Grasas y Aceites, Vol 50, No 2 (1999)

Preparation of sn-2 long-chain polyunsaturated monoacylglycerols from fish oil by hydrolysis with a stereospecific lipase from mucor miehei


https://doi.org/10.3989/gya.1999.v50.i2.644

Susana Nieto
Unidad de Bioquímica Farmacológica y Lípidos, INTA, Universidad de Chile, Chile

Jorge Gutiérrez
Unidad de Bioquímica Farmacológica y Lípidos, INTA, Universidad de Chile, Chile

Julio Sanhueza
Unidad de Bioquímica Farmacológica y Lípidos, INTA, Universidad de Chile, Chile

Alfonso Valenzuela
Unidad de Bioquímica Farmacológica y Lípidos, INTA, Universidad de Chile, Chile

Abstract


The preparation of sn-2 eicosapentaenoyi glycerol and sn-2 docosahexaenoyi glycerol by the hydrolysis of fish oil by the sn-1, sn-3 stereo-specific immobilised lipase (Lipozyme IM-20) from mucor miehei is described. Monoacylglycerols obtained after the enzymatic hydrolysis were separated by silver nitrate-coated silicic acid column chromatography Both monoacylglycerols can be individually separated in almost pure form by elution from the column with a solvent mixture. The preparation of sn-2 substituted monoacylglycerols from marine origin allows their utilization as substrates for the synthesis of structured long-chain polyunsaturated fatty acid-containing triacylglycerols at specific positions.

Keywords


Enzymatic hydrolysis; Fish oil; Mucor miehei; sn-2 monoacylglycerols (preparation).

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