Antioxidant activity of 100% and 80% methanol extracts from barley seeds (<i>Hordeum vulgare</i> L.): stabilization of sunflower oil

Farooq Anwar; Hafiz Muhammad Abdul Qayyum; Abdullah Ijaz Hussain; Shahid Iqbal

doi:10.3989/gya.087409

Autores/as

Farooq Anwar Department of Chemistry and Biochemistry, University of Agriculture
Hafiz Muhammad Abdul Qayyum Department of Chemistry and Biochemistry, University of Agriculture
Abdullah Ijaz Hussain Department of Chemistry and Biochemistry, University of Agriculture - Department of Chemistry, Government College University
Shahid Iqbal Department of Chemistry, University of Sargodha

DOI:

https://doi.org/10.3989/gya.087409

Palabras clave:

Aceite de girasol, Almacenamiento acelerado, Antioxidantes, Atrapadores de DPPH, Estabilización, Fenoles, Hordeum vulgare L., Productos de oxidación

Resumen

El potencial antioxidante de extractos de metanol al 100% y el 80% de semillas de tres variedades de cebada (Jou 83, Jou 87 y Haider 93) fue evaluada. El rendimiento de los extractos de las semillas de cebada vario desde un 3.23% (Haider, 100% methanol) a un 5.31% (Jou 83, 80% metanol). El contenido total de fenoles, la actividad atrapadora del radical DPPH (valores IC₅₀) y la inhibición de la oxidación del ácido linoleico de los extractos de semilla de cebada (BSE) fueron 88.1-145.7 mg/100g, 90.8-168.6 μg/mL y 62.6- 74.6%, respectivamente. La efectividad antioxidante de BSE fue también evaluada mediante su capacidad para estabilizar aceite de girasol con concentraciones de BSE de 600 ppm (respecto al peso del aceite). La muestras estabilizadas (tratadas con extractos) y el control (sin adición de extractos) SFO fueron tratadas bajo condiciones de almacenamiento acelerado (calentamiento en un horno a 60ºC durante 30 días y ciclos de calentamiento de 8 h/día). Estas fueron analizadas a intervalos regulares para evaluar la extensión de los cambios oxidativos mediante la medida del valor de peróxidos, valor de para-anisidina y los contenidos de dienos conjugados y trienos congujados. Generalmente, los extractos de semilla de cebada al 80% demostraron una mejor acción antioxidante que los extracto al 100% de metanol. La actividad antioxidante de BSE varió también considerablemente entre las distintas variedades ensayadas. Los presentes resultados sugieren que los extractos antioxidantes de semillas de cebada podrían ser usadas para proteger aceites vegetales de la oxidación.

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