Phospholipase A<sub>2</sub> enzyme immobilization

B. Maroto; C. Camusso

doi:10.3989/gya.2000.v51.i3.471

Phospholipase A₂ enzyme immobilization

Authors

B. Maroto Facultad de Ciencias Agropecuarias. Universidad de Córdoba. Rep. Argentina
C. Camusso Facultad de Ciencias Agropecuarias. Universidad de Córdoba. Rep. Argentina

DOI:

https://doi.org/10.3989/gya.2000.v51.i3.471

Keywords:

Immobilization, Phospholipase A2, Support

Abstract

A series of several supports were tested for phospholipase A₂ enzyme (Lecitase 100 S- NOVO) immobilization. This system would find a number of industrial applications such as the selective hydrolysis of soybean lecithin to obtain lysophospholipids (modified lecithins) of great importance in pharmaceuticals and bread making. Successful results have been obtained from the immobilization of Phospholipase A₂ on Alumina, Silica gel, Amberlite IRA-400S, Dowex and Sephadex A-25 via adsorption (the results were in the range 15-30 % of enzyme adsorption). The immobilization reaction was carried out at ambient temperature, with slow magnetic agitation such that the enzyme and 50 mg support were present in the total reaction volume of 5 ml in Tris-ClH buffer (pH 8.65). Several enzyme/supports ratio and the adsorption percentage with respect to pH and temperature and time were investigated. We have compared the behaviour of the phospholipase A₂ in soluble form and immobilized form toward pure powder soybean lecithin as substrate.

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Published

2000-06-30

How to Cite

Maroto B, Camusso C. Phospholipase A2 enzyme immobilization. Grasas aceites [Internet]. 2000Jun.30 [cited 2024Apr.20];51(3):150-6. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/471

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Issue

Vol. 51 No. 3 (2000)

Section

Research

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