Kinetic study of soybean pure powder lecithin hydrolysis using immobilized phospholipase A2

Authors

  • B. Maroto Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba
  • C. Camusso Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba
  • N. Zaritzky Depto de Ingeniería Química. Facultad de Ingeniería UNLP y CIDCA. Facultad de Ciencias Exactas, Universidad Nacional de La Plata

DOI:

https://doi.org/10.3989/gya.2001.v52.i1.393

Keywords:

A<sub>2</sub> Phospholipase enzyme, Enzymatic hydrolysis, Immobilized enzymes, Soybean lecithin

Abstract


Immobilized A2 phospholipase enzyme promotes the hydrolytic reaction of pure powder soybean lecithin releasing a mole of fatty acid from C-2 position. The main purpose of this paper was to determine the kinetic parameters of this reaction when the enzyme was adsorbed on alumina or DEAE-sephadex. The best conditions for the reaction were: temperature: 45-48ºC, Ca ions concentration: 6mM, pH: 8,65. Tested conditions for substrate concentration were: 6,3; 12,7; 19 and 25 mM working in a batch type reactor and with the immobilized enzyme. The incubating time did not change the enzymatic activity. The hydrolytic activity of alumina or DEAE-sephadex adsorbed A2 phospholipase enzyme was lower than of the soluble enzyme because the intrinsic properties are modified by immobilization. For substrate concentrations ranging between 6 and 19 mM first order kinetic velocity constants were k = 9, 88. 10-2 min-1 and k = 1,766. 10-1 min-1 for alumina and DEAEsephadex respectively. For the same supports but at higher substrate concentration (25mM) the zero order kinetic velocity constants were k= 1,62. 10-3 mol.l-1min-1 (alumina) and k = 3,58. 10-3 mol.l-1 min-1 (DEAE-sephadex).

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Published

2001-02-28

How to Cite

1.
Maroto B, Camusso C, Zaritzky N. Kinetic study of soybean pure powder lecithin hydrolysis using immobilized phospholipase A2. grasasaceites [Internet]. 2001Feb.28 [cited 2023Feb.6];52(1):33-7. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/393

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Section

Research