Fatty acid composition of soybean/sunflower mix oil, fish oil and butterfat applying the AOCS Ce 1j-07 method with a modified temperature program

L. Masson; T. Alfaro; C. Camilo; A. Carvalho; P. Illesca; R. Torres; M. Tavares do Carmo; J. Mancini-Filho; C. Bernal

doi:10.3989/gya.0692141

Autores/as

L. Masson Centro de Investigación y Desarrollo en Grasas y Aceites. CIDGRA, Universidad de Chile - Instituto de Nutrição Josué de Castro, Universidade Federal do Rio de Janeiro
T. Alfaro Instituto Costarricense de Investigación y Enseñanza en Nutrición y Salud (INCIENSA)
C. Camilo Centro de Investigación y Desarrollo en Grasas y Aceites - CIDGRA, Universidad de Chile
A. Carvalho Instituto de Nutrição Josué de Castro, Universidade Federal do Rio de Janeiro
P. Illesca Bromatología y Nutrición, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral
R. Torres Faculdade de Ciências Farmacêuticas, Universidade de São Paulo
M. Tavares do Carmo Instituto de Nutrição Josué de Castro, Universidade Federal do Rio de Janeiro
J. Mancini-Filho Faculdade de Ciências Farmacêuticas, Universidade de São Paulo
C. Bernal Bromatología y Nutrición, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral

DOI:

https://doi.org/10.3989/gya.0692141

Palabras clave:

Aceite de pescado, Aceite de soja/girasol, Composición en ácidos grasos, Mantequilla, Método AOCS Ce 1j-07

Resumen

Métodos por cromatografía gas-líquido, AOAC 966.06 (2005), AOCS Ce 1h-05 (2005), Ce 1j-07 (2007) permiten determinar ácidos grasos (AG) en matrices grasas usando columnas capilares altamente polares y distintos programas de temperatura. No obstante, aún existen dificultades para separar ácido butírico del solvente, pares críticos de AG con polaridades similares, isómeros del ácido linoleico conjugado (CLA), AG de cadena larga poliinsaturados (LC-PUFAs). El objetivo fue mejorar el Método AOCS Ce 1j-07 aplicándolo a la composición en AG de mezcla de aceite soja/girasol, aceite de pescado, mantequilla, usando un programa de temperatura modificado, entre cinco laboratorios. El programa de temperatura elegido, si bien emplea más tiempo, permite separar completamente ácido butírico del solvente, trans-18:1 de cis-18:1, isómeros 20:1 de 18:3 n-3, 22:1 n-9 de 20:4 n-6, 20:5 n-3 de 24:0, los principales isómeros CLA. Esta propuesta permite cuantificar AG con diferentes propósitos, entre ellos, etiquetado nutricional, control de calidad e investigación.

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