Lipase-catalyzed transesterification of epoxidized soybean oil to prepare epoxy methyl esters

W. Liu; F. Duan

doi:10.3989/gya.1103172

Autores/as

W. Liu Lipid Chemistry, College of Food Science and Technology, Henan University of Technology https://orcid.org/0000-0003-1706-1208
F. Duan Lipid Chemistry, College of Food Science and Technology, Henan University of Technology https://orcid.org/0000-0003-2772-9435

DOI:

https://doi.org/10.3989/gya.1103172

Palabras clave:

Aceite de soja epoxidado, Epoxi metil ésteres, Lipasa, Productos de base biológica, Transesterificación

Resumen

Los ésteres metílicos de aceites de soja epoxidados pueden prepararse eficientemente mediante la transesterificación del aceite de soja epoxidado (ESBO) con una menor dosificación de metanol utilizando la lipasa Novozym 435 como catalizador. Los parámetros óptimos fueron los siguientes: relación molar 5:1 de metanol:ESBO, 5% de Novozym 435 como catalizador, 45 °C durante 14 h, velocidad de agitación de 600 rpm, bajo la cual los ésteres metílicos de aceites de soja epoxidados (ESBOME) pueden obtenerse con un rendimiento del 95,7%. Durante el proceso de transesterificación enzimática, los valores de oxígeno de oxirano se mantuvieron inalterables, lo que indicó que se logra una excelente estabilidad del grupo funcional en tales condiciones de reacción suaves. Además, se examinó la reciclabilidad de la enzima inmovilizada Novozym 435 en este proceso de transesterificación y los resultados mostraron que el biocatalizador podría reutilizarse durante diez veces sin perder ninguna actividad de reacción o selectividad. Los productos finales de ESBOME se identificaron por IR y análisis de RMN. Los datos cinéticos obtenidos siguieron el modelo de mecanismo Ping-Pong Bi (V_max = 6,132 mol·L^-1min^-1, K_m,S = 0,0001 mol·L^-1, K_{m, A} = 796,148 mol·L^-1, K_{i, A} = 0,0004 mol·L^-1) con inhibición competitiva por metanol.

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Citas

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