Phospholipase A2 enzyme immobilization
DOI:
https://doi.org/10.3989/gya.2000.v51.i3.471Keywords:
Immobilization, Phospholipase A2, SupportAbstract
A series of several supports were tested for phospholipase A2 enzyme (Lecitase 100 S- NOVO) immobilization. This system would find a number of industrial applications such as the selective hydrolysis of soybean lecithin to obtain lysophospholipids (modified lecithins) of great importance in pharmaceuticals and bread making. Successful results have been obtained from the immobilization of Phospholipase A2 on Alumina, Silica gel, Amberlite IRA-400S, Dowex and Sephadex A-25 via adsorption (the results were in the range 15-30 % of enzyme adsorption). The immobilization reaction was carried out at ambient temperature, with slow magnetic agitation such that the enzyme and 50 mg support were present in the total reaction volume of 5 ml in Tris-ClH buffer (pH 8.65). Several enzyme/supports ratio and the adsorption percentage with respect to pH and temperature and time were investigated. We have compared the behaviour of the phospholipase A2 in soluble form and immobilized form toward pure powder soybean lecithin as substrate.
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