Grasas y Aceites, Vol 71, No 1 (2020)

Hepatoprotective effect of the unsaponifiable matter from olive, linseed and sesame oils against carbon tetrachloride-induced liver injury in rats

S. M. Galal
Department of Food Science, Faculty of Agriculture, Cairo University, Egypt

M. K.S. Morsi
Department of Food Science, Faculty of Agriculture, Cairo University, Egypt

M. K. Abd El-Rahman
National Nutrition Institute, Ministry of Health and Population, Egypt

S. K. Darwish
National Organization for Drug Control and Research, Ministry of Health and Population, Egypt

M. A. Katry
National Nutrition Institute, Ministry of Health and Population, Egypt


In the present study, the hepatoprotective activity of the unsaponifiable matter (UNSAP) of olive oil, linseed, and sesame oils against CCl4-induced liver toxicity in rats was investigated. In a preliminary antioxidant study, UNSAP showed pronounced DPPH radical scavenging activity (IC50 6.2-10.8 mg/mL). The constituents of UNSAP were determined by GC-MS. The subcutaneous administration of CCl4, caused liver injury. The hepatoprotective effect of UNSAP was comparable to that of α-tocopherol, a standard antioxidant agent. The co-administration of the investigated UNSAP normalized the activities of serum marker enzymes, alanine aminotransferase (ALT), and aspartate aminotransferase (AST). Furthermore, the serum alkaline phosphatase (ALP) activity and hepatic malondialdehyde (MDA) level were found to be alleviated by pre-treatment with the UNSAP. A histopathological evaluation showed marked improvement in the liver of UNSAP- and α-Tocopherol-treated animals. The hepatoprotective effect could be attributed to the antioxidant characteristics of UNSAP.


CCl4; Hepatotoxicity; Histopathology; Rat; Unsaponifiable matter

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AOAC 2005. Official Methods of Analysis of AOAC international, 18th ed. AOAC international, Gaithersburg, USA.

AOCS 2009. Official Methods and Recommended Practices of the American Oil Chemists' Society, AOCS Press, Champaign, Illinois, USA.

Al-Seeni MN, El Rabey HA, Zamzami MA, Alnefayee AM. 2016. The hepatoprotective activity of olive oil and Nigella sativa oil against CCl4 induced hepatotoxicity in male rats. BMC Complement. Altern. Med. 16, 438-451. PMid:27814700 PMCid:PMC5097446

Bancroft JD, Steven A, Turner DR. 1996. Theory and Practice of Histological Techniques. Churchill Livingstone, New York, London, San Francisco, Tokyo.

Beji RS, Mansour RB, Rebey IB, Wannes WA, Jameleddine S, Hammami M, Megdiche W, Ksouri R. 2018. Does Curcuma longa root powder have an effect against CCl4- induced hepatotoxicity in rats: a protective and curative approach? Food Sci. Biotechnol. 27, 1-9.

Codex Alimentarius Commission 2015. Codex Standard for Named Standard for Edible Fats and Oils. Codex Stan 210-1999.

Echeverría F, Ortiz M, Valenzuela R, Videla LA. 2017. Hydroxytyrosol and cytoprotection: A projection for clinical interventions. Int. J. Mol. Sci. 18, 930. PMid:28452954 PMCid:PMC5454843

European Food Safety Authority. 2015. Scientific opinion on the re-evaluation of tocopherol-rich extract (E 306), ?-tocopherol (E 307), ?-tocopherol (E 308) and ?-tocopherol (E 309) as food additives. E. F. S. A. 13, 4247.

Farhoosh R, Tavassoli-Kafrani MH, Sharif A. 2013. Assaying antioxidant characteristics of sesame seed, rice bran, and bene hull oils and their unsaponifiable matters by using DPPH radical-scavenging model system. J. Agric. Sci. Tech. 15, 241-251.

Fontanel D. 2013. Unsaponifiable Matter in Plant Seed Oils. Springer-Verlag, Berlin Heidelberg. PMid:24451416

Fukuda Y, Nagata M, Osawa T, Namiki M. 1986. Chemical aspects of the antioxidative activity of roasted sesame seed oil, and the effect of using the oil for frying. Agric. Biol. Chem. 50, 857-862.

Gliszczynska-Swiglo A, Sikorska E, Khmelinskii I, Sikorski M. 2007. Tocopherol content in edible plant oils. Pol. J. Food Nutr. Sci. 57, 157-161.

Ham H, Lee YY, Park JY, Lee C, Kwak J, Kim IH, Lee J. 2016. Protective mechanisms of Unsaponifiable matter from rice bran against tert-butyl hydroperoxide-induced oxidative damage in HepG2 cells. J. Food Biochem. 40, 526-534.

Hendawi MY, Alam RT, Abdellatief SA. 2016. Ameliorative effect of flaxseed oil against thiacloprid-induced toxicity in rats: hematological, biochemical, and histopathological study. Environ. Sci. Pollut. Res. 23, 11855-11863. PMid:26957428

Illesca P, Valenzuela R, Espinosa A, Echeverría F, Soto-Alarcon S, Ortiz M, Videla LA. 2019. Hydroxytyrosol supplementation ameliorates the metabolic disturbances in white adipose tissue from mice fed a high-fat diet through recovery of transcription factors Nrf2, SREBP-1c, PPAR-? and NF-?B. Biomed. Pharmacother. 109, 2472-2481. PMid:30551508

Jain A, Soni M, Deb L, Jain A, Rout S, Gupta V, Krishna K. 2008. Antioxidant and hepatoprotective activity of ethanolic and aqueous extracts of Momordica dioica Roxb. leaves. J. Ethnopharmacol. 115, 61-66. PMid:17983713

Johnston DE, Kroening C. 1998. Mechanism of early carbon tetrachloride toxicity in cultured rat hepatocytes. Pharmacol. Toxicol. 83, 231-239. PMid:9868740

Krauß S, Vetter W. 2018. Phytol and phytyl fatty acid esters: occurrence, concentrations, and relevance. Eur. J. Lipid Sci. Technol. 120, 1-13.

Lai CY, Cheng SB, Lee TY, Liu HT, Huang SC, Huang YC. 2018. Possible synergistic effects of glutathione and C - reactive protein in the progression of liver cirrhosis. Nutrients 10, 678. PMid:29861471 PMCid:PMC6024608

Liu Q, Kong B, Li G, Liu N, Xia X. 2011. Hepatoprotective and antioxidant effects of porcine plasma protein hydrolysates on carbon tetrachloride-induced liver damage in rats. Food Chem. Toxicol. 49, 1316-1321. PMid:21414373

Moazzami AA, Haese SL, Kamal-Eldin A. 2007. Lignan contents in sesame seeds and products. Eur. J. Lipid Sci. Technol. 109, 1022-1027.

Narasinga Rao BS. 2001. Nonglyceride components of edible oils and fats. 2. Nutritional and health significance. Food Nutr. Bull. 22, 87-93.

Nicco C, Batteux F. 2018. ROS Modulator molecules with therapeutic potential in cancers treatments. Molecules 23, 1-16. PMid:29301225 PMCid:PMC6016996

Niranjan TG, Kumar MV, Lokesh BR, Krishnakantha TP. 1999. Influence of unsaponifiable matter from ghee on lymphocyte proliferation and erythrocyte fragility in rats. Nutr. Res. 19, 1671-1682.

Periasamy S, Liu CT, Chien SP, Chen YC, Liu MY. 2016. Daily sesame oil supplementation mitigates ketoconazole-induced oxidative stress-mediated apoptosis and hepatic injury. J. Nutr. Biochem. 37, 67-75. PMid:27619544

Ramadan MF, Showky HE, Sulieman AE. 2008. Comparison between the effect of ?-irradiation and roasting on the profile and antioxidant activity of wheat germ lipids. Grasas Aceites 59, 166-173.

Reddy LH, Couvreur P. 2009. Squalene: A natural triterpene for use in disease management and therapy. Adv. Drug Deliv. Rev. 61, 1412-1426. PMid:19804806

Wang L, Tu Z, Wang H, Wang S, Wang X, Zhu H, Hu C, Liu Y. 2018. Flaxseed oil improves liver injury and inhibits necroptotic and inflammatory signaling pathways following lipopolysaccharide challenge in a piglet model. J. Funct. Foods. 46, 482-489.

Yildiztekin F, Nadeem S, Erol E, Yildiztekin M, Tuna AL, Ozturk M. 2016. Antioxidant, anticholinesterase and tyrosinase inhibition activities, and fatty acids of Crocus mathewii - A forgotten endemic angiosperm of Turkey. Pharm. Biol. 54, 1557-1563. PMid:26810584

Zhou D, Ruan J, Cai Y, Xiong Z, Fu W, Wei A. 2010. Antioxidant and hepatoprotective activity of ethanol extract of Arachniodes exilis (Hance) Ching. J. Ethnopharmacol. 129, 232-237. PMid:20347029

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