Enzymatic production of sterculic acid from the novel Phoenix tree seed oil: Optimization and kinetic study

X. Hou; S. Sun

doi:10.3989/gya.0109171

Autores/as

X. Hou Lipid Technology and Engineering, School of Food Science and Engineering, Henan University of Technology https://orcid.org/0000-0003-2976-1308
S. Sun Lipid Technology and Engineering, School of Food Science and Engineering, Henan University of Technology https://orcid.org/0000-0002-3976-9250

DOI:

https://doi.org/10.3989/gya.0109171

Palabras clave:

Aceite de semilla de árbol de fénix, Ácido estercúlico, Cinética, Hidrólisis enzimática, Lipozyme TLIM, Metodología de superficie de respuesta

Resumen

El aceite de semilla del árbol fenix (Firmiana simplex) es un nuevo tipo de aceite rico en ácido estercúlico. Este es un ácido graso ciclopropeno, que se puede utilizar como inhibidor del sistema de estearoyl-CoA desaturasa y del crecimiento de los carcinomas mamarios. En este trabajo, se utilizó la hidrólisis catalizada por Lipozyme TLIM del nuevo aceite de semilla de árbol fenix para preparar ácido estercúlico. Se utilizaron GC-FID a alta temperatura y el grado de hidrólisis (DH) para monitorizar el progreso de la reacción. Los efectos de las variables de reacción de hidrólisis se evaluaron y optimizaron utilizando la metodología de superficie de respuesta. Los resultados mostraron que el ácido estercúlico puede prepararse con éxito a partir del nuevo aceite de semilla, y el efecto de las variables de reacción en la hidrólisis disminuyó en el orden de tiempo de reacción> carga enzimática> temperatura. Se pueden obtener altos rendimientos de ácidos grasos (DH, 98,2 ± 0,8%) en condiciones optimizadas (45 ºC, relación masa de agua a aceite 10: 1, carga enzimática 10% y 18 h). La ecuación de Arrhenius para la hidrólisis fue LnV₀ = 9,12-4721/T. La energía de activación fue 39,25KJ/mol. Los valores cinéticos de Vmax, K/m fueron 0,232 mol/(L∙min) y 0,084 mol/L, respectivamente.

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Citas

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