Antioxidant activity of extracts from Sclerocarya birrea kernel oil cake


  • Abdalbasit A. Mariod Food Sci & Technology Department, College of Agricultural Studies, Sudan University of Science & Technology
  • Bertrand Matthäus Institute for Lipid Research, Federal Research Center for Nutrition and Food
  • K. Eichner Institute for Food Chemistry, Westfälische-Wilhelms-Universität
  • Ismail H. Hussein National Oilseed Processing Research Institute (NOPRI), University of Gezira



Antioxidant activity, ß-carotene-linoleic acid assay, Kernel oil meal, Methanolic extracts, Phenolic compounds, Sclerocarya birrea


The antioxidant activity of methanolic extracts from Sclerocarya birrea kernel oil meal, extracted using two different methods was evaluated. The extraction was carried out using magnetic stirring of the material in methanol/water (80:20 v/v) overnight followed by two ultra-sonic treatments for 45 min. (Overnight extract, ONEXT) and three ultra-sonic treatments for 45 min. only (Ultra-sonic extract, USEXT), respectively. Three fractions were obtained from each extract and the contents of total phenolic compounds were determined in each fraction according to the Folin-Ciocalteau method as 34.6, 54.8, and 58.6 mg/g of dry product in ONEXT and 29.6, 84.8, 143.9 mg/g in USEXT, respectively. The antioxidant activity of the extracts was evaluated according to the β-carotene-linoleic acid assay, where the extracts and their fractions showed significant effect (p<0.05). The antioxidative properties of the extracts obtained from the two extraction methods described were similar. The AAC (antioxidant activity coefficient) of these extracts and their fractions increased with an increasing concentration of the extract. The effect of ONEXT and USEXT at the 0.2 and 0.8 % levels on the oxidative stability of sunflower oil at 70°C was tested in the dark and compared with the commonly used synthetic antioxidant BHA. The oil peroxide values (PVs) were significantly (p<0.05) lower with the addition of extract in comparison to a control. In comparison to BHA (0.02%) the increase of PVs after the addition of ONEXT (0.2% and 0.8%) and USEXT (0.8%), respectively, was reduced. The oxidation of sunflower oil, treated with 0.2%, 0.5%, and 1.0% of ONEXT and USEXT, respectively, was tested using the Rancimat test at 120 °C. Both extracts increased the induction time compared to a control and BHA, and the stabilization factor F increased with the concentration.


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Voget K. 1995. Common Trees and Shrubs of Dryland Sudan. SOS Sahl International, U.K

Glew RS, Vander Jagt DJ, Huang YS, Chuang LT, Bosse R, Glew RH. 2004. Nutritional analysis of the edible pit of Sclerocarya birrea in the Republic of Niger (daniya, Hausa). J Food Composit. Anal. 17, 99–111.

Mizrahiy Y, Nerd A. 1996. New crops as a possible solution for the trouble Israeli export market enJanick J. (Ed.) Progress in new crops 37-45. ASHS Press, Alexandria

Mariod AA, Matthäus B, Eichner K. 2004. Fatty acid, tocopherol and sterol composition as well as oxidative stability of three unusual Sudanese oils. J Food Lipids 11, 179-189. doi:10.1111/j.1745-4522.2004.01131.x

5. Velioglu YS, Mazza G, Oomah BD. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J. Agric. Food Chem. 46, 4113-4117. doi:10.1021/jf9801973

6. Tian LL, White PJ. 1994. Antioxidant activity of oat extract in soybean and cottonseed oils. J. Am. Oil Chem. Soc. 71, 1079-1085. doi:10.1007/BF02675900

7. Duh PD, Yen YC. 1997. Antioxidant efficacy of methanolic extracts of peanut hulls in soybean and peanut oils. J. Am. Oil Chem. Soc. 74, 745-748. doi:10.1007/s11746-997-0212-z

Leonardis AD, Macciola V, Rocco AD. 2003. Oxidative stabilization of cold-pressed sunflower oil using phenolic compounds of the same seeds. J. Sci. Food Agric. 83, 523-528. doi:10.1002/jsfa.1376

Marinova EM, Yanishlieva NY. 1997. Antioxidant activity of extracts from selected species of family Lamiaceae in sunflower oil. Food Chem. 58, 245-248. doi:10.1016/S0308-8146(96)00223-3

Matthaüs B. 2002. Antioxidant activity of extracts obtained from residues of different oilseeds. J. Agric. Food Chem. 50, 3444-3452. doi:10.1021/jf011440s

Wanasundara UN, Shahidi F. 1994. Canola extract as an alternative natural antioxidant for canola oil. J. Am. Oil Chem. Soc. 71, 817-822. doi:10.1007/BF02540455

Amarowicz R, Fornal J, Karamac M, Shahidi F. 2001. Antioxidative activity of phenolic compounds from rapeseed oil cakes. J. Food Lipids 8, 65-74. doi:10.1111/j.1745-4522.2001.tb00184.x

Amarowicz R, Karamac M, Shahidi F. 2003. Antioxidant activity of phenolic fractions of lentil (Lens culinaris) . J. Food Lipids 10, 1-10. doi:10.1111/j.1745-4522.2003.tb00001.x

Braca A, Politi M, Sanogo R, Sano H, Morelli I, Pizza C, Tommasi N. 2003. Chemical composition and antioxidant activity of phenolic compounds from wild and cultivated Sclerocarya birrea (Anacardiaceae) leaves. J. Agric. Food Chem. 51, 6689-6695. doi:10.1021/jf030374m

Yanishlieva NV, Marinova EM. 1994. Antioxidative effectiveness of some natural antioxidants in sunflower oil. Z. Lebensm. Unters. Forsch. 203, 220-223. doi:10.1007/BF01192867

Gamel TH, Kiritsakis A, Petraki C. 1999. Effect of methanolic extracts of rosemary and olive oil vegetable water on the stability of olive oil and sunflower oil. Grasas Aceites 50, 345-350.

Official Methods and Recommended Practices of the American Oil Chemists Society, 4th edition by AOCS, Champaign, IL, 1993.

Silvia MT, Miller EE, Pratt DE. 1984. Chia seeds as a source of natural lipid antioxidants. J. Am. Oil Chem. Soc. 61, 928-931. doi:10.1007/BF02542169

Moure A, Franco D, Sineiro J, Dominguez H, Nunez MJ, Lema JM. 2000. Evaluation of extracts from Gevuina hulls as antioxidants. J. Agric. Food Chem. 48, 3890- 3897 doi:10.1021/jf000048w

Metrohm. Oxidationsstabilität von Ölen und Fetten - Rancimatmethode. Application Bulletin Nr. 204/1 d, 1994. Schmidt S, Niklova I, Pokorny J, Farkas P, Sekretar S. 2003. Antioxidant activity of evening primrose phenolics in sunflower and rapeseed oils. Eur. J. Lipid Sci. Technol. 105, 427-435

STATGRAPHICS®. 1985–1989. Statgraphics Statistical Graphics Systems, Version 4.0, STSC Inc. & Statistical Graphics Cooperation, Rockville, MD.




How to Cite

Mariod AA, Matthäus B, Eichner K, Hussein IH. Antioxidant activity of extracts from Sclerocarya birrea kernel oil cake. Grasas aceites [Internet]. 2006Dec.31 [cited 2024May25];57(4):361-6. Available from: