Grasas y Aceites, Vol 55, No 4 (2004)

Utilisation of rapeseed protein isolates for production of peptides with angiotensin I-converting enzyme (ACE)-inhibitory activity


https://doi.org/10.3989/gya.2004.v55.i4.200

Justo Pedroche
Departamento de Fisiología y Tecnología de Productos Vegetales. Instituto de la Grasa (CSIC)., Spain

María M. Yust
Departamento de Fisiología y Tecnología de Productos Vegetales. Instituto de la Grasa (CSIC)., Spain

Cristina Megías
Departamento de Fisiología y Tecnología de Productos Vegetales. Instituto de la Grasa (CSIC)., Spain

Hassane Lqari
Departamento de Fisiología y Tecnología de Productos Vegetales. Instituto de la Grasa (CSIC)., Spain

Manuel Alaiz
Departamento de Fisiología y Tecnología de Productos Vegetales. Instituto de la Grasa (CSIC)., Spain

Julio Girón-Calle
Departamento de Fisiología y Tecnología de Productos Vegetales. Instituto de la Grasa (CSIC)., Spain

Francisco Millán
Departamento de Fisiología y Tecnología de Productos Vegetales. Instituto de la Grasa (CSIC)., Spain

Javier Vioque
Departamento de Fisiología y Tecnología de Productos Vegetales. Instituto de la Grasa (CSIC)., Spain

Abstract


ACE activity is related to increased arterial pressure and coronary diseases. A rapeseed protein isolate was hydrolyzed with the protease Alcalase in order to investigate the possible presence of ACE inhibitory peptides in the resulting hydrolysates. Hydrolysis for 30 min yielded a hydrolysate with the highest ACE inhibitory activity. Two fractions of this hydrolysate obtained by Biogel P2 gel filtration chromatography were used for further purification of ACE inhibitory peptides. Three fractions with ACE inhibitory activity were purified by reverse-phase HPLC of Biogel P2 f ractions. This demonstrates that rapeseed protein hydrolysates represent a good source of ACE inhibitory peptides .

Keywords


Angiotensin converting enzyme; Peptide inhibitors; Protein hydrolysate; Protein isolate; Rapeseed

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