Antihypoxic and antioxidant activity of Hibiscus esculentus seeds
DOI:
https://doi.org/10.3989/gya.053809Keywords:
Antihypoxic activity, Antioxidant activity, DPPH, Flavonoids, Hibiscus esculentus, PhenolsAbstract
The antihypoxic and antioxidant activities of Hibiscus esculentus seeds were investigated employing eight in vitro assay systems. Antihypoxic activity was investigated in two models, haemic and circulatory. The effects were pronounced in both models of hypoxia. The antihypoxic effects were dose-dependent. The results indicated that the extracts have a protective effect against hypoxia induced lethality in mice. The extracts showed antioxidant activity in some models. IC50 for DPPH radical-scavenging activity was 234 ± 8.9 μg ml-1. The extracts showed weak nitric oxide-scavenging activity between 0.1 and 1.6 mg ml-1. The extracts showed weak Fe2+ chelating ability. IC50 were 150 ± 13 μg ml-1. The extracts also exhibited low antioxidant activity in the linoleic acid model but were capable of scavenging hydrogen peroxide in a concentration dependent manner. The total amount of phenolic compounds in each extract was determined as gallic acid equivalents and total flavonoid contents were calculated as quercetin equivalents from a calibration curve. Pharmacological effects may be attributed, at least in part, to the presence of phenols and flavonoids in the extracts.
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Akpanabiatu MI, Bassey NB, Udosen EO, Eyong EU. 1998. Evaluation of Some Minerals and Toxicants in Some Nigerian Soup Meals, J. food Compos Anal. 11, 292-297.
Beck T, Abdel-Rahman M, Bielenberg G, Oberpichler H, Krieglstein J. 1986. Comparative Study on the Effects of Two Extract Fractions of Ginkgo biloba on Local Cerebral Blood Flow and on Brain Energy Metabolism in the Rat Under Hypoxia, in Krieglstein J, ed. Pharmacological Study of Cerebral Ischemia. Elsevier, Amsterdam, pp. 345-350.
Calısır S, Ozcan M, Hacıseferogulları H, Yıldız MU. 2005. A study on some physico -chemical properties of Turkey okra (Hibiscus esculenta L.) seeds. J. Food Eng. 68, 73-78. doi:10.1016/j.jfoodeng.2004.05.023
Ching LS, Mohamed S. 2001. Alpha-Tocopherol Content in 62 Edible Tropical Plants. J. Agr. Food Chem. 49, 3101-3105. doi:10.1021/jf000891u PMid:11410015
Dehpour AA, Ebrahimzadeh MA, Nabavi SF, Nabavi SM. 2009. Antioxidant activity of methanol extract of Ferula assafoetida and its Essential oil composition, Grasas y Aceites 60 (4) 405-412. doi:10.3989/gya.010109
Duh PD, Tu YY, Yen GC. 1999. Antioxidant activity of water extract of harng Jyur (Chrysanthemum morifolium Ramat). Lebensm. Wiss Technol. 32, 269-277. doi:10.1006/fstl.1999.0548
Ebrahimzadeh MA, Hosseinimehr SJ, Hamidinia A, Jafari M. 2008a. Antioxidant and free radical scavenging activity of Feijoa sallowiana fruits peel and leaves. Pharmacologyonline 1, 7-14.
Ebrahimzadeh MA, Pourmorad F, Hafezi S. 2008b. Antioxidant Activities of Iranian Corn Silk. Turk. J. Biol. 32, 43-49.
Ebrahimzadeh MA, Pourmorad F, Bekhradnia AR. 2008c. Iron chelating activity screening, phenol and flavonoid content of some medicinal plants from Iran. Afr. J. of Biotech. 7 (18) 3188-3192.
Ebrahimzadeh MA, Bahramian F. 2009a. Antioxidant activity of Crataegus pentaegyna subsp. elburensis fruits extracts used in traditional medicine in Iran. P.J. Biol. Sci., 12 (5) 413-419. doi:10.3923/pjbs.2009.413.419 PMid:19579980
Ebrahimzadeh MA, Nabavi SF, Nabavi SM. 2009b. Antioxidant activities of methanol extract of Sambucus ebulus L. Flower. P.J. Biol. Sci. 12 (5) 447-450. doi:10.3923/pjbs.2009.447.450 PMid:19579986
Goli AH, Barzegar M, Sahari MA. 2005. Antioxidant activity andtotal phenolic compounds of pistachio (Pistachia vera) hull extracts. Food Chem. 92, 521-525. doi:10.1016/j.foodchem.2004.08.020
Gordon MH. 1990. The mechanism of antioxidant action in vitro. in Hudson BJF, ed. Food antioxidants. Elsevier Applied Science, London. pp. 1-18.
Hebbel RP, Leung A, Mohandas N. 1990. Oxidationinduced changes in microheological properties of the red cell membrane. Blood 76, 1015-1022. PMid:2393710
Halliwell B. 1997. Ant ioxidants: the basics- what they are and how to evaluate them. Adv. Pharmacol. 38, 3-20. doi:10.1016/S1054-3589(08)60976-X
Halliwell B, Gutteridge JMC. 1990. Role of free radicals and catalytic metal ions in human disease: an overview. Method Enzymol. 186, 1-85. doi:10.1016/0076-6879(90)86093-B
Hertog MLG., Feskens EJM., Hollman PHC., Katan MB, Kromhout D. 1993. Dietary antioxidants flavonoids and the risk of coronary heart disease: the zutphen elderly study. Lancet 342, 1007-1011. doi:10.1016/0140-6736(93)92876-U
Hosseinzadeh H, Sadati N. 2003. The Protective Effect of allium sativum L. Clove Aqueous and Methanolic Extracts Against Hypoxia-induced Lethality in Mice. Phytother Res. 17, 279-281. doi:10.1002/ptr.1114 PMid:12672162
Karcher L, Zagermann P, Krieglstein J. 1984. Effect of an extract of Ginkgo biloba on rat brain energy metabolism in hypoxia, Naynyn-Schmiedebergs Arch. Pharmacol. 327, 31-35.
Karakoltsidis PA, Constantinides SM. 1975. Okra seeds: A new protein source. J. Agr. Food Chem. 23 (6) 1204-1207. doi:10.1021/jf60202a041 PMid:1238449
Krasteva I, Nikolov I, Danchev N, Nikolov S. 2004. Phytochemical analysis of ethyl acetate extract from Astragalus corniculatus Bieb. and brain antihypoxic activity. Acta Pharm. 54, 151-156. PMid:15274758
Lee SE, Hwang HJ, Ha JS, Jeong HS, Kim JH. 2003. Screening of medicinal plant extracts for antioxidant activity. Life Sci. 73, 167-179. doi:10.1016/S0024-3205(03)00259-5
López V, Akerreta S, Casanova E, García-Mina JM, Cavero RY, Calvo MI. 2007. In vitro antioxidant and anti-rhizopus activities of Lamiaceae herbal extracts. Plant Foods Hum. Nutr. 62, 151-155. doi:10.1007/s11130-007-0056-6 PMid:17912643
McCord JM. 2000. The evolution of free radicals and oxidative stress. Am. J. Med. Sci. 108, 652-659. doi:10.1016/S0002-9343(00)00412-5
Meli R, Authore G, Di Carlo G, Capasso F. 1990. Inhibitory action of quercetin on intestinal transit in mice, Phytother. Res. 5, 201-203. doi:10.1002/ptr.2650040509
Mora A, Paya M, Rios J, Alcaraz M. 1990. Structure activity relationships of polymetoxyflavones and other flavonoids as inhibitors of non-enzymic lipid peroxidation. Biochem. Pharmacol. 40, 793-797. doi:10.1016/0006-2952(90)90317-E
Moncada A, Palmer RMJ, Higgs EA. 1991. Nitric oxide: physiology, pathophysiology and pharmacology. Pharmacol. Rev. 43, 109-142. PMid:1852778
Nabavi SM, Ebrahimzadeh MA, Nabavi SF, Hamidinia A, Bekhradnia AR. 2008a. Determination of antioxidant activity, phenol and flavonoids content of Parrotia persica Mey. Pharmacologyonline 2, 560-567.
Nabavi SM, Ebrahimzadeh MA, Nabavi SF, Jafari M. 2008b. Free radical scavenging activity and antioxidant capacity of Eryngium caucasicum Trautv and Froripia subpinata. Pharmacologyonline 3, 19-25.
Nabavi SM, Ebrahimzadeh MA, Nabavi SF, Bahramian F. 2009a. In vitro antioxidant activity of Phytolacca americana berries. Pharmacologyonline 1, 81-88.
Nabavi SM, Ebrahimzadeh MA, Nabavi SF, Fazelian M, Eslami B. 2009b. In vitro Antioxidant and Free radical scavenging activity of Diospyros lotus and Pyrus boissieriana growing in Iran. Phcog. Mag. 4(18), 122-126.
Oyelade OJ, Ade-Omowaye BIO, Adeomi VF. 2003. Influence of variety on protein, fat contents and some physical charasteristics of okra seeds. J. Food Eng. 57, 111-114. doi:10.1016/S0260-8774(02)00279-0
Purewal SS, Rhandhawa GE. 1947. Studies in Hibiscus esculentus (Ladyfinger) (okra) I. Chromosome and pollen studies. Indian J. Agr. Sci. 17, 129-136.
Reznichenko L, Amit T, Zheng H, Avramovich-Tirosh Y, Youdim MBH, Weinreb O, Mandel S. 2006. Reduction of iron-regulated amyloid precursor protein and [beta]-amyloid peptide by (-) -epigallocatechin-3-gallate in cell cultures: implications for iron chelation in Alzheimer’s disease. J. Neurochem. 97 (2) 527-536. doi:10.1111/j.1471-4159.2006.03770.x PMid:16539659
Roshtina L, Ostrovskaya R. 1981. Effect of piracetam on the body resistance to hypoxia. Farmacol. Toksikol. 44, 210-213.
Savello PA, Martins F, Hull W. 1980. Nutrition composition of okra seed meals. J. Agr. Food Chem. 28 (6) 1163-1166. doi:10.1021/jf60232a021 PMid:7451742
Sokmen M, Serkedjieva J, Daferera M, Gulluce M, Polissiou M, Tepe B et al. 2004. In vitro antioxidant, antimicrobial, and antiviral of activities of the essential oil and various extracts from herbal parts and callus cultures of Origanum acutidens. J. Agric. Food Chem. 52, 3309-3312 doi:10.1021/jf049859g PMid:15161188
Sumina E, Shugaev V, Shugaev V. 1978. The mechanism of circulatory hypoxia in acute poisoning with sodium fluoride poisoning. Farmakol. Toksikol. 41, 480-482. PMid:668876
Van Acker SABE, van Den Berg DJ, Tromp MNJL, Griffioen DH, Van Bennekom WP, van der Vijgh WJF, et al. 1996. Structural aspects of antioxidant activity of flavanoids. Free Radical Bio. Med. 20 (3) 331-342. doi:10.1016/0891-5849(95)02047-0
Yu LL. 2001. Free radical scavenging properties of conjugated linoleic acids. J. Agr. Food Chem. 49 (7) 3452-3456. doi:10.1021/jf010172v PMid:11453790
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