Plasmid DNA studies in Lactobacillus plantarum strains isolated from olive fermentations: production of and immunity to plantaricin OL15 is associated to a 9.6 Kb plasmid (pOL15)
DOI:
https://doi.org/10.3989/gya.2007.v58.i2.77Keywords:
Bacteriocin, Curing agents, Lactobacillus plantarum, Olives, PlasmidAbstract
Previously 12 Lactobacillus plantarum strains were isolated from fermented olives. Among these, only L. plantarum OL15 produced bacteriocin (plantaricin OL15). In this study, the 12 strains were examined for plasmid DNA content. Of these, 9 strains have shown one to three plasmid bands ranging in size from 5.4 to 12.2 kb. L. plantarum OL15 exhibited one plasmid (9.6 kb) which was named pOL15. After curing with novobiocin and ethidium bromide, the plasmid profile analysis of non producing derivatives, showed that the 9.6 kb plasmid pOL15 harbored by the parental strain had been lost in all cases and none of them regained the ability to produce plantaricin OL15 suggesting that the production of plantaricin OL15 is plasmid linked. Plantaricin OL15 was not inactived by amylase and lipase suggesting that plantaricin OL15 activity was not dependent on the presence of either a carbohydrate or lipid moiety. Plantaricin OL15 showed activity against lactic acid bacteria of different species and also against olive spoilage and phytopathogenic bacteria, including Pseudomonas and Erwinia.
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Anderson DG, and McKay LL. 1983. Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol. 46, 549-552.
Bringel F, Frey L, Hubert JC. 1989 Characterization, cloning, curing, and distribution in lactic acid bacteria of pLP1, a plasmid from Lactobacillus plantarum CCM 1904 and its use in shuttle vector construction. Plasmid. 22, 193-202. doi:10.1016/0147-619X(89)90002-4
Casey MG, Jimeno J.1989. Lactobacillus delbruckii subsp lastis plasmids. Neth. Milk Dairy J. 43, 279-286.
De Man JC, Rogosa M, Sharpe EM. 1960. A medium for the cultivation of lactobacilli. J. Appl. Bacteriol. 23, 130-135.
Delgado S, Mayo B. 2003. Development of Lactobacillus plantarum LL441 and its plasmid-cured derivatives in cheese. J. Industr. Microbiol. Biotechnol. 30, 216-219.
Delgado A, Brito D, Fevereiro P, Marques JF. 2001. Antimicrobial activity of L. plantarum, isolated from a traditional lactic acid fermentation of table olives. Lait 81, 203-215. doi:10.1051/lait:2001124
Floriano B, Ruiz-Barba JL, Jiménez-Diáz, R.1998. Purification and genetic characterization of enterocin I from Enterococcus faecim 6T1a, a novel antilisterial plasmid-encoded bacteriocin with does not belong to the pediocin family of bacteriocins. Appl. Environ. Microbiol. 64, 4883-4890.
Franz CM, Du Toit M, Olasupo NA, Schillinger U, Holzapfel WH. 1998. Plantaricin D, a bacteriocin produced by Lactobacillus plantarum BFE 905 from ready-to-eat salad. Lett. Appl. Microbiol. 26, 231-235. doi:10.1046/j.1472-765X.1998.00332.x
Fricourt BV, Barefoot SF, Testin RF, Hayasaka SS. 1994. Detection and activity of plantaricin F, an antibacterial substance from Lactobacillus plantarum BF001 isolated from processed channel catfish. J. Food Protect. 57, 698-702.
Gonzàlez BA, Mayo P, Suarez JE. 1994. Detection, purification and partial characterization of plantaricin C, a bacteriocin produced by Lactobacillus plantarum strain of dairy origin. Appl. Environ. Microbiol. 60, 2158-2163.
Jiménez-Diáz, R, Rios-Sanchez RM, Desmazeaud M, Ruiz-Barba JL, Piard J. 1993. Plantaricins S and T, two new bacteriocins produced by Lactobacillus plantarum LPCO10 isolated from a green olive fermentation. Appl. Environ. Microbiol. 59, 1416-1424.
Kacem M. and Karam N. 2006. Microbiological study of naturally fermented Algerian green olives: isolation and identification of lactic acid bacteria and yeasts along with the effects of brine solutions obtained at the end of olive fermentation on Lactobacillus plantarum growth. Grasas Aceites 57, 292-300.
Kacem M., Zadi-Karam H. and Karam N. 2005. Detection and activity of plantaricin OL15, a bacteriocin produced by Lactobacillus plantarum isolated from Algerian fermented olives. Grasas Aceites 56, 192-197.
Kacem M, Zadi-Karam H, Karam N. 2004. Isolation of lactic acid bacteria for its possible use in the fermentation of green algerian olives. Grasas Aceites 55, 385-393.
Kacem M, Zadi-Karam H, Karam N. 2003. Lactic acid bacteria of western Algeria. Characteristics of strains isolated from raw milk and olive oil, Sci. Technol. (Algeria), 20, 45-50.
Kanatani K, Oshimura M.1994 Plasmid-associated bacteriocin production by a Lactobacillus plantarum strain. Biosci. Biotechnol. Biochem. 58, 2084-2089.
Kato T, Matsuda T, Ogawa E, Ogawa H, Kato H, Doi U and Nakamura R. 1994. Plantaricin-149, a bacteriocin produced by L. plantarum NRIC 149. J. Ferment. Bioeng. 77, 277-282. doi:10.1016/0922-338X(94)90234-8
Klaenhammer TR. 1988. Bacteriocins of lactic acid bacteria. Biochimie 70, 337-349. doi:10.1016/0300-9084(88)90206-4
Maldonado A, Ruiz-Barba, JL, Jiménez-Díaz R. 2003. Production of plantaricin NC8 by Lactobacillus plantarum NC8 is induced in the presence of different types of gram-positive bacteria. Arch. Microbiol. 181, 8-16. doi:10.1007/s00203-003-0606-8
Maldonado A, Ruiz-Barba JL, Floriano B, Jiménez-Díaz R. 2002. The locus responsible for production of plantaricin S, a class IIb bacteriocin produced by Lactobacillus plantarum LPCO10, is widely distributed among wild-type L. plantarum strains isolated from olive fermentations. Int. J. Food Microbiol. 77, 117-124. doi:10.1016/S0168-1605(02)00049-1
Mørtvedt CI, Nes TE. 1990. Plasmid-associated bacteriocin production by a Lactobacillus sake strain. J. Gener. Microbiol. 136, 1601-1607.
Ray SS, Johnson MC, Ray B. 1989. Bacteriocin plasmids of Pediococcus acidilactici. J. Ind. Microbiol. 4, 163-171. doi:10.1007/BF01574073
Ruiz-Barba J L, Piard JC, Jimenez-Diaz R. 1991. Plasmid profiles and curing of plasmids in Lactobacillus plantarum strains isolated from green olive fermentations. J. Appl. Bacteriol. 71, 417-421.
Scherwitz KM, Baldwin KA, Mckay LL. 1983. Plasmids linkage of bacteriocin-like substance in Streptococcus lactis subsp. diacetylactis strain WM4: transferability to Streptococcus lactis. Appl. Environ. Microbiol. 45, 1506-1512.
Schillinger U, Lüke FK. 1989. Antibacterial activity of Lactobacillus sake isolated from meat. Appl. Environ. Microbiol. 55, 1901-1906.
Visser R, Holzapfel WH. Bezuidenhout J. J and Kotze J. M. 1986. Antagonism of lactic acid bacteria against phytopathogenic bacteria. Appl. Environ. Microbiol. 52, 552-555.
West CA, Warner JP. 1988. Plantaricin B, a bacteriocin produced by Lactobacillus plantarum. NCDO1193. FEMS Microbiol. Lett. 49, 163-165. doi:10.1016/0378-1097(88)90452-1
Xanthopoulos V, Hatzikamari M, Adamidis T, Tsakalidou E, Tzanetakis N, Litopoulou-Tzanetaki E. 2000. Heterogeneity of Lactobacillus plantarum isolates from Feta cheese throughout ripening. J. Appl. Microbiol. 88, 1056-1064. doi:10.1046/j.1365-2672.2000.01056.x
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