Evaluation of the oil Produced from lettuce crop cultivated under three irrigation conditions


  • M. Hassan El-Mallah Fats and Oil Department, National Research Centre
  • S. M. El-Shami Fats and Oil Department, National Research Centre




Fatty acids, Lettuce, Lipids, Sterols, Tocopherols, Triacylglycerols


Three oil lettuce seed samples (lactuca Sativa LS10, LS20, LS30) were cultivated under three irrigation conditions (well, normal and water deficient conditions, after 10, 20 and 30 days respectively) to evaluate their oils and to see to what extent the oil lettuce plant resists draught conditions. The oils extracted from the three seed samples were evaluated by determining eight lipid profiles using HPLC in conjunction with capillary GLC. Lettuce seed oils are characterized by high contents of linoleic and oleic acids. Of the triacyl glycerols determined, those containing linoleyl and oleyl acyles are the major ones. The whole sterol profiles include β-sitosterol (as major component) followed by 7-stigmasterol, campesterol and 5-stigmasterol, which were found in all the lettuce seed oil samples but with slight differences. Furthermore, sterol patterns of the free and acylated sterols, free and acylated sterylglycosides were determined. It was found that LS30 oil has the highest tocopherol content and α-tocopherol is the only constituent in all the lettuce seed oil samples. On the other hand, the 2-position in the lettuce seed oil samples is mainly acylated by unsaturated fatty acids (98.6%) namely, oleic and linoleic acids. According to these results, it can be concluded that irrigation conditions do not affect the lipid constituents of the oil and the oil lettuce plant resists draught and its lipid profiles are in agreement with those of conventional vegetable oils.


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Christie WW. 1973. The preparation of volatile derivatives of lipids. in Lipid Analysis. Chapter 4, Pergamon press, pp.85-102.

Clark J. 1996. Tocopherols and sterols from soybean. Lipid Technol. 8, 111-114.

El-Din Ahmed A, EL- Ghazouly Maged G, Abou Donia Amin H. 1987. Phytochemical Study of Lactuca Salinga L. Growing in Egypt. Alexandria J. Pharm. Sci. 1, 5-7.

El-Hamdy AH, Perkins EG 1981. High performance reversed phase chromatography of natural triglyceride mixtures: critical pair separation. J. Am. Oil Chem. Soc. 58, 867-872. http://dx.doi.org/10.1007/BF02672960

El-Mallah HM, El-Shami SM, Hassanein Minar MM. 2003. Studies of Lipid Profiles of Vitis Vinifera (Grape) and Lactuca Scariola (Oil Lettuce) Seed Oils in Comparison with those of Soybean Oil. Mansuora University J. Agric. Sci. 28, 3179-3187.

El-Mallah MH, Murui T, El-Shami SM. 1999. New trends in determining the authenticity of corn oil. Grasas Aceites. 50, 7-15. http://dx.doi.org/10.3989/gya.1999.v50.i1.629

El-Mallah MH, Murui T, El-Shami SM. 1994. Detailed studies on seed oil of silicornia SOS-7 cultivated at the Egyptain border of Red Sea. Grasas Aceites. 45, 385-389. http://dx.doi.org/10.3989/gya.1994.v45.i6.1036

El-Shami S, Hassanein MM, Murui T, El-Mallah MH. 1994. Studies of changes in patterns of fatty acids, sterols and tocopherols of oil during seed maturation part 1: sunflower seeds. Grasas Aceites 45, 227-231. http://dx.doi.org/10.3989/gya.1994.v45.i4.999

Gunstone FD, Harwood LJ, Padly BF. 1986. The Lipid Hand Book. Chapman& Hall. Cambridge. P. 571.

Kamal-Eldein A, Appeleqvist AL. 1996. The chemistry and antioxidant properties of tocopherols and tocotrienols. Lipids 31, 671-701. http://dx.doi.org/10.1007/BF02522884

Ling WH, Jones PJ.1995. Dietary phytosterols: a review of metabolism, benefits and side effects. Life Sci. 57, 195-206. http://dx.doi.org/10.1016/0024-3205(95)00263-6

McClave JT, Benson, PG.1991. Statistics for Business and Economics, Max Well Macmillian International Editions. Dellen Publishing Co. USA, pp. 272-295.

Mekki BB, El- Kholy MA, Mohamed EM. 1998. Effect of Folair Application of Potassin-P and Plant Spacing on Seed Yield, Oil and Fatty Acid Contents of Prickly Oil Lettuce (Lactuca Scariola L.) 8th Con.Agron. Suez Canal Univ. Ismailia, Egypt 28-29 Nov.

Mekki BB, Orabi SA. 2007. Risponse of Prickly Oil Lettuce (Lactuca Scariola L.) to Uniconazole and Irrigation with Diluted Seawater. Am. Eurasian Agric. Environ. Sci. 2, 611-618.

Moreau RA, Whitaker BD, Hicks KB. 2002. Phytosterols and their conjugates in foods: Structural diversity quantitative analysis, and health- promoting uses. Prog. Lipid Res. 41, 457-500. http://dx.doi.org/10.1016/S0163-7827(02)00006-1

Murui T, Wanaka, K, Kuriyama K. 1993. Determination of sterylglycosides as their 1- anthroylnitrile derivatives. Biosci. Biotech. Biochem. 25, 614-617. http://dx.doi.org/10.1271/bbb.57.614

Murui T, Yoshikawa M, Takeuchi H, Fujii S, Mizobuchi H.1994. Effect of sterylglycosides from soybean on lipid indices in the plasma, liver, and feces of rats. Biosci. Biotech. Biochem. 58, 494-497. http://dx.doi.org/10.1271/bbb.58.494

Official and Tentative Methods of the American Oil chemists society.1980. 3rd ed. AOCS, Chicago, Illinois. Osman F, Ashourm AE, Gad AM 1973. Glyceride structure of Egyptian vegetable oils. Planta Med. 24, 53-60.

Rafi A, Tufail MQ. 1991. Studies of Lactuca Scariola Seed Oil. Sci.Int. (Lahore) 3, 67-68.

Wanaka K, Murui T. 1992. Determination of sterols in edible oils and fats by HPLC with fluorescence labeling. Yukagaku, 40, 306-311.




How to Cite

Hassan El-Mallah M, El-Shami SM. Evaluation of the oil Produced from lettuce crop cultivated under three irrigation conditions. grasasaceites [Internet]. 2012Dec.30 [cited 2022Dec.6];63(4):423-31. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1396