Plasmid DNA studies in <i>Lactobacillus plantarum</i> strains isolated from olive fermentations: production of and immunity to plantaricin OL15 is associated to a 9.6 Kb plasmid (pOL15)

Kacem Mourad

doi:10.3989/gya.2007.v58.i2.77

Autores/as

Kacem Mourad Faculté des Sciences, Université d’Es-Senia, Oran

DOI:

https://doi.org/10.3989/gya.2007.v58.i2.77

Palabras clave:

Aceitunas, Agentes de curado, Bacteriocinas, Lactobacillus plantarum, Plásmido.

Resumen

En un estudio previo, se aislaron 12 cepas de Lactobacillus plantarum a partir de aceitunas fermentadas. Entre ellas, solo L. plantarum OL15 produjo bacteriocinas (plantaricin OL15). En este estudio, se examinó el contenido de AND plásmido en las 12 cepas citadas. Entre ellas, 9 cepas han mostrado de una a tres bandas de plásmido con tamaños en el rango de 5.4 a 12.2 kb. L. plantarum OL15 exhibió un plásmido (9.6 kb) que se denominó pOL15. Después del curado con novobiocina y bromuro de etidio, la pérdida del plásmido pOL15 asociada a la pérdida de su facultad para producir plantaricin OL15, sugiere que la producción de plantaricina OL15 está ligada al plásmido. La plantaricin OL15 no se inactivó por amilasa ni por lipasa sugiriendo que su actividad no es dependiente de la presencia de carbohidratos o lípidos. La plantaricina OL15 mostró actividad frente a bacterias lácticas de diferentes especies y también frente a residuos de aceitunas y bacterias fitopatogénicas, incluyendo Pseudomonas y Erwinia.

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Citas

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