Optimum conditions for enzymatic degradation of some oilseed proteins

Authors

  • F. S. Taha Fats and Oils Department
  • M. A. Ibrahim Fats and Oils Department
  • E. A. El-Zanaty Pilot Plant and Chemical Engineering Department. National Research Center

DOI:

https://doi.org/10.3989/gya.2002.v53.i3.316

Keywords:

Bromelain, Enzymatic hydrolysis, Oilseeds, Papain

Abstract


Soybean, sesame seed, and rice bran meal proteins were hydrolyzed with two enzymes, namely, papain and bromelain. Experiments were carried out to elucidate the optimum condition for each enzyme when acting on each substrate seperately. Results revealed that the highest relative activities for papain were achieved with E/S 0.06 , 0.29, 0.19 and pH 7.2, 7.0, 7.0 for soybean, sesame,and rice bran meal proteins, respectively. Optimum temperature for papain while hydrolysing the three substrates was 50 ºC. When using bromelain optimum E/S resulting in highest relative activities were 0.067, 0.058 and 0.21 for soybean, sesame,and rice bran meal protein, respectively. Optimum pH was 6.0 and optimum temperature was 45 ºC for bromelain when hydrolysing the protein of the three substrates. A numerical correlation of enzymatic behaviour for the different substrates was calculated.

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Published

2002-09-30

How to Cite

1.
Taha FS, Ibrahim MA, El-Zanaty EA. Optimum conditions for enzymatic degradation of some oilseed proteins. grasasaceites [Internet]. 2002Sep.30 [cited 2022Dec.7];53(3):267-72. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/316

Issue

Section

Research