastopoulos G. 2002. Tobacco seed oil as an alternative diesel fuel: physical and chemical properties. Ind. Crops Prod. 16, 1-9.

Griesser M, Weingart G, Schoedl-Hummel K, Neumann N, Becker M, Varmuza K, Liebner F, Schuhmacher R, Forneck A. 2015. Severe drought stress is affecting selected primary metabolites, polyphenols, and volatile metabolites in grapevine leaves (Vitis vinifera cv. Pinot noir). Plant. Physiol. Biochem. 88, 17-26. PMid:25602440

Irget ME, Oktay M, Hakerlerler H, Atil H, Cakici H. 1999. A research on the nutrition and soilplant relationships of Virginia (flue-cured) tobacco grown in Düzce. J. AARI 9 (2), 125-142.

Kirkova S, Srbinoska M, Ivanova S, Georgieva A. 2016. Determination of fatty acid composition of seed of oriental tobacco. Tobacco 66 (1-6), 53-58.

Krishnamurthy V, Ramakrishnayya BV. 1993. Yield and quality of fcv tobacco as affected by potassium nutrition. pp. 78-102. In: Plant nutrition effects on production and quality of tobacco. Potash and Phosphate Inst. of Canada. India Programme Sector-19, Haryana.

Kurt D, Ayan AK. 2014. Effect of the different organic fertilizer sources and doses on yield in organic tobacco (Nicotiana tabacum L.) production. J. Agric. Faculty Gaziosmanpasa Univer. 31 (2), 7-14.

Mohammad MT, Tahir NA. 2014. Evaluation of chemical compositions of tobacco (Nicotiana tabacum L) genotypes. Seeds Annual Res. Review Biol. 4 (9), 1480-1489.

Otan H, Apti R. 1989. Tobacco. T.C. T.O.K.İ.B. Ege Agricultural Research Institute Publications, No: 83. Menemen-İzmir.

Palomer X, Pizarro-Delgado J, Barroso E, Vázquez-Carrera M. 2018. Palmitic and oleic acid: The yin and yang of fatty acids in type 2 diabetes mellitus. Trends Endocrinol. Metabol. 29 (3), 178-190. PMid:29290500

Piano L del, Abet M, Raimo F, Modestia F, Sicignano M, Enotrio T. 2014b. Caraterizzazione morfologica e qualitativa di accessioni di Nicotiana tabacum L. per la produzione di olio da seme. Proc. X National Congress on Biodiversity, Rome, Italy.

Poltronieri P. 2016. Tobacco seed oil for biofuels. Pp. 161-185. In: Poltronieri P and D'Urso O.F. Biotransformation of Agricultural Waste and By-Products. The Food, Feed, Fibre, Fuel

(4F) Economy. Elsevier, Oxford, UK. 2016. ISBN: 9780128036228.

Regassa R, Chandravanshi BS. 2016. Levels of heavy metals in the raw and processed Ethiopian tobacco leaves. Springer Plus 5, 232 PMid:27026926 PMCid:PMC4771704

Stanisavljević IT, Veličković DT, Todorović ZB, Lazić ML, Veljković VB. 2009. Comparison of techniques for extraction of tobacco seed oil. Europ. J. Lipid Sci. Technol. 111 (5), 513-518.

Talaqani TE, Shafik J, Mustafa FK. 1986. Fatty acid composition of the seed oil of certain tobacco varieties cultivated in Northern Iraq. Indian J. Agric. Chem. 19, 147-154.

TEA. 2018. Tobacco Experts Association. Chemical structure of tobacco leaf in terms of blending., 21.08.2018.

Wang M, Fu Y, Liu H. 2016. Nutritional quality and ions uptake to PTNDS in soybeans. Food Chem. 192, 750-759. PMid:26304407

Yagac C. 2015. Determination of yield and quality characters of tobacco cultivars of Aegean Region in Denizli conditions. MsC Namık Kemal University Graduate School of Natural and Applied Sciences Department of Field Crops.

Yaldiz G, Çamlica M. 2019. Variation in the fruit phytochemical and mineral composition, and phenolic content and antioxidant activity of the fruit extracts of different fennel (Foeniculum vulgare L.) genotypes. Ind. Crops Prod. 142, 111852.

Yaldiz G, Camlica M, Ozen F. 2019. Biological value and chemical components of essential oils of sweet basil (Ocimum basilicum L.) grown with organic fertilization sources. J. Sci. Food Agric. 99, 2005-2013. PMid:30393851

Zalatanov M, Menkov N. 2000. Phospholipid and fatty acid composition of tobacco seeds. Rostlinna Vyroba 46, 439-441.

Zandalinas SI, Mittler R, Balfagón D, Arbona V, Gómez-Cadenas A. 2018. Plant adaptations to the combination of drought and high temperatures. Physiol. Plant. 162, 2-12. PMid:28042678



How to Cite

Camlica M, Yaldiz G. Analyses and evaluation of the main chemical components in different tobacco (Nicotiana tabacum L.) genotypes. grasasaceites [Internet]. 2021Feb.24 [cited 2022Nov.28];72(1):e389. Available from:




Funding data

Abant Izzet Baysal Üniversitesi
Grant numbers BAP-2016.10.07.1090