Camellia oil saponins: Solid phase extraction and its effect on mice blood and organs

H. O.A. Ahmed; C. M. Wang; A. A. Mariod; T. A.A. Hammoda

doi:10.3989/gya.1171182

Autores/as

H. O.A. Ahmed Department of Food Science and Engineering, Institute of Food Science and Technology, Huazhong Agricultural University https://orcid.org/0000-0002-9979-0844
C. M. Wang Department of Food Science and Engineering, Institute of Food Science and Technology, Huazhong Agricultural University https://orcid.org/0000-0002-2450-3388
A. A. Mariod Department of Biology, College of Science and Arts, University of Jeddah - Indigenous Knowledge and Heritage Center, Ghibaish College of Science and Technology https://orcid.org/0000-0003-3237-7948
T. A.A. Hammoda College of Engineering and Technical Studies, Elimam Elmahadi University https://orcid.org/0000-0001-9227-7880

DOI:

https://doi.org/10.3989/gya.1171182

Palabras clave:

Aceite de semilla de camelia, Días de alimentación, Extracción en fase sólida, Saponina de té, Toxicidad

Resumen

En este estudio se optimizaron las condiciones de extracción de las saponina de té. La técnica de extracción en fase sólida se utilizó para extraer la saponina del aceite de té de camelia. Se utilizaron cuatro resinas macroporosas (DM301; NKA-9; HZ-841; S-8), dos resinas de intercambio catiónico (D001; 732), quitosán y diatomita, respectivamente, en un experimento preliminar. La resina macroporosa HZ-841 se determinó como el material adsorbente sólido óptimo. Las condiciones de extracción fueron: temperatura de 23,78 ºC, tiempo de adsorción de 5,20 horas, relación líquido:sólido: 12,54:1; tasa de adsorción prevista: 20,20%; concentración de etanol: 83,27%, el flujo de eluyente de 1,18 ml/min, relación de líquido:sólido de 21,85:1, y la velocidad de elución de la saponina del té se calculó como 59,55%. Se estudió el efecto de la saponina del aceite de semilla de té en la sangre y los órganos de los ratones (hígado, riñón, bazo y corazón). Los ratones se dividieron al azar en seis grupos y se alimentaron durante 90 días, y se registraron sus pesos todos los días. En el último día del experimento se detectaron histopatología en los elementos séricos: colesterol total (TC), triglicéridos (TG), lipoproteínas de baja y alta densidad (LDL, HDL), AST, ALT y proteína total (TP) y órganos (hígado, riñón, bazo y corazón). Los resultados del estudio demostraron que las saponinas del aceite de té aumentaron el TC, TG, LDL y HDL. Sin embargo, también en general aumentó las actividades de ALT, AST y los valores de TP en comparación con los grupos de control. Las saponinas del aceite de té no tuvieron efecto sobre la histopatología de tejidos de los órganos en comparación con los grupos de control.

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