Antioxidant activity, polyphenolic composition and in vitro antibacterial and antifungal activities of tea seed oil

H.K.  Ruto; J.O.  Yugi; S.O.  Ochanda; C.  Bii

doi:10.3989/gya.1229202

Authors

H.K. Ruto University of Kabianga, Biological sciences - Kenya Agricultural and Livestock Research organization, Tea Research Institute https://orcid.org/0000-0002-7067-9018
J.O. Yugi University of Kabianga - Biological sciences https://orcid.org/0000-0002-1937-6644
S.O. Ochanda Kenya Agricultural and Livestock Research organization - Tea Research Institute https://orcid.org/0000-0001-5035-6724
C. Bii Kenya Medical Research Institute - Centre for Respiratory Diseases Research https://orcid.org/0000-0002-5635-3871

DOI:

https://doi.org/10.3989/gya.1229202

Keywords:

Anti-microbial activity, Antioxidant activity, Catechins, Polyphenol, Tea seed oil

Abstract

The polyphenolic composition and antioxidant activity of tea seed oil from C. sinensis TRFK 301/5 (green colored) and TRFK 306 (purple colored) and C. oleifera were evaluated. The total polyphenolic content, total catechins and catechin fractions were significantly different in the oils. C. oleifera contained significantly (p ≤ 0.05) higher amounts of catechins and polyphenols than C. sinensis. C. oleifera also exhibited a higher DPPH radical scavenging activity (18.81 ± 0.46%) compared to C. sinensis (TRFK 306; 15.98 ± 0.13 and TRFK 301/5; 14.73 ± 0.47%). The antimicrobial activities of tea seed oil and two selected oils (olive and eucalyptus oil), were also evaluated against Escherichia coli, Staphylococcus aureus, Candinda albicans, Cryptococcus neoformans and Trichophyton mentagrophytes. S. aureus was significantly inhibited by the oils compared to E. coli. The oils inhibited the growth of T. mentagrophytes and C. albicans, although they had no effect on C. neoformans. Tea seed oil is a potential source of beneficial phytochemicals and potent antimicrobial agents.

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