Preparation of human milk fat substitute and improvement of its oxidative stability

H.A.  Liu; J.Y.  Huang; T.M.  Olajide; T.  Liu; Z.M.  Liu; X.Y.  Liao; X.C.  Weng

doi:10.3989/gya.0444211

Autores/as

H.A. Liu State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute - School of Life Sciences, Shanghai University https://orcid.org/0000-0002-0528-0860
J.Y. Huang School of Life Sciences, Shanghai University https://orcid.org/0000-0001-7410-0504
T.M. Olajide School of Environmental and Chemical Engineering, Shanghai University https://orcid.org/0000-0003-4751-8715
T. Liu School of Environmental and Chemical Engineering, Shanghai University https://orcid.org/0000-0002-8975-4989
Z.M. Liu State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute https://orcid.org/0000-0003-3777-087X
X.Y. Liao School of Life Sciences, Shanghai University https://orcid.org/0000-0003-1394-1160
X.C. Weng School of Life Sciences, Shanghai University - School of Environmental and Chemical Engineering, Shanghai Universit https://orcid.org/0000-0003-2047-1654

DOI:

https://doi.org/10.3989/gya.0444211

Palabras clave:

1,3-dioleoil-2-palmitoilglicerol, Estabilidad oxidativa, Interesterificación enzimática, Mezcla física, Sucedáneos de grasa de leche humana

Resumen

Se sintetizó el 1,3-dioleoil-2-palmitoilglicerol (OPO), utilizando estearina de palma rica en tripalmitina (PPP) y oleato de etilo, mediante interesterificación enzimática. Se optimizaron los parámetros de la interesterificación enzimática, como la temperatura, el contenido de agua, la carga de enzimas y la relación molar del sustrato. Se lograron altos rendimientos de C52 (principalmente OPO y sus isómeros, 46,7%) y un contenido de ácido palmítico (PA) en sn-2 del 75,3%. Además, el sustituto graso de leche humana OPO (HMFS), se mezcló con aceites de coco, soja, algas y microbianos, en una proporción en peso de 0,70:0,18:0,11:0,004:0,007 para simular los ácidos grasos de la leche humana (HMF) de acuerdo con un modelo matemático. Los ácidos grasos principales e importantes en HMFS-Final estaban casi dentro de los rangos de los presentes en HMF. El HMFS-Final podría promover la absorción de grasas y minerales y el desarrollo de los tejidos de la retina en los bebés. La mezcla de palmitato de L-ascorbilo (L-AP) y vitamina E (VE) resultó tener un efecto antioxidante sinérgico, tanto en la emulsión OPO-HMFS como en la OPO-HMFS. Este hallazgo tiene una gran importancia para mejorar la calidad y prolongar la vida útil de HMFS.

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