Role of <i>Bifidobacterium bifidum</i> and plant food extracts in improving microflora and biochemical and cytogenetic parameters in adjuvant arthritis.

S. Y. Al-Okbi; D. A. Mohamed; S. M. Donya; A. B. Abd El Khalek

doi:10.3989/gya.089810

Authors

S. Y. Al-Okbi Food Sciences and Nutrition Department, National Research Centre
D. A. Mohamed Food Sciences and Nutrition Department, National Research Centre
S. M. Donya Cytogenetic Department, National Research Centre
A. B. Abd El Khalek Dairy Department, National Research Centre

DOI:

https://doi.org/10.3989/gya.089810

Keywords:

Adjuvant arthritis, Bifidobacterium bifidum, Biochemical change, Cytogenetic parameters, Microflora, Plant extracts

Abstract

The aim of the present research was to discover plant food extracts and probiotics that may have bioactivity towards chronic inflammation. Three plant food extract mixtures expected to be rich in phenolic compounds, carotenoids and tocopherols were prepared. The anti-inflammatory activity of the different mixtures as well as probiotic bacteria (Bifidobacterium bifidum) were evaluated in adjuvant arthritis in rats. The anti-inflammatory effect, mechanism of action and safety of the three mixtures and Bifidobacterium bifidum were studied by measuring the size of inflammation and the determination of inflammatory and oxidative stress biomarkers, colonic bacteria profile and specific cytogenetic parameters. The contents of tocopherols, β-carotene and phenolic compounds in the mixtures were determined. The results show that the tested mixtures and Bifidobacterium bifidum possess promising anti-inflammatory effects. The mechanism of action seems to involve a reduction in oxidative stress and inflammatory biomarkers and an effect on colonic microflora. Genotoxicity and DNA fragmentation induced by adjuvant arthritis were prevented after supplementation with the tested mixtures.

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References

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