Characterization of high-oleic peanut natural mutants derived from an intersectional cross
Keywords:Arachis, FAD2A, FAD2B, GC, High oleate, Intersectional hybrid, NIR
As compared with its normal oleate counterpart, high oleate peanuts have better storage quality and several health benefits, and are therefore preferred by peanut shellers and consumers. High oleate has now become one of the main breeding objectives of peanuts. Thus far, over 50 high oleate peanut cultivars have been registered. Yet high oleate peanut breeding relies heavily on a limited number of high oleate genotypes. In this paper, we reported, for the first time, high peanut oleate natural mutants with large seeds derived from an intersectional cross, which were identified with near infra-red spectroscopy and confirmed by gas chromatography. Sequencing of FAD2 from the high-oleic hybrids along with their normal oleate parents indicated that a 448G >A mutation in FAD2A coupled with a 441_442ins A or G in FAD2B together caused high oleate phenotypes in these peanut hybrids.
Bruner AC, Jung S, Abbott AG, Powellm GL. 2001. The naturally occurring high oleate oil character in some peanut varieties results from reduced oleoyl-PC desaturase activity from mutation of aspartate 150 to asparagine. Crop Sci. 41, 522–526. http://dx.doi.org/10.2135/cropsci2001.412522x
Davis JP, Sweigart DS, Price KM, Dean LL, Sanders TH. 2013. Refractive index and density measurements of peanut oil for determining oleic and linoleic acid contents. J. AOCS. 90, 199–206. http://dx.doi.org/10.1007/s11746-012-2153-4
Jiang HF, Ren XP, Huang JQ, Lei Y, Liao BS. 2009. Genetic variation of fatty acid components in Arachis species and development of interspecific hybrids with high oleic and low palmitic acids. Acta Agron. Sinica. 35, 25–32. http://dx.doi.org/10.1016/S1875-2780(08)60053-X
López Y, Smith OD, Senseman SA, Rooney WL. 2001. Genetic factors influencing high oleic acid content in Spanish market-type peanut cultivars. Crop Sci. 41, 51–56. http://dx.doi.org/10.2135/cropsci2001.41151x
Norden AJ, Gorbet DW, Knauft DA, Young CT. 1987. Variability in oil quality among peanut genotypes in the Florida breeding program. Peanut Sci. 14, 7–11. http://dx.doi.org/10.3146/i0095-3679-14-1-3
Patel M, Jung S, Moore K, Powell G, Ainsworth C, Abbott A. 2004. High-oleate peanut mutants result from a MITE insertion into the FAD2 gene. Theor. Appl. Genet. 108, 1492–502. http://dx.doi.org/10.1007/s00122-004-1590-3 PMid:14968307
Tang YY, Wang XZ, Wu Q, Sun QX, Tang RH, Gao HY, Wang CT. 2013. Evaluation of wild peanut species for fatty acid composition. J. Today's Biol. Sci. Res. Rev. 2, 21–28.
Wang CT, Wang XZ, Li GJ, Zhang JC, Yu SL. 2011. Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content. Electorn. J. Biotechn. 14(2).
Wang CT, Yu HT, Tang YY, Wang XZ, Wu Q, Gao HY, Hu DQ, Song GS, Chen JH, Yu SL. 2012. Production of peanut hybrid seeds in an intersectional cross through postpollination treatment of flower bases with plant growth regulators. Plant Growth Regul. 68, 511–515. http://dx.doi.org/10.1007/s10725-012-9726-y
Wang CT, Zhang JC, Tang YY, Guan SY, Wang XZ, Wu Q, Shan L, Zhu LG, Su JW, Yu ST. (Ed.). 2013. Genetic Improvement of Peanut. Shanghai Science & Technology Press. Shanghai, China.
Wang CT, Wang XZ, Tang YY, Wu Q, Xu JZ, Hu DQ, Qu B. 2014. Predicting main fatty acids, oil and protein content in intact single seeds of groundnut by near infrared spectroscopy. Advd. Mater. Res. 860–863, 490–496. http://dx.doi.org/10.4028/www.scientific.net/AMR.1039.490
Wang CT, Wang XZ, Tang YY, Wu Q, Sun QX, Gong QX, Yang Z, Hu DQ, Xu ZJ, Ni WL, Zhai XL, Gao HY, Chen RH, Wang XL, Yu ST, Qian L. 2014. Chapter 6. Genetic improvement in oleate content in peanuts. In Richard W. Cook (Ed.) Peanuts: Production, Nutritional Content and Health Implications. Nova Science Publisher. pp. 95–140.
Yang CD, Guan SY, Tang YY, Wang XZ, Wu Q, Gong QX, Wang CT. 2012. Rapid non-destructive determination of fatty acids in single groundnut seeds by gas chromatography. J. Peanut Sci. 41, 21–26.
Yu S, Pan L, Yang Q, Min P, Ren Z, Zhang H. 2008. Comparison of the Δ12 fatty acid desaturase gene between high-oleic and normal-oleic peanut genotypes. J. Genet. Genomics. 35, 679–685. http://dx.doi.org/10.1016/S1673-8527(08)60090-9
Yu ST, Wang CT, Yu SL, Wang XZ, Tang YY, Chen DX, Zhang JC. 2010. Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain Reaction. Electorn. J. Biotechn. 13(4).
Zhang JC, Wang CT, Wang XZ, Tang YY, Cui FG, Chen DX. 2009. Quality analysis of 27 peanut lines. In China Crops Society (Ed.) Proceedings of Annual Meeting of China Crops Society, Guangzhou. p. 152. PMCid:PMC2783902
How to Cite
Copyright (c) 2015 Consejo Superior de Investigaciones Científicas (CSIC)
This work is licensed under a Creative Commons Attribution 4.0 International License.© CSIC. Manuscripts published in both the printed and online versions of this Journal are the property of Consejo Superior de Investigaciones Científicas, and quoting this source is a requirement for any partial or full reproduction.
All contents of this electronic edition, except where otherwise noted, are distributed under a “Creative Commons Attribution 4.0 International” (CC BY 4.0) License. You may read here the basic information and the legal text of the license. The indication of the CC BY 4.0 License must be expressly stated in this way when necessary.
Self-archiving in repositories, personal webpages or similar, of any version other than the published by the Editor, is not allowed.