Anti-inflammatory activity of two varieties of pumpkin seed oil in an adjuvant arthritis model in rats
Keywords:Adjuvant arthritis, Anti-inflammatory, β-Carotene, Phenolic contents, Pumpkin seed oils
The aim of the present research was to evaluate the anti-inflammatory activity of pumpkin seed oils (PSOs) of an Egyptian and European variety, in a rat model of adjuvant arthritis. Edema thickness, plasma tumor necrosis factor-α (TNF-α) and erythrocyte sedimentation rate (ESR) were determined as inflammatory biomarkers while malondialdehyde (MDA) and total antioxidant capacity (TAC) were assessed as indicative of oxidative stress. Chromosomal aberration, sperm shape abnormalities, and DNA fragmentations are cytogenetic parameters which aid in tracing inflammatory and oxidative activity. Phenolic contents and β-carotene were determined in PSOs. The results showed elevated ESR, plasma TNF-α, plasma MDA, liver cellular DNA fragmentation, bone marrow chromosomal aberration, sperm shape abnormalities with a reduction in plasma TAC and body weight gain in an adjuvant arthritis control compared to a healthy control. Administration of low and high doses of either Egyptian or European PSO improved all the aforementioned parameters with variable degrees.
Al-Okbi SY, Ammar NM, Soroor KhA, Mohammed DA. 2000a. Impact of natural oils supplements on disease activity and antioxidant state of Egyptian patients with rheumatiod arthritis. Med. J. Islamic Acad. Sci. 13, 161–171.
Al-Okbi SY, Sorour Kh, Abdel-Samed AM, Rashed MM, Mohamed DA. 2000b. Nutritional status and blood anti-oxidant levels of Egyptian rheumatoid arthritis patients. Kasr El Aini Med. J. 6, 43–64.
Al-Okbi SY, Mohamed DA, Donya SM, Abd El Khalek AB. 2011. Role of Bifidobacterium bifidum and Plant Food Extracts in Improving Microflora and Biochemical and Cytogenetic Parameters in Adjuvant Arthritis. Grasas Aceites 62, 308–320. https://doi.org/10.3989/gya.089810
Al-Okbi SY, Mohamed DA. 2012. Preparation and evaluation of functional foods in adjuvant arthritis. Grasas Aceites 63, 394–402. https://doi.org/10.3989/gya.130811
Al-Okbi SY, Mohamed DA, Kandil E, Ahmed EK, Mohammed SE. 2014. Functional ingredients and cardiovascular protective effect of pumpkin seed oils. Grasas Aceites 65, e007. https://doi.org/10.3989/gya.062813
Anderson J, Caplan L, Yazdany J, Robbins ML, Neogi T, Michaud K, Saag KG, O'Dell JR, Kazi S. 2012. Rheumatoid arthritis disease activity measures: American College of Rheumatology recommendations for use in clinical practice. Arthritis Care Res. 64, 640–647. https://doi.org/10.1002/acr.21649 PMid:22473918 PMCid:PMC4028066
Arranz S, Cert R, Pérez-Jiménez J, Cert A, Saura-Calixto F. 2008. Comparison between free radical scavenging capacity and oxidative stability of nut oils. Food Chem. 15, 985–990. https://doi.org/10.1016/j.foodchem.2008.03.021 PMid:26047290
Banji OJ, Banji D, Soumya N, Chilipi KK, Kalpana CH, Kranthi Kumar CH, Annamalai AR. 2014. Combination of carvacrol with methotrexate suppresses Complete Freund's Adjuvant induced synovial inflammation with reduced hepatotoxicity in rats. European J. Pharmacol. 15, 91–98. https://doi.org/10.1016/j.ejphar.2013.12.009 PMid:24333217
Batista AG, Ferrari AS, da Cunha DC, da Silva JK, Cazarin CB, Correa LC, Prado MA, Carvalho-Silva LB, Esteves EA, Marostica Junior MR. 2016. Polyphenols, antioxidants, and antimutagenic effects of Copaifera langsdorffii fruit. Food Chem. 15, 1153–1159. https://doi.org/10.1016/j.foodchem.2015.11.093 PMid:26675852
Glew RH, Glew RS, Chuang LT, Huang YS, Millson M, Constans D, Vanderjagt DJ. 2006. Amino acid, mineral and fatty acid content of pumpkin seeds (Cucurbita spp) and Cyperus esculentus nuts in the Republic of Niger. Plant Foods Human Nutrit. 61, 51–56. https://doi.org/10.1007/s11130-006-0010-z PMid:16770692
Goldring SR. 2000. The final pathogenetic steps in focal bone erosions in rheumatoid arthritis. Annals Rheumatic Diseases 59, 72–74. https://doi.org/10.1136/ard.59.suppl_1.i72 PMCid:PMC1766621
Gupta M, Sasmal S, Mukherjee A. 2014. Therapeutic Effects of Acetone Extract of Saraca asoca Seeds on Rats with Adjuvant-Induced Arthritis via Attenuating Inflammatory Responses. ISRN Rheumatology. https://doi.org/10.1155/2014/959687 PMid:24729890 PMCid:PMC3960775
Hart DJ, Scott KJ.1995. Development and evaluation of an HPLC method for the analysis of carotenoids in foods, and the measurement of the carotenoid content of vegetables and fruits commonly consumed in the UK. Food Chem. 54, 101–111. https://doi.org/10.1016/0308-8146(95)92669-B
Hemshekhar M, Thushara RM, Jnaneshwari S, Devaraja S, Kemparaju K, Girish KS. 2013. Attenuation of adjuvant-induced arthritis by dietary sesamol via modulation of inflammatory mediators, extracellular matrix degrading enzymes and antioxidant status. European J. Nutrit. 52, 1787–1799. https://doi.org/10.1007/s00394-012-0482-6 PMid:23269651
Kaufmann J, Kielstein V, Kilian S, Stein G, Hein G. 2003. Relation between body mass index and radiological progression in patients with rheumatoid arthritis. J. Rheumatol. 30, 2350–2355 PMid:14677176
Kawase A, Norikane S, Okada A, Adachi M, Kato Y, Iwaki M. 2014. Distinct alterations in ATP-binding cassette transporter expression in liver, kidney, small intestine, and brain in adjuvant-induced arthritic rats. J. Pharmaceutical Sci. 103, 2556–2564. https://doi.org/10.1002/jps.24043 PMid:24912442
Kelmer-Bracht AM, Santos CP, Ishii-Iwamoto EL, Broetto- Biazon AC, Bracht A. 2003. Kinetic properties of the glucose 6-phosphatase of the liver from arthritic rats. Biochim. Biophys. Acta 20, 50–56. https://doi.org/10.1016/S0925-4439(03)00041-3
Knight JA. 2000. Review: Free radicals, antioxidants, and the immune system. Annals Clinical Lab. Sci. 30, 145–158. PMid:10807157
Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. 2001. Method for the measurement of antioxidant activity in human fluids. J. Clinic. Pathol. 54, 356–361. https://doi.org/10.1136/jcp.54.5.356 PMid:11328833 PMCid:PMC1731414
Montserrat-de la Paz S, García-Giménez MD, Ángel-Martín M, Marín-Aguilar F, Fernández-Arche A. 2013. Dietary supplementation evening primrose oil improve symptoms of fibromyalgia syndrome. J. Functional Foods 5, 1279–1287. https://doi.org/10.1016/j.jff.2013.04.012
Perandones CE, Illera VA, Peckham D, Stunz LL, Ashman RF. 1993. Regulation of apoptosis in vitro in mature murine spleen T cells. J. Immunol. (Baltimore, Md :1950) 1, 3521–3529.
Prakash D, Gupta KR. 2009. Antioxidant phytochemicals of Nutraceutical Importance. Open Nutraceutic. J. 2, 20–35.
Rauscher R, Edenharder R, Platt KL. 1998. In vitro antimutagenic and in vivo anticlastogenic effects of carotenoids and solvent extracts from fruits and vegetables rich in carotenoids. Mutation Res. 16, 129–142. https://doi.org/10.1016/S1383-5718(98)00017-5
Rosillo MA, Sánchez?Hidalgo M, Sánchez?Fidalgo S, Aparicio?Soto M, Villegas I, Alarcon?de?la?Lastra C. 2016. Dietary extra?virgin olive oil prevents inflammatory response and cartilage matrix degradation in murine collagen?induced arthritis. Eur. J. Nutr. 55, 315–325. https://doi.org/10.1007/s00394-015-0850-0 PMid:25665892
Satoh K. 1978. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin. Chim. Acta 15, 37–43.
Singh GB, Singh S, Bani S, Gupta BD, Banerjee SK. 1992. Anti-inflammatory activity of oleanolic acid in rats and mice. J. Pharmacy Pharmacol. 44, 456–458. https://doi.org/10.1111/j.2042-7158.1992.tb03646.x PMid:1359067
Singleton VL, Rossi JA. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 16, 144–158.
Sonis ST. 2002. The biologic role for nuclear factor-kappaB in disease and its potential involvement in mucosal injury associated with anti-neoplastic therapy. Crit. Rev. Oral Biol. M. 13, 380–389. https://doi.org/10.1177/154411130201300502 PMid:12393757
Stepaniak JA, Gould KE, Sun D, Swanborg RH. 1995. A comparative study of experimental autoimmune encephalomyelitis in Lewis and DA rats. J. Immunol. (Baltimore, Md :1950) 1, 2762–2769.
Tsaknis J, Lalas S, Lazos ES. 1997. Characterization of crude and purified pumpkin seed oil. Grasas Aceites 48, 267–272. https://doi.org/10.3989/gya.1997.v48.i5.802
Veronezi CM, Jorge N. 2012. Bioactive compounds in lipid fractions of pumpkin (Cucurbita sp) seeds for use in food. J. Food Sci. 77, 653–657. https://doi.org/10.1111/j.1750-3841.2012.02736.x PMid:22671521
Westergren A. 1921. Studies of the suspension stability of the blood in pulmonary tuberculosis. Acta Medica Scandin. 54, 247–245. https://doi.org/10.1111/j.0954-6820.1921.tb15179.x
Wyllie AH, Kerr JF, Currie AR. 1980. Cell death: the significance of apoptosis. Int. Rev. Cytol. 68, 251–306. https://doi.org/10.1016/S0074-7696(08)62312-8
Wyrobek AJ, Bruce WR. in Hallaender A, De Serres FJ (eds.). 1978. The Induction of Sperm-shape Abnormalities in Mice and Humans, in Chemical Mutagens: Principles and Methods for Their Detection 5, 257–285.
Yosida TH, Amano K. 1965. Autosomal polymorphism in laboratory bred and wild Norway rats, Rattus norvegicus, found in Misima. Chromosoma 28, 658–667. https://doi.org/10.1007/BF00285115
Zhang CF, Zhang SL, He X, Yang XL, Wu HT, Lin BQ, Jiang CP, Wang J, Yu CH, Yang ZL, et al., 2014. Antioxidant effects of Genkwa flos flavonoids on Freunds adjuvant-induced rheumatoid arthritis in rats. J. Ethnopharmacol. 14, 793–800. https://doi.org/10.1016/j.jep.2014.03.046 PMid:24685587
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