Effects of germination on chemical composition and antioxidant activity of flaxseed (Linum usitatissimum L) oil
DOI:
https://doi.org/10.3989/gya.0463141Keywords:
Antioxidant activity, Germination, Oil, Oil stability, Physicochemical characteristicsAbstract
The present study was carried out to determine the changes in proximate composition and physicochemical characteristics of flaxseed during germination. Flaxseed was germinated for 4 days and observations were taken every day throughout the study. Changes in the seed reserve and antioxidant activity were determined during germination. The oil content of the cultivar decreased from 35.10 to 27.22%. During the germination period, the total protein content increased to 23.84%. Germinated flaxseed showed significantly higher unsaturated as compared to saturated fatty acid ratios and higher calculated oxidizability (Cox) values. The Saponification value ranged from 182 to 192 mg KOH·g–1 oil during germination. The highest peroxide value (2.4 mequiv O2·kg−1 oil) was observed at the end of germination. The unsaponifiable contents ranged from 1.62 to 1.18%. The oxidation value of the oil samples were statistically in the same range (4.1–6.4%). After 4 days of germination, oil stability was reduced to 1.0 h. The increase in ascorbic acid content was steady. Total phenolic acid contents differed significantly. The greatest concentration was detected in non germinated flaxseed oil. Germinated Flaxssed oil showed an important free radical scavenging activity towards 1-1-diphenyl-2-picrylhydrazyl (DPPH) free radicals.
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Aminul Islam AKM, Yaakob Z, Anuar N, Primandari SRP, Osman M. 2012. Physiochemical Properties of Jatropha curcas Seed Oil from Different Origins and Candidate Plus Plants (CPPs). J. Am. Oil. Chem. Soc. 89, 293–300. http://dx.doi.org/10.1007/s11746-011-1908-7
Anwar F, Zreen Z, Sultana B, Jamil A. 2013. Enzyme-aided cold pressing of flaxseed (Linum usitatissimum L.): Enhancement in yield, quality and phenolics of the oil. Grasas Aceites. 64, 463–471. http://dx.doi.org/10.3989/gya.132212
AOAC. 1990. Official Methods of Analysis. 15th Edn, Association of Official Analytical Chemists, Washington DC.
Azhari S, Xu YS, Jiang QX, Xia WS. 2014. Physicochemical properties and chemical composition of Seinat (Cucumis melo var. tibish) seed oil and its antioxidant activity. Grasas Aceites. 65, 1–9. http://dx.doi.org/10.3989/gya.074913
Bamgboye AI, Hansen AC. 2008. Prediction of cetane number of biodiesel fuel from the fatty acid methyl ester (FAME) composition. Int. Agrophys. 22, 21–29.
Bewley JD, Black M. 1994. Seeds: Physiology of Development and Germination, Plenum Press, New York. http://dx.doi.org/10.1007/978-1-4899-1002-8
Bose PK. 2009. Empirical approach for predicting the cetane number of biodiesel. Int. J. Automot. Technol. 10, 421–429. http://dx.doi.org/10.1007/s12239-009-0048-7
Choo W-S, Birch J, Dufour J-P. 2007. Physicochemical and quality characteristics of cold-pressed flaxseed oils. J. Food Compos Anal. 20, 202–211. http://dx.doi.org/10.1016/j.jfca.2006.12.002
Codex Alimentarius Commission, Codex Stan 19. Edible fats and oils not covered by individual standards, http://www.codexalimentarius.net/web/standard_list.do?lang=en (accessed Jan. 2006).
Demirbas A. 1998. Fuel properties and calculation of higher heating values of vegetable oils. Fuel. 77, 1117–1120. http://dx.doi.org/10.1016/S0016-2361(97)00289-5
Dieffenbacher A, Pocklington WD. 1987. Standard methods for the analysis of oils, fats and derivatives. International Union of Pure and Applied Chemistry/Blackwell, Oxford.
Fatemi SH, Hammond EG. 1980. Analysis of oleate, linoleate and linolenate hydroperoxides in oxidized ester mixtures. Lipids. 15, 379–385. http://dx.doi.org/10.1007/BF02533555
Gorinstein S, Cvikrova M, Machackova I, Haruenkit R, Park YS, Jung ST. 2004. Characterization of antioxidant compounds in Jaffa sweeties and white grapefruits. Food Chem. 84, 503–510. http://dx.doi.org/10.1016/S0308-8146(03)00127-4
Graham IA. Seed storage oil mobilization. 2008. Annu Rev Plant Biol. 59, 115–142. http://dx.doi.org/10.1146/annurev.arplant.59.032607.092938
Gujral HS, M Angurala, P Sharma, J Singh. 2011. Phenolic content and antioxidant activity of germinated and cooked pulses. Int. J. Food. Prop. 14, 1366–1374.
Gutfinger T. 1981. Polyphenols in olive oils. J. Am. Oil Chem. Soc. 58, 966–968. http://dx.doi.org/10.1007/BF02659771
Gutiérrez F. 1989. Determination of virgin olive oils stability: Comparization between activated oxygen (AOM) and Rancimat Methods. Grasas Aceites 40, 1–5.
Hahm TS, Park SJ, Lo YM. 2009. Effects of germination on chemical composition and functional properties of sesame (Sesamum indicum L.) seeds. Biores Technol. 100, 1643–1647. http://dx.doi.org/10.1016/j.biortech.2008.09.034
Lechvallier D. 1966. The lipids of Lemnaceae, analysis of fatty acids of lipids of fronds of Spirodela polyrhiza. C. R. Acad. Sci. 263, 1848–1852.
Lee CK, Karunanithy R. 1990. Effects of germination in the chemical composition of glycine and phaseolus beans. J. Sci. Food Agr. 51, 437–445. http://dx.doi.org/10.1002/jsfa.2740510403
Linkies A, Graeber K, Knight C, Gerhard LM. 2010. The evolution of seeds. New Phytol. 186, 817–831. http://dx.doi.org/10.1111/j.1469-8137.2010.03249.x
List GR, Evans CD, Kwolek WF, Warner K, Boundy BK, Cowan JC. 1974. Oxidation and quality of soybean oil: a preliminary study of the anisidine test. J. Am. Oil Chem. Soc. 51, 17–21. http://dx.doi.org/10.1007/BF02545207
López-Amorós ML, Hernández T, Estrella I. 2006. Effect of germination on legume phenolic compounds and their antioxidant activity. J. Food Compos. Anal. 19, 277–283. http://dx.doi.org/10.1016/j.jfca.2004.06.012
Mectalfe LD, Schmitz AA, Pellka JR .1966. Rapid preparation of fatty acids esters from lipids for gas-chromatographic analysis. Anal. Chem. 38, 514–515. http://dx.doi.org/10.1021/ac60235a044
Minguez-Mosquera MI, Rejano-Navarro L, Gandulrojas B, Sanchez Gomez AH, Garrido-Fernandez J. 1991. Colorpigment correlation in virgin olive oil. J. Am. Oil Chem. Soc. 86, 332–336. http://dx.doi.org/10.1007/BF02657688
Mondal N, Bhat KV, Srivastava PS. 2010. Variation in fatty acid composition in Indian germplasm of sesame. J. Am. Oil Chem. Soc. 87, 1263–1269. http://dx.doi.org/10.1007/s11746-010-1615-9
Randhir R, Lin Y, Shetty K. 2004. Stimulation of phenolics, antioxidant and antimicrobial activities in dark germinated mung bean sprouts in response to peptide and phytochemical elicitors. Process Biochem. 39, 637–646. http://dx.doi.org/10.1016/S0032-9592(03)00197-3
Sebei K, Debez A, Herchi W, Boukhchina S, Kallel H. 2007. Germination kinetics and seed reserve mobilization in two flax (Linum usitatissimum L.) cultivars under moderate salt stress, J Plant Biol. 50, 447–454. http://dx.doi.org/10.1007/BF03030681
Siger A, Nogala-Kalucka M, Lampart-Szczapa E. 2008. The content and antioxidant activity of phenolic compounds in cold pressed plant oils. J. Food Lipids. 15, 137–149. http://dx.doi.org/10.1111/j.1745-4522.2007.00107.x
Subramanian R, Nandini KE, Sheila PM, Gopalakrishna AG, Raghavarao KSMS, Nakajima M, Kimura T, Maekawa T. 2000. Membrane processing of used frying oils. J. Am. Oil Chem. Soc. 77, 323–328. http://dx.doi.org/10.1007/s11746-000-0052-2
Teh S-S, Birch J. 2013. Physicochemical and quality characteristics of cold-pressed hemp, flax and canola seed oils. J. Food Compos. Anal. 30, 26–31. http://dx.doi.org/10.1016/j.jfca.2013.01.004
Touré A, Xueming X. 2010. Flaxseed Lignans: Source, Biosynthesis, Metabolism, Antioxidant Activity, Bio-Active Components, and Health Benefits. Compr. Rev. Food. Sci. F. 9, 261–269.
Vaisey-Genser M, Morris DH. 1997. Flaxseed: Health, Nutrition and Functionality. Winnipeg, MB: Flax Council of Canada.
Vaisey-Genser M, Morris DH. 2001. History of cultivation and uses of flaxseed. In Flax, The genus Linum. Edited by: Muir A, Westscott N. Amsterdam: Hardwood Academic Publishers, 1–21.
Wanasundara PKJPD, Shahidi F, Brosnan ME. 1999a. Changes in Flax (Linum usitatissmum) seed nitrogenous compounds during germination. Food Chem. 65, 289–295. http://dx.doi.org/10.1016/S0308-8146(98)00176-9
Wanasundara PKJPD, Wanasundara UN, Shahidi F. 1999b. Changes in flax (Linum usitatissimum L.) seed lipids during germination. J. Am. Oil Chem. Soc. 76, 41–48. http://dx.doi.org/10.1007/s11746-999-0045-z
Wong SP, Leong LP, William Koh JH. 2006. Antioxidant activities of aqueous extracts of selected plants. Food Chem. 99, 775–783. http://dx.doi.org/10.1016/j.foodchem.2005.07.058
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