A simple method for purification of deodorizer distillate from Indian rice (Oryza Sativa) bran oil and preparation of phytosterols
Keywords:Brown color and bad odor fraction (BCBOF), Compounds responsible for brown color of RBO DOD, High purity phytosterols, Phytosterol concentrate fraction (PCF)
Samples of rice bran oil deodorizer distillates (RBO DOD-1 and RBO DOD-2) were studied for their physicochemical characteristics. The samples were semisolid and had a dark color. The free fatty acid values were 59.2% and 86.0%, the unsaponifiable matter was 18.7% and 7.75% and the phytosterol contents were 8.71% and 4.22%, respectively for the deodorizer distillates studied. A simple method of silica gel percolation was developed to purify DOD to obtain phytosterol concentrate fractions (PCF) and a brown color and bad odor fraction (BCBOF). The color values were reduced by 72.8% and 73.0% of lovibond units in the PCF for DOD-1 and DOD-2 respectively, had no bad odor and were increased in the phytosterol concentration to 12.4% and 5.9%. The PCF was further processed to prepare high purity phytosterols. An HPLC analysis of the phytosterol mixture showed it to be formed by β-sitosterol (38.2%), stigmasterol (34.9%), campesterol (9.5%) and other sterols (17.4%).
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