Characterization of different ozonized sunflower oils II. Triacylglycerol condensation and physical properties




Differential scanning calorimetry, Melting profile, Ozonation, Peroxides, Polar lipids, Sunflower oil, Triacylglycerols


Within the chemical transformations that vegetable oils undergo, oxidative reactions are one of the most widely studied. During the process of oxidation oils react with oxygen in their different forms to produce a large variety of chemical species that alter their chemical and physical properties. This work is the second of a series of two in which the action of ozone on different sunflower oils was investigated. Ozone reacts with double bonds of unsaturated fatty acids which produce different peroxidated species. The presence of altered triacylglycerol (TAG) increases the polarity of the vegetable oils and induces changes in their physical properties. Moreover, peroxygenation alters the aggregation of TAGs, favoring the formation of dimers or oligomers that can substantially change the melting and crystallization profiles of these oils. In the present work the effect of ozonation on four different sunflower oils was studied: common sunflower, high oleic sunflower and two highly saturated sunflower oils, high palmitic and stearic in a high oleic background. Furthermore, the species of TAGs from the different oils that were affected by the ozone oxidative attack were studied. The formation of polar compounds and TAG aggregates were investigated as well as the effect caused by them on the physical properties of the oils as studied through differential scanning calorimetry.


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Harwood JL, Dijkstra AJ, Gunstone FD. 2007. The lipid handbook. CRC Press. Boca Raton, FL. ISBN-13:987-0-8493-9688-5

Waltking AE, H. Wessels. 1981. Chromatographic Separation of Polar and Nonpolar Components of Frying Fats, J. Assoc. Offic. Anal. Chem. 64, 1329-1330. ISSN: 0004-5756.

Choe E, Min DB. 2006. Mechanisms and factors for edible oil oxidation. Comprehensive Rev. Food Sci. Food Safety 5, 169-186.

Min DB, Boff JM. 2002. Lipid oxidation of edible oil. In: Food Lipids. Chemistry, Nutrition and Biotechnology. Marcel Dekker, New York (pp. 335-364). ISBN: 0-8247-0749-4

Márquez-Ruiz G, Pérez-Camino MC, Dobarganes MC. 1990. Combination of adsorption and size-exclusion chromatography for the determination of fatty acid monomers, dimers and polymers. J. Chromatog. A. 514, 37-44.

Sharmin E, Zafar F, Akram D, Alam M, Ahmad S. 2015. Recent advances in vegetable oils based environment friendly coatings: A review. Ind. Crops Prod. 76, 215-229.

Gertz C. 2000. Chemical and physical parameters as quality indicators of used frying fats. Eur. J. Lipid Sci. Technol. 102, 566-572.<566::AID-EJLT566>3.3.CO;2-2

Bailey PS. 1978. Ozonation in Organic Chemistry, Volume I, Olefinic Compounds. Academic Press. New York. ISBN0120731010, 9780120731015

Díaz MF, Gavín-Sazatornil JA, Ledea O, Hernández F, Alaiz M, Garcés R. 2005. Spectroscopic characterization of ozonated sunflower oil. Ozone Sci. Eng. 27, 247-253.

Martínez-Téllez G, Ledea-Lozano O, Díaz-Gómez MF. 2006. Measurement of peroxidic species in ozonized sunflower oil. Ozone Sci. Eng. 28, 181-185.

Carelli AA, Cert A. 1993. Comparative study of the determination of triacylglycerol in vegetable oils using chromatographic techniques. J. Chromatogr. A, 630, 213-222.

Fernández-Moya V, Martínez-Force E, Garcés R. 2005. Oils from improved high stearic acid sunflower seeds. J. Agric. Food Chem. 53, 5326-5330. PMid:15969513

Fernández-Martínez JM, Mancha M, Osorio J, Garcés R. 1997. Sunflower mutant containing high levels of palmitic acid in high oleic background. Euphytica 97, 113-116.

Garcés R, García JM, Mancha M. 1989. Lipid characterization in seeds of a high oleic acid sunflower mutant. Phytochem. 28, 2597-2600.

Li S, Bouzidi L, Narine SS. 2013. Synthesis and physical properties of triacylglycerol oligomers: examining the physical functionality potential of self-metathesized highly unsaturated vegetable oils. Ind. Eng. Chem. Res. 52, 2209-2219.

Criegee R. 1975. Mechanism of ozonolysis. Angewandte Chemie Int. Ed. Eng. 14, 745-752.

Díaz M, Gavín J, Ledea-Lozano OE, Hernández F, Alaiz M, Garcés R. 2005. Spectroscopic Characterization of Ozonated Sunflower Oil. Ozone Sci. Eng. 27, 247-253.

Bailey PS, Ferrell TM, Rustaiyan A, Seyhan S, Unruh LE. 1978. Stereochemistry of ozonide formation. Effects of complexing agents and rate of warm-up. J. Am. Chem. Soc. 100, 894-898.



How to Cite

Ledea-Lozano OE, Fernández-García LA, Gil-Ibarra D, Bootello M Á., Garcés R, Martínez-Force E, Salas JJ. Characterization of different ozonized sunflower oils II. Triacylglycerol condensation and physical properties. grasasaceites [Internet]. 2019Dec.30 [cited 2022Dec.4];70(4):e330. Available from:




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