Antioxidant activity of 100% and 80% methanol extracts from barley seeds (Hordeum vulgare L.): stabilization of sunflower oil


  • Farooq Anwar Department of Chemistry and Biochemistry, University of Agriculture
  • Hafiz Muhammad Abdul Qayyum Department of Chemistry and Biochemistry, University of Agriculture
  • Abdullah Ijaz Hussain Department of Chemistry and Biochemistry, University of Agriculture - Department of Chemistry, Government College University
  • Shahid Iqbal Department of Chemistry, University of Sargodha



Accelerated storage, Antioxidants, DPPH scavenging, Hordeum vulgare L., Oxidation products, Phenolics, Stabilization, Sunflower oil.


The antioxidant potential of 100% and 80% methanol extracts from the seeds of three barley varieties (Jou 83, Jou 87 and Haider 93) was assessed. The extract yields from barley seeds ranged from 3.23% (Haider 93,100% methanol) to 5.31% (Jou 83, 80% methanol). The total phenolic contents, DPPH radical scavenging activity (IC50 values) and inhibition of linoleic acid oxidation of barley seed extracts (BSE) were determined to be 88.1-145.7 mg/100g, 90.8-168.6 μg/mL and 62.6-74.6%, respectively. The antioxidant effectiveness of BSE was also assessed by stabilizing sunflower oil (SFO) with BSE at a concentration of 600 ppm (oil weight basis). The stabilized (treated with extract) and the control (without extract addition) SFO samples were subjected to accelerated (oven heating at 60ºC for 30 days, 8 h heating cycle/day) storage. These were analyzed at regular intervals for the extent of oxidative changes according to the measurements of their contents of peroxide value, para-anisidine value, conjugated dienes and conjugated trienes. Generally, the 80% methanol extract of barely seeds demonstrated better antioxidant action than the 100% methanol extract. The antioxidant activity of BSE was also found to be considerably varied among the varieties tested. The present results suggest that antioxidant extracts from barely seeds might be used to protect vegetable oils from oxidation.


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American Oil Chemists Society (AOCS). 1997. Official Methods and Recommended Practices of the AOCS, (5th ed.), AOCS Press, Champaign.

Anwar F, Jamil A, Iqbal S, Sheikh MA. 2006. Antioxidant activity of various plant extracts under ambient and accelerated storage of sunflower oil. Grasas y Aceites 57, 189-197. doi:10.3989/gya.2006.v57.i2.36

Arabshahi-Delouee S, Urooj A. 2007. Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chem. 102, 1233-1240. doi:10.1016/j.foodchem.2006.07.013

Bonolie M, Verardo V, Marconi E, Caboni MF. 2004. Antioxidant phenols in barely (Hordeum vulgare L.) flour: comparative spectrophotometric study among extraction methods of free and bound phenolic compound. J. Agri. Food Chem. 52, 5195-5200. doi:10.1021/jf040075c PMid:15291496

Bozin B, Mimica-Dukie N, Simin N, Anackov G. 2006. Characterization of the volatile composition of essential oil of some lamiaceae species and the antimicrobial and antioxidant activities of the entire oils. J Agri. Food Chem. 54, 1822-1828. doi:10.1021/jf051922u PMid:16506839

Chaovanalikit A, Wrolstad RE. 2004. Total anthocyanins and total phenolics of fresh and processed cherries and their antioxidant properties. J. Food Sci. 69, 67-72. doi:10.1111/j.1365-2621.2004.tb17858.x

Chatha SAS, Anwar F, Manzoor M, Bajwa JR. 2006. Evaluation of the antioxidant activity of rice bran extracts using different antioxidant assays. Grasas y Aceites 57, 328-335.

Choi Y, Ku JB, Chang HB, Lee J. 2007. Antioxidant activities and total phenolics of ethanol extracts from several edible mushrooms produced in Korea. Food Sci. Biotech. 14, 703-770.

Ghiselli A, Serafini M, Natella F, Scaccini C. 2000. Total antioxidant capacity as a tool to assess redox status: critical view and experimental data. Free Rad. Bio. Medi. 29, 1106-1114. doi:10.1016/S0891-5849(00)00394-4

Hussain AI, Anwar F, Sherazi STH, Przybylski R. 2008. Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chem. 108, 986-995. doi:10.1016/j.foodchem.2007.12.010

International Union of Pure and Applied Chemistry (IUPAC). 1987. Standard Methods for the Analysis of Oils, Fats and Derivatives, Paquot C and Hautfenne A (Eds.), 7th Revised Version, Blackwell Scientific, London.

Iqbal S, Bhanger MI, Anwar F. 2005. Antioxidant properties and components of some commercially available varieties of rice bran in Pakistan. Food Chem 93, 265-272. doi:10.1016/j.foodchem.2004.09.024

John AM, Grohmann K. 2001. Phenols in citrus peel by products, Concentration of hydroxycinnamates and polymethoxylated flavones in citrus peel molasses. J. Agri. Food Chem. 49, 3268-3273. doi:10.1021/jf010011r PMid:11453761

Juntunen KS, Mazur WM, Liukkonen KH, Uehara M, Poutanen KS, Adlercreutz HCT, et al. 2000. Consumption of whole meal rye bread increases serum concentrations and urinary excretion of enterolactone compared with consumption of white wheat bread in healthy Finnish men and women. British J. Nut. 84, 839-846. PMid:11177200

Karppinen S, Myllymaki O, Forssell P, Poutanen K. 2003. Fructan content of rye and rye products. Cereal Chem. 80, 168-171. doi:10.1094/CCHEM.2003.80.2.168

Li Y, Guo C, Yang J, Wei J, XU J, Cheng S. 2006. Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chem. 96, 254-260. doi:10.1016/j.foodchem.2005.02.033

Liu Q, Yao H. 2007. Antioxidant activities of barley seeds extracts. Food Chem. 102, 732-737. Madhujith T, Izydorczyk M, Shahidi F. 2006. Antioxidant potential of pearled barley (Hordium species). J. Agric. Food Chem. 54, 283-3289.

Madhujith T, Izydorczyk M, Shahidi F. 2006. Optimization of the extraction of antioxidative constituents of six barley cultivars and their antioxidant properties. J. Agric. Food Chem. 54, 8048-805. doi:10.1021/jf061558e PMid:17032008

Madhujith T, Naczk M, Shahidi F. 2004. Antioxidant activity of common beans (pha seolus vulgaris L.) J. Food Lipids. 11, 220-33. doi:10.1111/j.1745-4522.2004.01134.x

Madhujith T, Shahidi F. 2008. Antioxidant and antiproliferative potential of pearled barley (Hordeun vulgare L). Pharm. Bio. 46, 88-95. doi:10.1080/13880200701734752

Madhujith T, Shahidi F. 2009. Antioxidant potential of barley as affected by alkaline hydrolysis and release of insoluble-bound phenolics. Food Chem. 117, 615-620. doi:10.1016/j.foodchem.2009.04.055

Mcginely, L. 1991. Analysis and quality control for processing and processed fats. In: Analysis of Oilseeds, Fats and Fatty Foods, Rossell JB and Pritchard JLR (Eds.), New York, Elsevier Applied Science, pp. 460–470.

Peschel W, Sanchez-Rabaneda F, Dn W, Plescher A, Gartzia I, Jimenez D, et al. 2006. An industrial approach in the search of natural antioxidants from vegetable and fruit wastes. Food Chem 97, 137-150. doi:10.1016/j.foodchem.2005.03.033

Shahidi F, Wanasundara UN. 1997. Measurement of lipid oxidation and evaluation of antioxidant activity, In: Natural Antioxidants, Chemistry, Health Effects and Applications, Shahidi F (Ed.), AOCS Press Champaign IL, USA.

Shahidi F. 1997. Natural antioxidants: an overview, In: Natural Antioxidants, Chemistry, Health Effects and Applications, Shahidi F (Ed.), AOCS Press Champaign, Illinois, USA, pp.1-10.

Siddiq A, Anwar F, Manzoor M, Fatima M. 2005. Antioxidant activity of different solvent extracts of Moringa oleifera leaves under accelerated storage conditions of sunflower oil. Asian J. Plant Sci. 4, 630-635. doi:10.3923/ajps.2005.630.635

Sultana B, Anwar F, Asi MR, Chatha SAS. 2008. Antioxidant potential of extracts from different agro wastes: Stablization of corn oil. Grasas y Aceites 59, 205-217.

Velioglu mazza SG, Gao L, Oomah BD. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J. Agric. Food Chem. 46, 4113-4117.

Wyen DV, Takacsova M, Jakubik T, Dang M. 2000. Antioxidant effects of thyme in rape seed oil. Biologia Bratislava 55, 277-281.

Zeilinski H, Kozlowoka H. 2000. Antioxidant activity and total phenolics in selected cereal grains and their different morphological fractions. J. Agri. Food Chem. 48, 2008-2016. doi:10.1021/jf990619o

Zhao H, Fan W, Dong J, Lu J, Chen J, Shan L, Lin Y, Kong W. 2008. Evaluation of antioxidant activities and total phenolic contents of typical malting barley varieties. Food Chem. 107, 296-304. doi:10.1016/j.foodchem.2007.08.018




How to Cite

Anwar F, Abdul Qayyum HM, Ijaz Hussain A, Iqbal S. Antioxidant activity of 100% and 80% methanol extracts from barley seeds (Hordeum vulgare L.): stabilization of sunflower oil. grasasaceites [Internet]. 2010Sep.30 [cited 2023Jan.30];61(3):237-43. Available from:




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