<em>In vivo</em> correlation of olive leaves extract on some oxidative stress markers in streptozotocin-induced diabetes mellitus in rats

A. M.R. Afify; H. S. El-Beltagi; S. A. Fayed; A. E. El-Ansary

doi:10.3989/gya.1104172

Autores/as

A. M.R. Afify Biochemistry Dept, Faculty of Agriculture, Cairo University https://orcid.org/0000-0001-8096-7649
H. S. El-Beltagi Biochemistry Dept, Faculty of Agriculture, Cairo University https://orcid.org/0000-0003-4433-2034
S. A. Fayed Biochemistry Dept, Faculty of Agriculture, Cairo University https://orcid.org/0000-0002-3449-6679
A. E. El-Ansary Biochemistry Dept, Faculty of Agriculture, Cairo University https://orcid.org/0000-0003-0585-1229

DOI:

https://doi.org/10.3989/gya.1104172

Palabras clave:

Actividad de enzimas antioxidantes, Diabetes mellitus tipo dos, Estrés oxidativo, Extracto de hojas de olivo, Fenólicos Flavonoides

Resumen

La diabetes mellitus tipo dos (DM2) es una de las enfermedades más extensas en el mundo. La terapia con hierbas sigue siendo una terapia complementaria para mantener un mejor control de la glucemia y reducir las complicaciones de la diabetes. Con el fin de evaluar los efectos curativos del extracto de hojas de olivo (OLE) en ratas diabéticas inducidas por estreptozotocina (STZ), veinticuatro ratas Wistar machos adultos se dividieron en cuatro grupos iguales; control, control diabético (45 mg/ kg STZ), ratas normales tratadas con OLE (17.8 mg/kg b.wt) y ratas diabéticas tratadas con OLE (45 mg / kg STZ + 17.8 mg / kg b.wt.). El extracto OLE se investigó para determinar la actividad antioxidante in vitro usando ensayos DPPH^•. Se determinaron los fenoles, los taninos y el contenido de flavonoides. Se midió la actividad de GPX, SOD y GSH en lisado de RBC, CAT en plasma y MDA en suero. El OLE evitó la disminución de GSH y mantuvo MDA alrededor del rango normal en las ratas diabéticas tratadas. El estudio actual sugiere que OLE podría utilizarse de forma segura para mejorar la DM2 y el estrés oxidativo que le acompaña.

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