Evaluación del efecto terapéutico del aceite crudo de Nigella sativa y su mezcla con aceites ricos en ácidos grasos omega-3 en un modelo de síndrome hepatorenal modificado en ratas
DOI:
https://doi.org/10.3989/gya.0245151Palabras clave:
Aceite crudo Nigella, Ácidos grasos omega-3, Mezcla de aceites, Síndrome hepatorenalResumen
En el presente estudio, el efecto hepato- y reno-protector de aceites crudos de Nigella sativa y su mezcla binaria con aceites ricos en ácidos grasos omega-3 (pescado y aceites de linaza) fue estudiado en un modelo modificado de síndrome hepatorenal (MHRS) en ratas. MHRS fue inducido a través de la alimentación de una dieta alta en fructosa seguido de la inyección intraperitoneal de clorhidrato de galactosamina. Diferentes aceites fueron suministrados como dosis oral diaria a ratas con MHRS. Se realizaron dos grupos de control de MHRS y ratas sanas normales. Se evaluaron diferentes parámetros bioquímicos y nutricionales. La inducción de la MHRS produce disfunción hepática y renal, elevado estrés oxidativo y biomarcadores inflamatorios, endotelina 1, y colesterol en plasma. Se observó una reducción del colesterol plasmatico de lipoproteínas de alta densidad, albúmina y Ca, elevación en orina de N-acetil-β-D-glucosaminidasa y de la grasa del hígado. La administración de aceite crudo de Nigella que tiene un 0,2% de ácidos grasos omega-3 totales o su mezcla con aceite de pescado (17,9% de omega-3) o aceite de linaza (42,1% de omega-3) mejoró significativamente todos los parámetros bioquímicos de MHRS. No hubo diferencia significativa en los parámetros bioquímicos entre los diferentes grupos tratados con los aceites ensayados independientemente del contenido de ácidos grasos omega-3. Con esto se puede resaltar el potencial efecto de la fracción volátil del aceite crudo de Nigella.
Descargas
Citas
Abdel-Moneima E, Dkhila A, Al-Quraishy S. 2011. The protective effect of flaxseed oil on lead acetate-induced renal toxicity in rats. J. Hazard. Mater. 194, 250–255. http://dx.doi.org/10.1016/j.jhazmat.2011.07.097 PMid:21872391
Al-Bishri M. 2013. Favorable effects of flaxseed supplemented diet on liver and kidney functions in hypertensive Wistar rats. J. Oleo Sci. 62, 709–715. http://dx.doi.org/10.5650/jos.62.709 PMid:24005015
Al-Okbi S, Ammar N, Abd El-Kader M. 1997. Studies of some biochemical, nutritional and anti-inflammatory effects of Nigella Sativa seeds. Egypt. J. Pharmaceut. Sci. 38, 451–469.
Al-Okbi S, Mohamed D. 2012. Preparation and Evaluation of functional foods in adjuvant arthritis. Grasas Aceites. 63, 394-402. http://dx.doi.org/10.3989/gya.130811
Al-Okbi S, Mohamed D, Hamed Th, Edris A. 2013. Potential protective effect of Nigella sativa crude oils towards fatty liver in rats. Eur. J. Lipid Sci. Technol. 115, 774–782. http://dx.doi.org/10.1002/ejlt.201200256
Al-Okbi S, Mohamed D, Hamed Th, Esmail S, Donya M. 2014. Prevention of renal dysfunction by nutraceuticals prepared from oil rich plant foods. Asian Pacific J. Trop. Biomed. 4, 618–627. http://dx.doi.org/10.12980/APJTB.4.201414B66 PMid:25183331 PMCid:PMC4037655
Al-Suhaimi A. 2012. Hepatoprotective and immunological functions of Nigella sativa seed oil against hypervitaminosis A in adult male rats. Inter. J. Vitamin and Nutr. Res. 82, 288–297. http://dx.doi.org/10.1024/0300-9831/a000121 PMid:23591666
Anand R, Harry D, Holt S, Milner P, Dashwood M, Goodier D, Jarmulowicz M, Moore K. 2002. Endothelin is an important determinant of renal function in a rat model of acute liver and renal failure. Gut. 50, 111–117. http://dx.doi.org/10.1136/gut.50.1.111 PMid:11772977 PMCid:PMC1773076
Ansari I, Sheikh A, Ahmed S, Jabbar Q, Ali S. 2014. Management of anemia and other hematologic derangements in patients with chronic kidney disease. Arab J. Nephrol. Transplant. 7, 13–19. PMid:24702529
Arroyo V, Ginès P, Gerbes A, Dudley J, Gentilini P, Laffi G, Reynolds B, Ring-Larsen H, Schölmerich J. 1996. Definition and diagnostic criteria of refractory ascites and hepatorenal syndrome in cirrhosis. Hepatol. 23, 164–176. http://dx.doi.org/10.1002/hep.510230122 PMid:8550036
Aydogdu S, Akil I, Akil T, Akil T, Kabasakal C, Killi R, Mir S, Yag˘ci R. 2004. Renal resistive indexes and some renal functions in liver cirrhotic children. Pediatr. Int. 46, 67–71. http://dx.doi.org/10.1111/j.1442-200X.2004.01826.x PMid:15043668
Azar R, Dequiedt F, Awada J, Dequiedt P, Tacquet A. 1989. Effects of fish oil rich in polyunsaturated fatty acids on hyperlipidemia of hemodialysis patients. Kidney Int. 27, S239-S242.
Baggio B, Budakovic A, Priante G, Gambaro G, Manzato E, Khan S. 2002. Dietary fatty acid supplementation modulates the urinary excretion of calcium and oxalate in the rat. Insight into calcium lithogenesis. Nephron. 91, 486–491. http://dx.doi.org/10.1159/000064292 PMid:12119482
Bataller R, Sort P, Gine's P, Arroyo V. 1998. Hepatorenal syndrome: definition, pathophysiology, clinical features and management. Kidney Int. Suppl. 66, S47–S53. PMid:9573573
Bibus M, Stitt P, Holman T. 1998. Ratios of linoleic to a-linolenic acids in the diet: Implications in the health of humans and companion animals, in Proceedings of the 57th Flax Institute of the U.S., Fargo, ND, pp. 49–52.
Briggs M, Williams A. 1963. A new mineral mixture for experimental rat diets and evaluation of other mineral mixtures. Feder. Proceedings. 22, 261–266.
Burstein M, Scholnick H, Morfin R. 1970. Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions. J. Lipid Res. 11, 583–595. PMid:4100998
Caligiuri P, Love K, Winter T, Gauthier J, Taylor G, Blydt-Hansen T, Zahradka P, Aukema M. 2013. Dietary linoleic acid and α-linolenic acid differentially affect renal oxylipins and phospholipid fatty acids in diet-induced obese rats. J. Nutrit. 143, 1421–1431. http://dx.doi.org/10.3945/jn.113.177360 PMid:23902961
Cequier-Sánchez E, Rodríguez C, Ravelo G, Zárate R. 2008. Dichloromethane as a solvent for lipid extraction and assessment of lipid classes and fatty acids from samples of different natures. J. Agric. Food Chem. 56, 4297–4303. http://dx.doi.org/10.1021/jf073471e PMid:18505264
Cheikh-Rouhou S, Besbes S, Lognay G, Blecker C, Deroanne C, Attia H. 2008. Sterol composition of black cumin (Nigellas ativa L.) and Aleppo pine (Pinus halepensis Mill.) seed oils. J. Food Comp. Anal. 21, 162–168. http://dx.doi.org/10.1016/j.jfca.2007.09.001
Clark F, Parbtani A, Huff W, Spanner E, de Salis H, Chin-Yee I, Philbrick J, Holub J. 1995. Flaxseed: a potential treatment for lupus nephritis. Kidney Int. 48, 475–483. http://dx.doi.org/10.1038/ki.1995.316 PMid:7564115
Davenport A, Ahmad J, Khafaji A, Kellum J, Genyk S, Nadim K. 2012. Medical management of hepatorenal syndrome. Nephrol. Dial Transpl. 27, 34–41. http://dx.doi.org/10.1093/ndt/gfr736 PMid:22287700
De Caterina R, Endres S, Kristesen S, Schmidt E. 1994. n-3 Fatty acids and renal diseases. Amer. J. Kidney Dis. 24, 397–415. http://dx.doi.org/10.1016/S0272-6386(12)80896-1
Demirbas T, Piskin T, Dayangac M, Yaprak O, Oklu L, Yuzer Y, Tokat Y. 2012. Successful treatment of severe hepatorenal syndrome with living donor liver transplantation. Hepatogastroenterol. 59, 2305–2306.
Donadio V. 2001. n-3 Fatty acids and their role in nephrologic practice. Current Opin. Nephrol. Hyperten. 10, 639–642. http://dx.doi.org/10.1097/00041552-200109000-00014 PMid:11496058
Doumas T, Watson A, Biggs G. 1972. Albumin standards and the measurement of serum albumin with bromocresol green. Clin. Chem. Acta. 31, 87–96. http://dx.doi.org/10.1016/0009-8981(71)90365-2
European Pharmacopoeia 1996. Conseil de l'Europe: Pharmacopée Européenne. France: Maisonneuve S.A., Sainte Ruffine.
Fagundes C, Ginè P. 2012. Hepatorenal syndrome: A severe, but treatable, cause of kidney failure in cirrhosis. Am. J. Kidney Dis. 59, 874–885. http://dx.doi.org/10.1053/j.ajkd.2011.12.032 PMid:22480795
Fawcett K, Scott E. 1960. A rapid and precise method for the determination of urea. J. Clin. Pathol. 13, 156–159. http://dx.doi.org/10.1136/jcp.13.2.156 PMid:13821779 PMCid:PMC480024
Fernandez R, Piechnik J, Fabris R, Malnic G, Fernandes L. 2004. Effect of chronic fish oil supplementation on renal function of normal and cachectic rats. Brazil. J. Med. Biolog. Res. 37, 1481–1489. http://dx.doi.org/10.1590/S0100-879X2004001000006
Folch J, Lees M, Sloane-Stanley H. 1957. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226, 497–509. PMid:13428781
Gambino R. 1965. Standard Methods of Clinical Chemistry. Academic Press, New York.
Garcia-Martinez R, Caraceni P, Bernardi M, Gines P, Arroyo V, Jalan R. 2013. Albumin: Pathophysiologic basis of its role in the treatment of cirrhosis and its complications. Hepatol. 58, 1836–1846. http://dx.doi.org/10.1002/hep.26338 PMid:23423799
Harats D, Dabach Y, Hollander G, Ben-Naim M, Schwartz R, Berry EM, Stein O, Stein Y. 1991. Fish oil ingestion in smokers and nonsmokers enhances peroxidation of plasma lipoproteins. Atheroscler. 90, 127–139. http://dx.doi.org/10.1016/0021-9150(91)90107-E
Higdon V, Liu J, Du H, Morrow D, Ames N, Wander C. 2000. Supplementation of postmenopausal women with fish oil rich in eicosapentaenoic acid and docosahexaenoic acid is not associated with greater in vivo lipid peroxidation compared with oils rich in oleate and linoleate as assessed by plasma malondialdehyde and F2-isoprostanes1–3. Am. J. Clin. Nutr. 72, 714–722. PMid:10966889
Houcher Z, Boudiaf K, Benboubetra M, Houcher B. 2007. Effects of methanolic extract and commercial oil of Nigella sativa L. on blood glucose and antioxidant capacity in alloxan-induced diabetic Rats. Pteridines. 18, 8–18. http://dx.doi.org/10.1515/pteridines.2007.18.1.8
Houot O. 1985. Interpretation of Clinical Laboratory Tests. Biomedical Publications. (Doi not available).
Jenkins G, Kendall W, Vidgen E, Agarwal S, Rao V, Rosenberg S, Diamandis P, Novokmet R, Mehling C, Perera T, Griffin C, Cunnane C. 1999. Health aspects of partially defatted flaxseed, including effects on serum lipids, oxidative measure and ex vivo androgen and progestin activity: a controlled cross-ovar trial. Am. J. Clin. Nutr. 69, 395–403. PMid:10075322
Kamisako T, Miyawaki S, Gabazza C, Ishihara T, Kamei A, Kawamura N, Adachi Y. 1998. Polyethylene glycol-modified bilirubin oxidase improves hepatic energy charge and urinary prostaglandin levels in rats with obstructive jaundice. J. Hepatol. 29, 424–429. http://dx.doi.org/10.1016/S0168-8278(98)80060-3
Kawasaki T, Igarashi K, Koeda T, Sugimoto K, Nakagawa K, Hayashi S, Yamaji R, Inui H, Fukusato T, Yamanouchi T. 2009. Rats fed fructose-enriched diets have characteristics of nonalcoholic hepatic Steatosis. J. Nutr. 139, 2067–2071. http://dx.doi.org/10.3945/jn.109.105858 PMid:19776184
Koracevic D, Koracevic G, Djordjevic V, Anderejevic S, Cosic V. 2001. Methods for the measurement of antioxidant activity in human fluids. J. Clin. Pathol. 54, 356–361. http://dx.doi.org/10.1136/jcp.54.5.356 PMid:11328833 PMCid:PMC1731414
Mattson H, Grundy M. 1985. Comparison of effects of dietary saturated, monounsaturated, and polyunsaturated fatty acids on plasma lipids and lipoproteins in man. J. Lipid Res. 26, 194–202. PMid:3989378
Megraw R, Dunn D, Biggs H. 1979. Manual and continuous flow colorimetry of triglycerols by a fully enzymatic method. Clin. Chem. 25, 273–284. PMid:759021
Mensink P, Katan B. 1992. Effect of dietary fatty acids on serum lipids and lipoproteins. A meta-analysis of 27 trials. Arterioscler. Thrombo. Vascular Biol. 12, 911–919. http://dx.doi.org/10.1161/01.ATV.12.8.911
Meydani M, Natiello F, Goldin B, Free N, Woods M, Schaefer E, Blumberg B, Gorbach L. 1991. Effect of long-term fish oil supplementation on vitamin E status and lipid peroxidation in women. J. Nutr. 121, 484–91. PMid:1826131
Mohamed A, El-Hariri M, Al-Okbi Y. 2005. Impact of feeding bread enriched with flaxseed on plasma profile of hyperlipidemic rats. Polish J. Food Nutr. Sci. 14 (4), 431–436.
Mohindra S, Kumar K. 2013. Hepatorenal syndrome clinical queries. Nephrol. 2, 205–211.
Morcos R. 1967. The effect of protein value of the diet on the neurological manifestations produced in rats by b–immodipropionitrile. British J. Nutr. 21, 269–274. http://dx.doi.org/10.1079/BJN19670029 PMid:4952258
Mu-oz S. 2008. The hepatorenal syndrome. Medical Clin. 92 (4), 813–837.
Oomah D, Mazza G. 1995. Flaxseed dietary fiber and cyanogens, in Cunnane, S., Thompson, L (Ed.) Flaxseed in Human Nnutrition. Champaign, AOCS Press Illinois, USA, pp. 56–67.
Planas R, Montoliu S, Balleste B, Rivera M. Miquel M, Masnou H, Galeras A, Giménez D, Santos J, Cirera I, Morillas M, Coll S, Solà R. 2006. Natural history of patients hospitalized for management of cirrhotic ascites. Clin. Gastroenterol. Hepatol. 4, 1385–1394. http://dx.doi.org/10.1016/j.cgh.2006.08.007 PMid:17081806
Price G, Whiting H. 1992. Urinary enzymes and nephrotoxicity in humans, in Jung K, Mattenheimer H, Burchardt U (Ed.) Urinary Enzymes Springer-Verlag, Berlin, pp. 203–221. http://dx.doi.org/10.1007/978-3-642-84313-6_14 PMid:1563256
Pszczola E. 1998. The ABCs of nutritional ingredients. Food Technol. 52, 30–38.
Ramadan F, Wahdan K. 2012. Blending of corn oil with black cumin (Nigella sativa) and coriander (Coriandrum sativum) seed oils: Impact on functionality, stability and radical scavenging activity. Food Chem. 132, 873–879. http://dx.doi.org/10.1016/j.foodchem.2011.11.054
Raptis D, Limani P, Jang J, Ungethu.m U, Tschuor C, Graf R, Humar B, Clavien A. 2014. GPR120 on Kupffer cells mediates hepatoprotective effects of ω3-fatty acids. J. Hepatol. 60, 625–632. http://dx.doi.org/10.1016/j.jhep.2013.11.006 PMid:24262133
Reitman S, Frankel S. 1957. Colorimetric methods for aspartate and alanine aminotransferase. Am. J. Clin. Pathol. 28, 55–60.
Saracyn M, Patera J, Kocik J, Brytan M, Zdanowski R, Lubas A, Kozłowski W, Wan’kowicz Z. 2012. Strain of experimental animals and modulation of nitric oxide pathway: their influence on development of renal failure in an experimental model of hepatorenal syndrome. Arch. Medical Sci. 8, 555–562. http://dx.doi.org/10.5114/aoms.2012.29281 PMid:22852015 PMCid:PMC3400905
Saracyn M, Wesołowski P, Nowak Z, Patera J, Kozłowski W, Wan’kowicz Z. 2008. Race of experimental animals and development of renal failure in animal model of hepatorenal syndrome. Polish Merkur Lekarski. 24, 298–302.
Satoh K. 1978. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clinica Chimica Acta. 20, 37–43.
Schriewer H, Kohnert U, Assmann G. 1984. Determination of LDL cholesterol and LDL apolipoprotein B following precipitation of VLDL in blood serum with phosphotungstic acid/MgCl2. J. Clin. Chem. Clin. Biochem. 22, 35–40. http://dx.doi.org/10.1515/cclm.1984.22.1.35
Shah N, Mohamed E, Jover-Cobos M, Macnaughtan J, Davies N, Moreau R, Paradis V, Moore K, Mookerjee R, Jalan R. 2013. Increased renal expression and urinary excretion of TLR4 in acute kidney injury associated with cirrhosis. Liver Internation. 33, 398–409. http://dx.doi.org/10.1111/liv.12047 PMid:23402610
Simopoulos P. 1999. Essential fatty acids in health and chronic disease. Am. J. Clin. Nutr. 70, 560S–568S. PMid:10479232
Skálová S. 2005. The diagnostic role of urinary N-acetyl-beta-D-glucosaminidase (NAG) activity in the detection of renal tubular impairment. Acta Medica (Hradec Kralove). 48, 75–80.
Snowdon V, Pellicoro A, Ramachandran P, Mungall W, Jansen M, Lennen R, Aucott R, Kendall T, Hughes J, Iredale J, Fallowfield J. 2013. Relaxin is a renal vasodilator in experimental models of cirrhosis and a potential novel therapy for hepatorenal syndrome in man. The Lancet. 381, S102 (special issue). http://dx.doi.org/10.1016/S0140-6736(13)60542-5
Stepaniak A, Gould E, Sun D, Swanborg H. 1995. A comparative study of experimental autoimmune encephalomyelitis in Lewis and DA rats. J. Immunol. 155, 2762–2769. PMid:7544385
Üstun G, Kent L, Cekin N, Civelekoglu, H. 1990 Investigation of the technological properties of Nigella sativa (black cumin) seed oil. J. Am. Oil Chem. Soc. 67 (12), 958–960. http://dx.doi.org/10.1007/BF02541857
Van Lente F, Sult P. 1989. Assessment of renal function by serum creatinine and creatinine clearance: Glomerular filteration rate estimated by four procedures. Clin. Chem. 35 (12), 2326–2330. PMid:2591052
VanKampen J, Zijlstra G. 1965. Determination of hemoglobin and its derivatives. Advces Clin. Chem. 8, 141–187.
Wadei H, Mai M, Ahsan N, Gonwa T. 2006. Hepatorenal syndrome: pathophysiology and management. Clin. J. Am. Soc. Nephrol. 1, 1066–1079. http://dx.doi.org/10.2215/CJN.01340406 PMid:17699328
Wander C, Du H, Ketchum O, Rowe E. 1996. Tocopherol influences in vivo indices of lipid peroxidation in postmenopausal women given fish oil. J. Nutr. 126, 643–652. PMid:8598549
Watson D. 1960. A simple method for the determination of serum cholesterol. Clin. Chem. Acta. 5, 637–642. http://dx.doi.org/10.1016/0009-8981(60)90004-8
Zhou Z, Davar G, Strichartz G. 2002. Endothelin-1 (ET-1) selectively enhances the activation gating of slowly inactivating tetrodotoxin-resistant sodium currents in rat sensory neurons: A mechanism for the pain-inducing actions of ET-1. J. Neurosci. 22 (15), 6325–6330. PMid:12151509
Publicado
Cómo citar
Número
Sección
Licencia
Derechos de autor 2015 Consejo Superior de Investigaciones Científicas (CSIC)
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
© CSIC. Los originales publicados en las ediciones impresa y electrónica de esta Revista son propiedad del Consejo Superior de Investigaciones Científicas, siendo necesario citar la procedencia en cualquier reproducción parcial o total.
Salvo indicación contraria, todos los contenidos de la edición electrónica se distribuyen bajo una licencia de uso y distribución “Creative Commons Reconocimiento 4.0 Internacional ” (CC BY 4.0). Consulte la versión informativa y el texto legal de la licencia. Esta circunstancia ha de hacerse constar expresamente de esta forma cuando sea necesario.
No se autoriza el depósito en repositorios, páginas web personales o similares de cualquier otra versión distinta a la publicada por el editor.
