Kinetics of enzymatic hydrolysis of methyl ricinoleate
Keywords:Hydrolysis, Kinetics, Methyl ricinoleate, Ricinoleic acid
Ricinoleic acid is an unsaturated hydroxy fatty acid that naturally occurs in castor oil in proportions of up to 85–90%. Ricinoleic acid is a potential raw material and finds several applications in coatings, lubricant formulations and pharmaceutical areas. Enzymatic hydrolysis of castor oil is preferred over conventional hydrolysis for the preparation of ricinoleic acid to avoid estolide formation. A kinetics analysis of the enzymatic hydrolysis of Methyl Ricinoleate in the presence of Candida antarctica Lipase B was carried out in this study by varying reaction temperature (40–60 °C) and enzyme concentration (2–5%). The optimal conditions were found to be 6 h reaction time, temperature 60°C, buffer to methyl ricinoleate ratio 2:1(v/w) and 4% enzyme concentration to achieve a maximum conversion of 98.5%. A first order reversible reaction kinetic model was proposed to describe this reaction and a good agreement was observed between the experimental data and the model values. The effect of temperature on the forward reaction rate constant was determined by fitting data to the Arrhenius equation. The activation energy for forward reaction was found to be 14.69 KJ·mol−1.
AOCS. 2004a. Acid value. Cd 3d-63. In: Firestone D (ed) Official methods and recommended practices of the American Oil Chemists' society, 4th edn. American Oil Chemists' Society Press, Champaigne.
AOCS. 2004b. Saponification value. Cd 3-25. In: Firestone D (ed) Official methods and recommended practices of the American Oil Chemists' society, 4th edn. American Oil Chemists' Society Press, Champaigne.
Berdeaux O, Christie WW, Gunstone FD, Sebedio JL. 1997. Large- Scale Synthesis of Methyl cis-9,trans-11- Octadecadienoate from Methyl Ricinoleate. J. Am. Oil Chem. Soc. 74, 1011–1015. http://dx.doi.org/10.1007/s11746-997-0018-z
Gamayurova VS, Zinoveva ME, Tran HTT. 2013. Features of the enzymatic hydrolysis of castor oil. Catalysis Indust. 5, 269–273. http://dx.doi.org/10.1134/S2070050413030045
Goswami D, Basu JK, De S. 2013. Lipase applications in oil hydrolysis with a case study on castor oil: a review. Crit. Rev. Biotechnol. 33, 81–96. http://dx.doi.org/10.3109/07388551.2012.672319 PMid:22676042
Goswami D, Sen R, Basu JK, De S. 2009a. Maximization of bioconversion of castor oil into ricinoleic acid by response surface methodology. Bioresour. Technol. 100, 4067–4073. http://dx.doi.org/10.1016/j.biortech.2008.11.040 PMid:19419859
Goswami D, Basu JK, De S. 2009b. Optimization of process variables in castor oil hydrolysis by candida rugosa lipase with buffer as dispersion media. Biotechnol. Bioprocess Eng. 14, 220–224. http://dx.doi.org/10.1007/s12257-008-0123-3
Knezevic ZD, Siler-Marinkovic SS, Mojovic LV. 1998. Kinetics of lipase-catalyzed hydrolysis of palm oil in lecithin/ izooctane reversed micelles. Appl. Microbiol. Biotechnol. 49, 267–271. http://dx.doi.org/10.1007/s002530051167
Kulkarni SR, Pandit AB. 2005. Enzymatic hydrolysis of castor oil: An approach for rate enhancement and enzyme economy. Indian J. Biotechnol. 4, 241–245.
Lakshminarayana G, Subbarao R, Sita Rama Sastry Y, Kale V, Chandrasekhara Rao T, Gangadhar A. 1984. High pressure splitting of castor oil. J. Am. Oil Chem. Soc. 61, 1204–1206. http://dx.doi.org/10.1007/BF02636251
Neeharika TSVR, Lokesh P, Prasanna Rani KN, Prasad RBN. 2014. Optimization of Enzymatic Hydrolysis of Enriched Castor Oil Methyl Ester using Response Surface Methodology. J. Lipid Sci. Technol. 46, 8–14.
Patel BP, Patel HS, Patel SR. 2004. Modified castor oil as an epoxy resin curing agent. E-J Chem. 1, 11–16. http://dx.doi.org/10.1155/2004/412906
Puthli MS, Rathod VK, Pandit AB. 2006. Enzymatic hydrolysis of castor oil: process intensification studies. Biochem. Eng. J. 31, 31–41. http://dx.doi.org/10.1016/j.bej.2006.05.017
Rao KVSA, Paulose MM, Lakshminarayana G. 1990. In situ lipolysis of castor oil in homogenised castor seeds. Biotechnol. Lett. 12, 377–380. http://dx.doi.org/10.1007/BF01024435
Rao KVSA, Paulose MM. 1992. A process for splitting of castor oil at ambient temperature using homogenised castor seed as lipase source. Res. Ind. 37, 36–37.
Rao KVSA, Vijayalakshmi P, Prasad RBN. A process for the enrichment of methyl ricinoleate from castor oil methyl esters by liquid liquid extraction. Patent no. WO 2009/109985.
Rathod VK, Pandit AB. 2009. Effect of various additives on enzymatic hydrolysis of castor oil. Biochem. Eng. J. 47, 93–99. http://dx.doi.org/10.1016/j.bej.2009.07.008
Sharon C, Nakazato M, Ogawa HI, Kato YM. 1999. Bioreactor operated production of lipase: castor oil hydrolysis using partially-purified lipase. Indian J. Exp. Biol. 37, 481–486. PMid:10492620
Yamamoto K, Fujiwaran N. 1995. The hydrolysis of castor oil using a lipase from Pseudomonas sp. f-B-24: positional and substrate specificity of the enzyme and optimum reaction conditions. Biosci. Biotech. Biochem. 59, 1262–1266. http://dx.doi.org/10.1271/bbb.59.1262
Yang JW, Jeon GJ, Hur BK. 1999. Hydrolysis of castor oil with lipases and organic solvents. Korean J. Biotechnol. Bioeng. 14, 696–701.
How to Cite
Copyright (c) 2015 Consejo Superior de Investigaciones Científicas (CSIC)
This work is licensed under a Creative Commons Attribution 4.0 International License.© CSIC. Manuscripts published in both the printed and online versions of this Journal are the property of Consejo Superior de Investigaciones Científicas, and quoting this source is a requirement for any partial or full reproduction.
All contents of this electronic edition, except where otherwise noted, are distributed under a “Creative Commons Attribution 4.0 International” (CC BY 4.0) License. You may read here the basic information and the legal text of the license. The indication of the CC BY 4.0 License must be expressly stated in this way when necessary.
Self-archiving in repositories, personal webpages or similar, of any version other than the published by the Editor, is not allowed.