Enzymatic modification of phosphatidylcholine with n-3 PUFA from silkworm oil fatty acids


  • Shiva Shanker Kaki Centre for Lipid Research, CSIR-Indian Institute of Chemical Technology
  • T. Ravinder Centre for Lipid Research, CSIR-Indian Institute of Chemical Technology
  • B. Ashwini Centre for Lipid Research, CSIR-Indian Institute of Chemical Technology
  • B. V.S.K. Rao Centre for Lipid Research, CSIR-Indian Institute of Chemical Technology
  • R. B.N. Prasad Centre for Lipid Research, CSIR-Indian Institute of Chemical Technology




α-linolenic acid, Egg phosphatidylcholine, Lipase, Nutraceuticals, Silkworm oil


α-Linolenic acid (ALA) containing phosphatidylcholine (PC) was prepared by an enzymatic method employing natural substrates comprising of egg and eri silkworm oil. Eri silkworm oil extracted from eri pupae was saponified to obtain the fatty acid mixture which was further subjected to urea complexation to obtain an ALA rich fraction with a purity of about 93%. Transesterification of PC with the ALA rich fraction with three immobilized lipases namely Lipozyme TL IM, Lipozyme RM IM and lipase from Candida Antarcticaz showed that only the lipase from Candida antarctica was successful for the incorporation of ALA into egg yolk PC. It was found that ALA was incorporated by up to 27% in the sn-1 position of egg PC and the positional distribution analysis of fatty acids in the modified PC showed that the sn-1 position was found to contain about 59% ALA.


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How to Cite

Shanker Kaki S, Ravinder T, Ashwini B, Rao BV, Prasad RB. Enzymatic modification of phosphatidylcholine with n-3 PUFA from silkworm oil fatty acids. Grasas aceites [Internet]. 2014Jun.30 [cited 2024May25];65(2):e021. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1487




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