The biodegradation of Olive Oil Mill Wastewaters by Sawdust and by a Phanerochaetae chrysosporium


  • M. Mebirouk Ecole Mohammadia d’Ingénieurs (E.M.I), Département Génie Minéral, Laboratoire de Biorémédiation
  • L. Sbai Ecole Mohammadia d’Ingénieurs (E.M.I), Département Génie Minéral, Laboratoire de Biorémédiation
  • M. Lopez Universidad de Granada, Facultad de Farmacia, Departemento de Nutricion y Bromatologia
  • J. Gonzalez Universidad de Granada, Instituto de Agua



Biodegradation, Decolorization, Olive mill wastewaters, Polyphenols, Sawdust


This paper discusses decolorization and chemical oxygen demand (COD) abatement in olive mill wastewaters (OMW) by Phanerochaetae chrysosporium grown in static, stirred and immobilized cultures. When P. Chrysosporium is used in cultures, no decolorization of crude OMW is observed. Decolorization occurs only after the removal of polyphenols by adsorption in sawdust, which allows a 39% polyphenol removal. The use of a High lignin peroxides (Lip) producing medium, yields the highest OMW decolorization and COD removal efficiencies. The use of P. Chrysosporium immobilized on polyurethane foam leads to significant abatements of OMW polluting characteristics. And COD abatement reached 70%. The reduction of polyphenols reached its highest level at 62%. A significant effluent decolorization is apparent.


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APHA, Standard Methods for Water and Wastewater Examination, 17th ed. 1989 Amer.Public Health Assoc., Washington, D.C

Dutta S, Basu JK, Ghar RN. 2001. Studies on adsorption of p-nitrophenol on charred Saw-dust. Separation and Purification Technology. 21, 227-235 . doi:10.1016/S1383-5866(00)00205-7

Espensen JH. 1981. Chemical Kinetics and reaction mechanism. McGraw-Hill: New York.

Hernandez MT, Ramos CA, Martinez J. 1990. Bacteria degrading phenolic acids isolated on a polymeric pigment. J. Appl. Bacteriol. 69, 38-42.

Kirk TK, Farrell RL. 1987. Enzymatic ‘‘combustion’’: the microbial degradation of lignin. Annu. Rev. Microbiol. 41, 465–505. doi:10.1146/annurev.mi.41.100187.002341

Maestro R, Borja R, Martin A, Fiestas J, Alba J. 1991. Biodegradacion de los compuestos phenolicos presentes en el alpechin. Grasas y Aceites. 42, 271-276.

Mebirouk M, Sbai ML, Skalli A. 2007. Phenols Biodegradation in olive mill waste-waters factorial experiments analysis. Statist. Probab. Letters (in press).

Sáinz JC, Gómez AG, de Leeuw JW. 1986. Chemical properties of the polymer isolated in fresh vegetation water and sludge evaporation ponds. In: International Symposium on Olive By Products Valorization., Madrid, Spain. Food and Agriculture Organization (ed.), Publication Division of the United Nations, pp. 41-60.

Sayadi S, Ellouz R. 1992. Decolourization of olive mill wastewaters by the white rot fungus Phanerochaetae chrysosporium: involvement of the lignin-degrading system. Appl. Microbiol. Biotechnol. 37, 813-817. doi:10.1007/BF00174851

Sayadi S, Ellouz R. 1993. Screening of white rot fungi for the treatment of olive mill wastewaters. J. Chem.Tech. Biotechnol. 57, 141-147.

Sayadi S, Zorgani F, Ellouz R. 1996. Decolourization of olive mill wastewaters by free and immobilized Phanerochaetae chrysosporium cultures: effect of the high molecular weight polyphenols. Appl. Bioch. Biotechnol. 56, 265-276.




How to Cite

Mebirouk M, Sbai L, Lopez M, Gonzalez J. The biodegradation of Olive Oil Mill Wastewaters by Sawdust and by a Phanerochaetae chrysosporium. grasasaceites [Internet]. 2007Dec.30 [cited 2022Dec.6];58(4):366-71. Available from: