Grasas y Aceites, Vol 71, No 1 (2020)

Hepatoprotective effect of the unsaponifiable matter from olive, linseed and sesame oils against carbon tetrachloride-induced liver injury in rats


https://doi.org/10.3989/gya.1175182

S. M. Galal
Department of Food Science, Faculty of Agriculture, Cairo University, Egypt
orcid https://orcid.org/0000-0002-1859-1641

M. K.S. Morsi
Department of Food Science, Faculty of Agriculture, Cairo University, Egypt
orcid https://orcid.org/0000-0003-2061-6075

M. K. Abd El-Rahman
National Nutrition Institute, Ministry of Health and Population, Egypt
orcid https://orcid.org/0000-0003-0474-3023

S. K. Darwish
National Organization for Drug Control and Research, Ministry of Health and Population, Egypt
orcid https://orcid.org/0000-0001-7480-160X

M. A. Katry
National Nutrition Institute, Ministry of Health and Population, Egypt
orcid https://orcid.org/0000-0002-2857-671X

Abstract


In the present study, the hepatoprotective activity of the unsaponifiable matter (UNSAP) of olive oil, linseed, and sesame oils against CCl4-induced liver toxicity in rats was investigated. In a preliminary antioxidant study, UNSAP showed pronounced DPPH radical scavenging activity (IC50 6.2-10.8 mg/mL). The constituents of UNSAP were determined by GC-MS. The subcutaneous administration of CCl4, caused liver injury. The hepatoprotective effect of UNSAP was comparable to that of α-tocopherol, a standard antioxidant agent. The co-administration of the investigated UNSAP normalized the activities of serum marker enzymes, alanine aminotransferase (ALT), and aspartate aminotransferase (AST). Furthermore, the serum alkaline phosphatase (ALP) activity and hepatic malondialdehyde (MDA) level were found to be alleviated by pre-treatment with the UNSAP. A histopathological evaluation showed marked improvement in the liver of UNSAP- and α-Tocopherol-treated animals. The hepatoprotective effect could be attributed to the antioxidant characteristics of UNSAP.

Keywords


CCl4; Hepatotoxicity; Histopathology; Rat; Unsaponifiable matter

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