Physicochemical characteristics and oxidative stability of Egyptian corn germ oil produced by aqueous enzymatic extraction
Keywords:Antioxidant and oxidative properties, Bacterial protease, Bovine protease, Corn germ oil, Fungal cellulase, Physicochemical properties
The physicochemical properties and the oxidative stability of Egyptian corn germ oil produced by aqueous enzymatic extraction using fungal cellulase, bacterial and bovine proteases, either individually or in combination were compared to that extracted by hexane. The optimized conditions of the new bovine protease for maximum oil yield were: 0.1% enzyme, pH 7.5 and 1h incubation time versus 0.6%, 0.63% enzyme, pH 4, 8.2 and 4, 2h incubation for fungal cellulase and bacterial protease, respectively. The higher oil yield was obtained by combining cellulase with bacterial protease (62.38%) or with bovine protease (51.94%) relative to hexane extraction (100%). The refractive index, iodine, saponification and peroxide values, DPPH scavenging activity, as well as fatty acids composition of enzymes and solvent-extracted oils were comparable. The acid value and color index of the enzyme-extracted oils were better than that produced by hexane, indicating that enzymes could be applied in the production of eco-friendly corn oil.
Anjum F, Anwar F, Jamil A, Iqbal M. 2006. Microwave roasting effects on the physico-chemical composition and oxidative stability of sunflower seed oil. J. Am. Oil Chem. Soc. 83, 777–784. https://doi.org/10.1007/s11746-006-5014-1
Anwar F, Ashraf M, Bhanger MI. 2005. Interprovenance variation in the composition of Moringa oleifera oilseeds from Pakistan. J. Am. Oil Chem. Soc. 82, 45–51. https://doi.org/10.1007/s11746-005-1041-1
AOAC, 2005. Official Methods of Analysis of the Association of Official Analytical Chemists. 18th Ed., Gaithersburg, USA.
AOCS, 2005 in: Firestone D. (Edn.), Official Methods and Recommended Practices of the American Oil Chemists' Society, AOCS Press, Champaign, IL. Methods Cd 1–25, Cd 3a-63, Cd 3–25 and Cd 8–53.
Balvardi M, Rezaei K, Mendiola JA, Ibá-ez E. 2015. Optimization of the aqueous enzymatic extraction of oil from Iranian wild almond. J. Am. Oil Chem. Soc. 92, 985– 992. https://doi.org/10.1007/s11746-015-2671-y
Barminas JT, James MK, Abubakar UM. 1999. Chemical composition of seeds and oil of Xylopia Aethiopica grown in Nigeria. Plant Food Hum. Nutr. 53, 193–198. https://doi.org/10.1023/A:1008028523118 PMid:10517278
Bender DA. 2009. A dictionary of food and nutrition. 3 rd edition, Oxford university press.
Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181, 1199–1200. https://doi.org/10.1038/1811199a0
Bocevska M, Karlovi? D, Turkulov J, Pericin D. 1993. Quality of corn germ oil obtained by aqueous enzymatic extraction. J. Am. Oil Chem. Soc. 70, 1273–1277. https://doi.org/10.1007/BF02564241
Brehm BJ, Lattin BL, Summer SS, Boback JA, Gilchrist GM, Jandacek RJ, D'alessio DA. 2009. One-year comparison of a high–monounsaturated fat diet with a high-carbohydrate diet in type 2 diabetes. Diabetes Care 32, 215–220. https://doi.org/10.2337/dc08-0687 PMid:18957534 PMCid:PMC2628682
Burlakova EB, Alesenko AV, Molochkina EM, Palmina NP, Khrapova NG. 1975. Bioantioxidants in radiation damage and malignant growth. Moscow: Nauka (in Russian).
Calder PC. 2013. Omega-3 polyunsaturated fatty acids and inflammatory processes: nutrition or pharmacology?. Br. J. Clin. Pharmacol. 75, 645–662. https://doi.org/10.1111/j.1365-2125.2012.04374.x PMid:22765297 PMCid:PMC3575932
Calder PC. 2015. Marine omega-3 fatty acids and inflammatory processes: effects, mechanisms and clinical relevance. Biochim. Biophys. Acta 1851, 469–484. https://doi.org/10.1016/j.bbalip.2014.08.010 PMid:25149823
Choe E, Min DB. 2006. Mechanisms and factors for edible oil oxidation. Comprehensive reviews in food science and food safety 5, 169–186. https://doi.org/10.1111/j.1541-4337.2006.00009.x
Finucane OM, Lyons CL, Murphy AM, Reynolds CM, Klinger R, Healy NP, Cooke AA, Coll RC, McAllan L, Nilaweera KN, O'Reilly ME. 2015. Monounsaturated fatty acid– enriched high-fat diets impede adipose NLRP3 inflammasome–mediated IL-1? secretion and insulin resistance despite obesity. Diabetes 64, 2116–2128. https://doi.org/10.2337/db14-1098
Formo MW, Jungermann E, Norris FA, Sonntag NOV. 1979. Baily's Oil and Fat Products. 4th edn. by D.Swern, Interscience Publishers, New York, USA. PMid:90581
Huang AH. 1996. Oleosins and oil bodies in seeds and other organs. Plant Physiology 110, 1055–1061. https://doi.org/10.1104/pp.110.4.1055 PMid:8934621 PMCid:PMC160879
Johnston DB, McAloon AJ, Moreau RA, Hicks KB, Singh V. 2005. Composition and economic comparison of germ fractions from modified corn processing technologies. J. Am. Oil Chem. Soc. 82, 603–608. https://doi.org/10.1007/s11746-005-1116-z
Kowalski B, Ratusz K, Kowalska D, Bekas W. 2004. Determination of the oxidative stability of vegetable oils by differential scanning calorimetry and Rancimat measurements. Eur. J. Lipid Sci. Technol. 106, 165–169. https://doi.org/10.1002/ejlt.200300915
Lamas DL, Crapiste GH, Constenla DT. 2014. Changes in quality and composition of sunflower oil during enzymatic degumming process. LWT - Food Sci. Technol. 58, 71–76.
Latif S, Anwar F. 2009. Effect of aqueous enzymatic processes on sunflower oil quality. J. Am. Oil Chem. Soc. 86, 393– 400. https://doi.org/10.1007/s11746-009-1357-8
Latif S, Anwar F. 2011. Aqueous enzymatic sesame oil and protein extraction. Food chem. 125, 679–684. https://doi.org/10.1016/j.foodchem.2010.09.064
Mehanni AES, El-Reffaei WHM, Melo A, Casal S, Ferreira IM. 2017. Enzymatic Extraction of Oil from Balanites Aegyptiaca (Desert Date) Kernel and Comparison with Solvent Extracted Oil. J. Food Biochem. 41.
Mojtaba A, Fardin K. 2013. Optimization of enzymatic extraction of oil from Pistacia Khinjuk seeds by using central composite design. Food Sci. Technol.º 1, 37–43.
Moral PS, Méndez MVR. 2006. Production of pomace olive oil. Grasas Aceites 57, 47–55.
Moreau RA, Dickey LC, Johnston DB, Hicks KB. 2009. A process for the aqueous enzymatic extraction of corn oil from dry milled corn germ and enzymatic wet milled corn germ (E-Germ). J. Am. Oil Chem. Soc. 86, 469–474. https://doi.org/10.1007/s11746-009-1363-x
Moreau RA, Johnston DB, Dickey LC, Parris N, Hicks KB. 2007. Aqueous enzymatic oil extraction: a ''green'' bioprocess to obtain oil from corn germ and other oil-rich plant materials, in: Eggleston G, Vercellotti JR (eds). ºThe industrial application of enzymes on carbohydrate based materials. American Oil Chemists' Society Press, Champaign, 101–120.
Moreau RA, Johnston DB, Powell MJ, Hicks KB. 2004. A comparison of commercial enzymes for the aqueous enzymatic extraction of corn oil from corn germ. J. Am. Oil Chem. Soc. 81, 1071–1075. https://doi.org/10.1007/s11746-004-1023-3
Pearson D. 1976. Chemical Analysis of Foods. 7th Edn., Church Hill Livingstone, London, UK, 72–73,138–143, 488–496.
Pons WA, Kuck JC, Frampton VL. 1960. Color index for cottonseed oils. J. Am. Oil Chem. Soc. 37, 671–673. https://doi.org/10.1007/BF02632095
Rao MB, Tanksale AM, Ghatge MS, Deshpande VV. 1998. Molecular and biotechnological aspects of microbial proteases. Microbiol. Mol. Biol. Rev. 62, 597–635. PMid:9729602 PMCid:PMC98927
Shende D, Sidhu EK. 2014. Methods Used for Extraction of Maize (Zea Mays L.) Germ Oil-A Review. Ind. J. Sci. Res. and Tech. 2, 48–54.
Tirzitis G, Bartosz G. 2010. Determination of antiradical and antioxidant activity: basic principles and new insights. Acta Biochimica Polonica 57, 139–142. PMid:20454707
Turan S, Topcu A, Karabulut I, Vural H, Hayaloglu AA. 2007. Fatty acid, triacylglycerol, phytosterol, and tocopherol variations in kernel oil of Malatya apricots from Turkey. J. Agric. Food Chem. 55, 10787–10794. https://doi.org/10.1021/jf071801p PMid:18038980
Wirasnita R, Hadibarata T, Novelina YM, Yusoff ARM, Yusop Z. 2013. A modified methylation method to determine fatty acid content by gas chromatography. Bull. Korean Chem. Soc. 34, 3239–3242. https://doi.org/10.5012/bkcs.2013.34.11.3239
Xie M, Dunford NT, Goad C. 2011. Enzymatic extraction of wheat germ oil. J. Am. Oil Chem. Soc. 88, 2015–2021. https://doi.org/10.1007/s11746-011-1861-5
Yu L, Haley S, Perret J, Harris M, Wilson J, Qian M. 2002. Free radical scavenging properties of wheat extracts. J. Agric. Food Chem. 50, 1619–1624. https://doi.org/10.1021/jf010964p PMid:11879046
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