Composition and characterization by GC-MS of the essential oil extracted from Nicotiana glauca Graham




Essential oil, Eugenol, GC-MS, Hydrodistillation, N. glauca


Fresh leaves of N. glauca were collected from the northern region of Tunisia. The leaves were submitted to water distillation for 4 h, using a Clevenger-type apparatus. The obtained essential oils were dried over anhydrous sodium sulphate and after filtration, stored at 4 °C until use. The chemical composition of the isolated essential oil was analyzed by gas chromatography–mass spectrometry (GC–MS). Eighteen compounds were identified: eugenol, the major compound in the essential oil, was present at a high level (58.49%), followed by nonadecane, eugenyl acetate and tridecane, 3-methyl at 6.38; 5.57 and 5.19%, respectively. The percentage of compounds dodecane, 2, 6, 11 trimethyl, tetradecane, docosane, tricosane and 1, 2-benzene dicarboxilic, dibutyl ester varied between 1 and 2%; whereas the other compounds (including limonene, and saturated hydrocarbons) remained at low percentages, not exceeding 1%. This study could be very useful for the characterization, pharmaceutical and therapeutic applications of the essential oil from N. glauca.


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Adams RP. 2007. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry, 4th Edition., Allured Pub Corp, Carol Stream, Ill.

Bao LM et al. 2012. Hydrolysable tannins isolated from Syzygium aromaticum: Structure of a new C-Glucosidic ellagitannin and spectral features of tannins with a tergalloyl group. Heterocycles 85, 365-381.

Bauer K, Garbe D, Surburg H. 2008. Common Fragrance and Flavor Materials: Preparation, Properties and Uses, 3rd Edition. Available at: and+Flavor+Materials%3A+Preparation%2C+ Properties+and+Uses%2C+ 3rd+ Edition-p- 9783527612376 [Accessed July 3, 2018].

Dang MN et al. 2001. Antioxidant activity of essential oils from various spices. Nahr. 45, 64-66.<64::AID-FOOD64>3.0.CO;2-U

Digrak M et al. 1999. Antibacterial and Antifungal Effects of Various Commercial Plant Extracts. Pharm. Biol. 37, 216-220.

Grassmann J et al. 2000. Antioxidant properties of essential oils. Possible explanations for their anti-inflammatory effects. Arzneimittelforschung 50, 135-139.

Kaczorowski RL, Markman S. 2016. Nectar alkaloids of tree tobacco can reduce Palestine sunbird foraging performance in a colour discrimination task. Anim. Behav. 119, 59-68.

Leete E, Chedekel MR. 1973. Metabolism of nicotine in Nicotiana glauca. Phytochemistry 13, 1853-1859.

Manoguerra AS, Freeman D. 1982. Acute Poisoning from the Ingestion of Nicotiana glauca. J. Toxicol. Clin. Toxicol. 19, 861-864. PMid:7182515

Marotti M et al. 1994. Effects of planting time and mineral fertilization on peppermint (Mentha x Piperita L.) essential oil composition and its biological activity. Flavour Fragr. J. 9, 125-129.

Marotti M, Piccaglia R, Giovanelli E. 1996. Differences in Essential Oil Composition of Basil (Ocimum basilicum L.) Italian Cultivars Related to Morphological Characteristics. J. Agric. Food Chem. 44, 3926-3929.

Morel AF et al. 1998. A new amide from Nicotiana glauca. Planta Med. 64, 284-285. PMid:17253244

Nakamura CV et al. 1999. Antibacterial activity of Ocimum gratissimum L. essential oil. Mem. Inst. Oswaldo Cruz 94, 675-678. PMid:10464416

Pandey SN, Chada A. 1998. A Textbook of Botany: Plant Anatomy and Economic Botany Vol 3 10th edition., Sangam Books Ltd, New Delhi.

Panter KE, James LF, Gardner DR. 1999. Lupines, poison-hemlock and Nicotiana spp: toxicity and teratogenicity in livestock. J. Nat. Toxins 8, 117-134.

Popova V et al. 2017. Biologically Active and Volatile Compounds in Leaves and Extracts of Nicotiana alata Link & Otto from Bulgaria. J. Pharm. Sci. Res. 9, 2045-2051.

Price S. 1998. Aromatherapy Workbook: Understanding Essential Oils - From Plant to Bottle, Thorsons, London.

Satil F, Azcan N, Baser KHC. 2003. Fatty Acid Composition of Pistachio Nuts in Turkey. Chem. Nat. Compd. 39, 322-324.

Schlotzhauer WS et al. 1995. Comparison of the Volatile Flower Oils of Nicotiana rustica and N. forgetiana. J. Essent. Oil Res. 7, 265-269.

Sims DN, James R, Christensen T. 1999. Another death due to ingestion of Nicotiana glauca. J. Forensic Sci. 44, 447-449.

Skliar null et al. 2000. Nicotiana glauca: another plant species containing vitamin D(3) metabolites. Plant Sci. Int. J. Exp. Plant Biol. 156, 193-199.

Tabana YM et al. 2016. Scopoletin, an active principle of tree tobacco (Nicotiana glauca) inhibits human tumor vascularization in xenograft models and modulates ERK1, VEGF-A, and FGF-2 in computer model. Microvasc. Res. 107, 17-33. PMid:27133199

Telci I et al. 2006. Variability in essential oil composition of Turkish basils (Ocimum basilicum L.). Biochem. Syst. Ecol. 34, 489-497.

Van Wyk B-E, Gericke N. 2000. People's plants: A guide to useful plants of Southern Africa., Briza Publications.

Watt J, Breyer-Brandwijk M. 1962. The Medicinal and Poisonous Plants of Southern and Eastern Africa - Auction #28 | Available at: [Accessed July 2, 2018].



How to Cite

Cherif A, Ammar S, Boukhchina S. Composition and characterization by GC-MS of the essential oil extracted from Nicotiana glauca Graham. Grasas aceites [Internet]. 2019Sep.30 [cited 2024Apr.14];70(3):e317. Available from:




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