Detection of argan oil adulterated with vegetable oils: new markers


  • I. Ourrach Université Hassan II
  • M. Rada Instituto de la Grasa-CSIC
  • M. C. Pérez-Camino Instituto de la Grasa-CSIC
  • M. Benaissa Université Hassan II
  • Á. Guinda Instituto de la Grasa-CSIC



Adulteration, Argan oil, GC, HPLC, Olive oil, Sunflower oil


This work aims to contribute to controlling the authenticity of pure argan oil, a valuable Moroccan product. Fatty acids, hydrocarbon fraction, 3,5-stigmastadiene, the alkyl esters of fatty acids, chlorophyllic pigments and physical properties such as viscosity, density and refractive index were studied in order to detect the adulteration of argan oil with edible vegetable oils. The results found in this study show that 3,5-stigmastadiene, kaurene and pheophytin-a can be used as possible new markers for argan oil blends of up to 5% with refined, sunflower and virgin olive oils. Due to the similarity of the fatty acid compositions of the edible oils studied and argan oil, fatty acids can be employed as markers for the detection of argan oil adulteration at levels higher than 10%. Among the physical properties studied, the refractive index shows significant differences for sunflower oil and its blend at 10% with argan oil.


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Belarbi-Benmahdi M, Khaldi D, Beghdad C, Gouzi H, Bendimerad N. 2009. Physicochemical and nutritional study of argan oil (Argania spinosa L.) in southwestern Algeria. Pigment Resin Technol. 38 (2), 96-99.

Bortolomeazzi R, Berno P, Pizzale L, Conte LS. 2001. Sesquiterpene, alkene, and alkane hydrocarbons in virgin olive oils of different varieties and geographical origins. J. Agric. Food Chem. 49, 3278-3283. PMid: 11453763

Bueno EO, Casas JS, García AM, González LG. 2005. Discriminating power of the hydrocarbon content from virgin olive oil of extremadura cultivars. J. Am. Oil Che. Soc. 82, 1-6.

Cayuela JA, Rada M, Pérez-Camino MC, Benaissa M, Elamrani A, Guinda Á. 2008. Characterization of artisanally and semiautomatically extracted argan oils from Morocco. Eur. J. Lipid Sci. Technol. 110, 1159-1166.

Cert A, Lanzón A, Carelli AA, Albi T. 1994. Formation of 3,5-stigmastadiene in vegetable oils. Food Chem. 49, 287-293.

Charrouf Z and Guillaume D. 2008. Review. Argan oil: Occurrence, composition and impact on human health. Eur. J. Lipid Sci. Technol. 110, 632–636.

Christy AA, Kasemsumran S, Du Y, Ozak Y. 2004. The detection and quantification of adulteration in olive oil by near infrared spectroscopy and chemonetrics. Anal. Sci. 20, 935-940. PMid: 15228114

Díaz-Barradas MC, Zunzunegui M, Ain-Lhout F, Jáuregui J, Boutaleb S, Álvarez-Cansimo L, Esquivias M. 2010. Seasonal physicological responses of argania spinosa tree from mediterranean to semi-arid. Climate Plant Soil. 337, 217-231.

Dobarganes MC, Cert A, Dieffenbacher A. 1999. The determination of stigmastadienes in vegetable oils. Pure Appl. Chem. 71, 349-359. European Union Commission 1991. COMMISSION REGULATION (EEC) Nº 2568/91 ANNEX X B. Preparation of the fatty acid methyl esters from olive oil and olive-pomace oil.

Gallardo-Guerrero L, Gandul-Rojas B, Roca M, Mínguez- Mosquera MI. 2005. Effects of storage on the original pigment profile of Spanish olive oils. J. Am. Oil. Chem. Soc. 82, 33-39.

Gandul-Rojas B, Cepero MR, Mínguez-Mosquera MI. 2000. Use of chlorophyll and carotenoid pigment composition to determine authenticity of virgin olive oil. J. Am. Oil Chem. Soc. 77, 853-858.

Gonzálvez A, Armenta S, Guardia MDeLa. 2010. Adulteration detection of argan oil by inductively coupled plasma optical emission spectrometry. Food Chem. 121, 878-886.

Guinda A, Lanzón A, Albi T. 1996. Differences in hydrocarbons of virgin olive oils obtained from several olive varieties. J. Agric. Food Chem. 44, 1723-1726.

Guinda A, Rada M, Delgado T, Castellano JM. 2011. Pentacyclic triterpenic acids from arganda spinosa. Eur. J. Lipid Sci. Technol. 113, 231-237.

Harhar H, Gharby S, Kartah B, Elmonfalouti H, Guillaume D, Charrouf Z. 2011. Influence of argan kernel roasting time on virgin argan oil composition and oxidative stability. Plant Foods Hum. Nutr. 66, 163-168. PMid: 21442181

Hilali H, Charrouf Z, Soulhi A, Hachimi L, Guillaume D. 2007. Detection of argan oil adulteration using quantitative campesterol GC-analysis. J. Am. Oil Chem. Soc. 84, 761-764.

Hilali M, Charrouf Z, El Aziz Soulhi A, Hachimi L, Guillaume D. 2005. Influence of origin and extraction method on argan oil physico-chemical characteristics and composition. J. Agric. Food Chem. 53, 2081- 2087. PMid: 15769139

Hornero-Méndez D, Gandul-Rojas B, Minguez-Mosquera MI. 2005. Routine and sensitive SPE-HPLC method for quantitative determination of pheophytin a and pyropheophytin a in olive oils. Food Res International 38, 1067-1072.

Khallouki F, Younos C, Soulimani R, Oster T, Charrouf Z, Spieglehalder B, Batsch H, Owen RW. 2003. Consumption of argan oil (Morocco) with its unique profile of fatty acids, squalene, sterols, tocopherols and phenolic antioxidants should confer valuable cancer chemopreventive effects. Eur. J. Cancer Prev. 12, 67-75. PMid: 12548113

Lanzón A, Albi T, Cert A, Gracián J. 1994. The hydrocarbon fraction of virgin olive oil and changes resulting from refining. J. Am. Oil Chem. Soc. 71, 285-291.

Lanzón A, Cert A, Albi T. 1989. Detección de la presencia de aceite de oliva refinado en el aceite de oliva virgen. Grasas Aceites. 40, 385-388.

Lybbert TJ, Barrett CB, Narjisse H. 2002. Market-based conservation and local benefits: the case of argan oil in Morocco. Ecological Economics 41,125–144.

Marfil R, Cabrera-Vique C, Giménez R, Bouzas PR, Martínez O, Sánchez JA. 2008. Metal content and physicochemical parameters used as quality criteria in virgin argan oil: Influence of the extraction method. J. Agric. Food Chem 56, 7279–7284. PMid: 18662006

Moreda W, Perez-Camino MC, Cert A. 2001. Gas and liquid chromatography of hydrocarbons in edible vegetable oils. J. Chromatogr. A 936, 159-171.

Moukal A. 2004. L'arganier, argania spinosa L. (Skeels), usage therapeutique, cosmetique et alimentaire. Phytoterapie 2, 135–141.

Norme Marocaine. PNM 08.5.090. 2003. Corps gras d'origines animale et végétale. Huiles d'argane spécifications. Ministry of Industry, Trade, Energy and Mines. Rabat, 2003.

Perez-Camino MC, Cert A, Romero-Segura A, Cert-Trujillo R, Moreda W. 2008. Alkyl esters of fatty acids a useful tool to detect soft deodorized olive oils. J. Agric. Food Chem. 56, 6740-6744. PMid: 18642925

Perez-Camino MC, Moreda W, Mateos R, Cert A. 2002. Determination of esters of fatty acids with low molecular weight alcohols in olive oils. J. Agric. Food Chem 50, 4721-4725. PMid: 12137504

Rezanka T, Rezanková H. 1999. Characterization of fatty acids and triacylglycerols in vegetable oils by gas chromatography and statistical analysis. Analytica Chimica Acta. 398, 253-261.

Ruiz-Méndez MV, Ramos-Hinojosa ÁE. 2003. Fatty acid esters with short-chain alcohols in two-phase olive pomace oils. Eur J Lipid Sci Technol 105, 346–350.

Webster L, Simpson P, Shanks AM, Moffat CF. 2000. The authentication of olive oil on the basis of hydrocarbon concentration and composition. The Analyst 125, 97-104.

Yaghmur A, Aserin A, Mizrahi Y, Nerd A, Garti N. 2001. Evaluation of argan oil for deep-fat frying. Lebensm. Wiss. Technol .34, 124-130.

Zabaras D. 2010. Olive oil adulteration with hazelnut oil and analytical approaches for its detection, en Victor R. Preedy and Ronald Ross (Eds.) Olives and Olive Oil in Health and Disease Prevention. Elsevier Inc., págs. 441-450.

Zougagh M, Salghi R, Dhair S, Rios A. 2011. Nanoparticle-based assay for the detection of virgin argan oil adulteration and its rapid quality evaluation. Anal. Bioanal Chem. 399, 2395-2405. PMid: 21221541




How to Cite

Ourrach I, Rada M, Pérez-Camino MC, Benaissa M, Guinda Á. Detection of argan oil adulterated with vegetable oils: new markers. grasasaceites [Internet]. 2012Dec.30 [cited 2021May7];63(4):355-64. Available from:




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