Separation of neutral lipid classes from bee-collected pollen by high performance liquid chromatography (HPLC)
DOI:
https://doi.org/10.3989/gya.1991.v42.i4.1233Keywords:
Bee-collected pollen, High performance liquid chromatography, Neutral lipids (separation), UV detectionAbstract
A fast method for the separation of neutral lipid classes of bee-collected pollen by isocratic HPLC is described using ultraviolet detection at 206 nm and direct injection of the sample into a silica column. The mobile phase was n-hexane-2-propanolacetic acid (100:0.5:0.1). Three fractions of neutral lipids were isolated from 35 samples: Sterol esters and other nonpolar compounds such as carotenoids and waxes; triglycerides; and fatty acids.
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Published
1991-08-30
How to Cite
1.
Muniategui S, Sancho MT, López J, Huidobro JF, Simal J. Separation of neutral lipid classes from bee-collected pollen by high performance liquid chromatography (HPLC). Grasas aceites [Internet]. 1991Aug.30 [cited 2024Apr.29];42(4):277-80. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1233
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Research
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