Potentials of enhancing the physicochemical and functional characteristics of Nigella sativa oil by using the screw pressing technique for extraction





Crude oil, Functionality, Hydraulic pressing, Nigella sativa, Screw pressing


In the current investigation the crude oil of Nigella sativa was extracted from seeds using hydraulic and screw pressing techniques. Different parameters were evaluated in order to find out the appropriate technique to enhance the physicochemical and functional-related characteristics of the extracted crude oil. Results showed that the acid and peroxide values were significantly lower in the screw pressed oil (SPO) than in the hydraulic pressed oil (HPO). The total phenolic content of the SPO was significantly higher than that of HPO. Evaluation of the oxidative stability using the Rancimat test showed that SPO recorded a much higher oxidative stability index (40.07 h) than HPO (0.51 h). The yield of the volatile oil fraction and its contents of thymoquinone isolated from the SPO were higher than that from the HPO. Biological evaluation revealed that the SPO had significantly higher antimicrobial activity than HPO against Listeria monocytogenes and Staphylococcus aureus at 40 μL/well.


Download data is not yet available.


Adams, R. P. (2007). Identification of essential oil components by gas chromatography/quadrupole mass spectroscopy (4th ed.). Illinois: Allured Publishing Corporation.

Akinoso R, Raji A, Igbeka J. 2009. Effects of compressive stress, feeding rate and speed of rotation on palm kernel oil yield. J. Food Eng. 93, 427–430. https://doi.org/10.1016/j.jfoodeng.2009.02.010

Al-Okbi S, Mohamed D, Hamed T, Edris A. 2015. Evaluation of the therapeutic effect of Nigella sativa crude oil and its combination with omega-3 fatty acids-rich oils in a modified hepatorenal syndrome model in rats. Grasas Aceites 66, e103. https://doi.org/10.3989/gya.0245151

Al-Okbi S, Mohamed D, Hamed T, Edris A. 2013. Potential protective effect of Nigella sativa crude oils towards fatty liver in rats. Eur. J. Lipid Sci. Tech. 115, 774–782. https://doi.org/10.1002/ejlt.201200256

AOCS Official Methods of Analysis. Methods Cd 8–53; Cd 3d-63; Cd 3–25; Cd 3d-63; AOCS: Champaign, Illinois, USA, 1997.

AOCS Official Methods of Analysis. Method Cc 13b-45; AOCS: Champaign, Illinois, USA, 1998.

Biljana R, Etelka D, Miroslav N, Vele T, Zorica B. 2014. The most important bioactive components of cold pressed oil from different pumpkin (Cucurbita pepo L.) seeds. LWT-Food Sci. Tech. 55, 521–527.

Birk E, Mannheim C, Saguy I. 1998. A rapid method to monitor quality of apple juice during thermal processing. Lebensm. Wiss. Technol. 31, 612–616. https://doi.org/10.1006/fstl.1998.0385

Chaieb K, Kouidhi B, Jrah H, Mahdouani K, Bakhrouf A. 2011. Antibacterial activity of thymoquinone; an active principle of N. sativa and its potency to prevent bacterial biofilm formation. BMC Complemen. Alt. Med. 11, 29–34. https://doi.org/10.1186/1472-6882-11-29 PMid:21489272 PMCid:PMC3095572

Cheikh-Rouhou S, Besbes S, Hentati B, Blecker C, Deroanne C, Attia H. 2007. Nigella sativa L.: Chemical composition and physicochemical characteristics of lipid fraction. Food Chem. 101, 673–681. https://doi.org/10.1016/j.foodchem.2006.02.022

Codex Alimentarius, 1999. Guidelines for the production, processing, labelling and marketing of organically produced foods.

Dandik L, Aksoy H. 1992. The kinetics of hydrolysis of Nigella Sativa (Black Cumin) seed oil catalyzed by native lipase in ground seed. J. Am. Oil Chem. Soc. 69, 1239–1241. https://doi.org/10.1007/BF02637689

Darakhshan S, Pour A, Colagar A, Sisakhtnezhad S. 2015. Thymoquinone and its therapeutic potentials. Pharm. Res. 95–96, 138–158. https://doi.org/10.1016/j.phrs.2015.03.011 PMid:25829334

Develi S, Evran B, Kalaz E, Koçak-Toker N, Erata G. 2014. Protective effect of Nigella sativa oil against binge ethanol- induced oxidative stress and liver injury in rats. Chin. J. Nat. Med. 12, 495–499. https://doi.org/10.1016/S1875-5364(14)60077-7

Edris A. 2011. The chemical composition and the content of volatile oil: potential factors that can contribute to the oxidative stability of Nigella sativa L. crude oil. J. Diet. Suppl. 8, 34–42. https://doi.org/10.3109/19390211.2010.547242 PMid:22432633

Gholamnezhad Z, Havakhah S, Boskabady M. 2016. Preclinical and clinical effects of Nigella sativa and its constituent, thymoquinone: A review. J. Ethnopharmacol. 190, 372–386. https://doi.org/10.1016/j.jep.2016.06.061 PMid:27364039

Gül?en I, Ak H, Çölçimen N, Alp H, Akyol M, Demir I, Atalay T, Balahro?lu R, Ra?betli M. 2016. Neuroprotective effects of thymoquinone on the hippocampus in a rat model of traumatic brain injury. World Neurosurg. 86, 243–249.

Gutfinger T. 1981. Polyphenols in olive oils. J. Am. Oil Chem. Soc. 58, 966–968. https://doi.org/10.1007/BF02659771

Hadjadj N, Acheheb H, Aitchaouche F, Belhachat D, Ferradji A. 2014. Optimization of oil extraction from Nigella sativa seeds by pressing using response surface methodology. Am. J. Food Tech. 9, 136–143. http://dx.doi.org/10.3923/ ajft.2014.136.143

Ismail M, Al-naqeep G, Chan W. 2009. Nigella sativa thymoquinone-rich fraction greatly improves plasma antioxidant capacity and expression of antioxidant genes in hypercholesterolemic rats. Free Rad. Biol. Med. 48, 664–672. https://doi.org/10.1016/j.freeradbiomed.2009.12.002 PMid:20005291

Khoddami A, Ghazali H, Yassoralipour A, Ramakrishnan Y, Ganjloo A. 2011. Physicochemical characteristics of Nigella seed (Nigella sativa L.) oil as affected by different extraction methods. J. Am. Oil Chem. Soc. 88, 533–540. https://doi.org/10.1007/s11746-010-1687-6

Kiralan M, Özkanb G, Bayrakc A, Ramadan F. 2014. Physicochemical properties and stability of black cumin (Nigella sativa) seed oil as affected by different extraction methods. Ind. Crops Prod. 57, 52–58. https://doi.org/10.1016/j.indcrop.2014.03.026

Lutterodt H, Luther M, Slavin M, Yin J, Parry J, Gao M, Yu L. 2010. Fatty acid profile, thymoquinone content, oxidative stability, and antioxidant properties of cold-pressed black cumin seed oils. LWT - Food Sci. Tech. 43, 1409–1413.

Mason J, Fu M, Chen J, Thompson L. 2015. Flaxseed oil enhances the effectiveness of trastuzumab in reducing the growth of HER2-overexpressing human breast tumors (BT-474). J. Nutr. Biochem. 26, 16–23. https://doi.org/10.1016/j.jnutbio.2014.08.001 PMid:25441844

Miyashita K, Takagi T. 1986. Study on the oxidative rate and prooxidant activity of free fatty acids. J. Am. Oil Chem. Soc. 63, 1380–1384. https://doi.org/10.1007/BF02679607

Perez C, Pauli M, Bazerque P. 1990. An antibiotic assay by the well agar diffusion method. Acta Bio. Med. Exp. 15, 113–115.

Rice-Evans C, Miller N, Paganga G. 1997. Antioxidant properties of phenolic compounds. Trend Plant Sci. 2, 152–159. https://doi.org/10.1016/S1360-1385(97)01018-2

Salman M, Khan R, Shukla I. 2008. Antimicrobial activity of Nigella sativa Linn. seed oil against multi-drug resistant bacteria from clinical isolates. Nat. Prod. Rad. 7, 10–14.

Schneider-Stock R, Fakhoury H, Zaki M, El-Baba O, Gali- Muhtasib H. 2014. Thymoquinone: fifty years of success in the battle against cancer models. Drug Discov. Today 19, 18–30. https://doi.org/10.1016/j.drudis.2013.08.021 PMid:24001594

Tulukcu F. 2011. A comparative study on fatty acid composition of black cumin obtained from different regions of Turkey, Iran and Syria. Afric. J. Agric. Res. 6, 892–895.

Ustun G, Kent L, Cekin N, Civelekoglu H. 1990. Investigation of the technological properties of N. sativa (black cumin) seed oil. Am. Oil Chem. Soc. 67, 958–960. https://doi.org/10.1007/BF02541857

Walkelyn J, Wan J. 2006. Solvent extraction to obtain edible oil products, in Akoh C (Ed.), Handbook of Functional Lipids. Boca Raton, CRC Press, pp. 89–131.

Willems P, Kuipers N, De Haan A. 2008. Hydraulic pressing of oilseeds: Experimental determination and modeling of yield and pressing rates. J. Food Eng. 89, 8–16. https://doi.org/10.1016/j.jfoodeng.2008.03.023

Zzaman W, Silvia D, Abdullah W, Yang A. 2014. Physicochemical and quality characteristics of cold and hot press of Nigella sativa L seed oil using screw press. J. Appl. Sci. Res. 10, 36–45.



How to Cite

Hamed SF, Shaaban HA, Ramadan AA, Edris AE. Potentials of enhancing the physicochemical and functional characteristics of Nigella sativa oil by using the screw pressing technique for extraction. Grasas aceites [Internet]. 2017Jun.30 [cited 2024Mar.4];68(2):e188. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1655