Production, characterization and anticancer activity of <em>Candida bombicola</em> sophorolipids by means of solid state fermentation of sunflower oil cake and soybean oil

M. M. Rashad; M. U. Nooman; M. M. Ali; A. S. Al-kashef; A. E. Mahmoud

doi:10.3989/gya.098413

Autores/as

M. M. Rashad Biochemistry Department, Division of Genetic Engineering and Biotechnology, National Research Center
M. U. Nooman Biochemistry Department, Division of Genetic Engineering and Biotechnology, National Research Center
M. M. Ali Biochemistry Department, Division of Genetic Engineering and Biotechnology, National Research Center
A. S. Al-kashef Biochemistry Department, Division of Genetic Engineering and Biotechnology, National Research Center
A. E. Mahmoud Biochemistry Department, Division of Genetic Engineering and Biotechnology, National Research Center

DOI:

https://doi.org/10.3989/gya.098413

Palabras clave:

Aceite de soja, Actividad anticancerígena, Candida bombicola, Fermentación en estado sólido, Soforolípidos, Torta de girasol

Resumen

Se estudió la producción de soforolípidos por Candida bombicola NRRL Y- 17069 cultiva con diferentes técnicas de fermentación en una mezcla de torta de girasol y aceite de soja crudo, como sustratos económicos. El rendimiento más alto (49,5 g·100 g⁻¹ de sustrato) se obtuvo por fermentación en estado sólido después de extraer con metanol (IE) seguido de acetato de etilo (EII), y de purificación parcial con hexano (EIII). También se estudió el tiempo de fermentación, considerando que el extracto IE de 12 días mostró una tensión superficial mínima (45 mN·m⁻¹) a una dilución micelar crítica (CMD) de concentración 10 %. Los soforolípidos producidos se caracterizaron y se confirmaron mediante espectroscopia FTIR y RMN de ¹H. La actividad anticancerígena de los compuestos producidos se evaluó en células MCF-7, HepG2, A549, líneas celulares de cáncer de HCT116 y los resultados revelaron que EIII y EIV (una mezcla de EI y EIII) actúan como prometedores agentes anticancerígenos en HepG2 y A549 inhibiendo las actividades de uroquinasa e histona desacetilasa.

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