Antioxidant capacity of the phenolic fraction and its effect on the oxidative stability of olive oil varieties grown in the southwest of Spain

Authors

  • M. N. Franco Technological Agri-Food Institute (INTAEX)
  • T. Galeano-Díaz bAnalytical Chemistry Department. University of Extremadura
  • J. Sánchez Technological Agri-Food Institute (INTAEX)
  • C. De Miguel Vegetal Biology, Ecology and Soil Sciences Department. University of Extremadura
  • D. Martín-Vertedor Technological Agri-Food Institute (INTAEX)

DOI:

https://doi.org/10.3989/gya.051513

Keywords:

ABTS radical cation, Antioxidant capacity, Olive oil, Oxidative stability, Phenolics

Abstract


The characterization of olive oils from seven representative fruit varieties (Arbequina, Carrasqueña, Corniche, Manzanilla Cacereña, Morisca, Picual, and Verdial de Badajoz) from the southwest of Spain is carried out according to antioxidant capacity of the phenolic fraction and oxidative stability in different ripening stages. Antioxidant capacity is measured through the reduction of a 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid radical cation previously oxidized with peroxidase/hydrogen peroxide. The decrease in absorbance at 730 nm at 3 min was measured. Values like Trolox Equivalents Antioxidant Capacity and oxidative stability varied from 0.6 to 2.5 mmol Trolox·kg–1 oil and 28.3 to 170.9 hours Rancimat respectively. The best positive correlation between total phenolic compounds and antioxidant capacity were in the Carrasqueña and Arbequina varieties. The rest showed moderated correlations. Correlation between antioxidant capacity and oxidative stability was found in a range from 0.66 to 0.97, depending on varieties.

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References

Agati G, Pinelli P, Cortés S, Romani A, Cartelat A, Cerovic ZG. 2005. Nondestructive evaluation of anthocyanins in olive Olea europaea fruits by in situ chlorophyll fluorescente spectroscopy. J. Agric. Food. Chem. 53, 1354–1363. http://dx.doi.org/10.1021/jf048381d

Aguilera MP, Beltrán G, Ortega D, Fernández A, Jiménez A, Uceda M. 2005. Characterisation of virgin olive oil of Italian olive cultivars: "Frantoio" and "Leccino", grown in Andalusia. Food Chem. 89, 387–391. http://dx.doi.org/10.1016/j.foodchem.2004.02.046

Arnao MB. 2000. Some methodological problems in the determination of antioxidant activity using chromogen radicals: a pratical case. Trends Food Sci. Techol. 11, 419–421. http://dx.doi.org/10.1016/S0924-2244(01)00027-9

Arslan D, Schreiner M. 2012. Chemical characteristics and antioxidant activity of olive oils from Turkish varieties grown in Hatay province. Scientia Hortic. 144, 141–152. http://dx.doi.org/10.1016/j.scienta.2012.07.006

Baccouri O, Guerfel M, Baccouri B, Cerretani L, Bendini A, Lercker G, Zarrouk M, Daoud D, Miled B. 2008. Chemical composition and oxidative stability of Tunisian monovarietal virgin olive oils with regard to fruit ripening. Food Chem. 109, 743–754. http://dx.doi.org/10.1016/j.foodchem.2008.01.034

Baiano A, Gambacorta G, Terracone C, Previtali MA, La Notte E. 2009. Characteristics of drupes, phenolic content and antioxidant capacity of Italian olive fruits. J. Food Lipids 16, 209–226. http://dx.doi.org/10.1111/j.1745-4522.2009.01142.x

Baldioli M, Servili M, Perretti G, Montedoro GF. 1996. Antioxidant activity of tocopherols and phenolic compounds of virgin olive oil. J. Am. Oil Chem. Soc. 73, 1589–1593. http://dx.doi.org/10.1007/BF02523530

Beltrán G, Jiménez A, Aguilera MP, Uceda M. 2000. Análisis mediante HPLC de la fracción fenólica del aceite de oliva virgen de la variedad Arbequina. Relación con la medida de amargor K225 y la estabilidad. Grasas Aceites 215, 320–324.

Bendini A., Cerretani L., Carrasco-Pancorbo A., Gomez-Caravaca A.M., Segura-Carretero A., Fernandez-Gutierrez A. 2007. Phenolic molecules in virgin olive oils: A survey of their sensory properties, health effects, antioxidant activity and analytical methods. An over-view of the last decade. Molecules 12, 1679–1719. http://dx.doi.org/10.3390/12081679

Calvo P, Hernández MT, Lozano M, González D. 2010. Microencapsulation of extra-virgin olive oil by spray-drying: Influence of wall material and olive quality. Eur. J. Lipid Sci. Technol. 112, 852–858. http://dx.doi.org/10.1002/ejlt.201000059

Calvo P, Casta-o AL, Lozano M, González D. 2012. Influence of the microencapsulation on the quality parameters and shelf-life of extra-virgin olive oil encapsulated in the presence of BHT and different capsule wall components. Food Res. Int. 45, 256–261. http://dx.doi.org/10.1016/j.foodres.2011.10.036

Cano A, Hernández-Ruiz J, García-Cánovas F, Acosta M, Arnao MB. 1998. An end-point method for estimation of the total antioxidant activity in plants material. Phytochem Anal. 9, 196–202. http://dx.doi.org/10.1002/(SICI)1099-1565(199807/08)9:4<196::AID-PCA395>3.0.CO;2-W

Cano A, Acosta M, Arnao MB. 2000. A method to measure antioxidant activity in organic media: application to lipophilic vitamins. Redox Rep. 5, 365–70. http://dx.doi.org/10.1179/135100000101535933

Cert A, Alba J, Pérez-Camino MC, Ruiz-Gómez A, Hidalgo F, Moreda W, Moyano MJ, Martínez F, Tubaileh R, Olías JM. 1999. "Influencia de los sistemas de extracción sobre las características y los componentes menores del aceite de oliva virgen extra". Ciencia y Técnica 79, 41–50.

Del Carlo M, Saccheti G, Di Mattia C, Compagnone D, Mastrocola D, Liberatore L, Cichelli A. 2004. Contribution of the phenolic fraction to the antioxidant activity and oxidative stabilite of olive oils. J. Agric. Food. Chem. 52, 4072–4079. http://dx.doi.org/10.1021/jf049806z

Clayton CA, Hines JW, Elkins PD. 1987. Detection limits with specified assurance probabilities. Anal. Chem. 59, 2506–2514. http://dx.doi.org/10.1021/ac00147a014

Dugo G, Lo Turco V, Pollicino D, Mavrogeni E, Pipitone F. 2004. Caracterización de los aceites de oliva vírgenes sicilianos. Variaciones cualitativas en los aceites de las variedades "Biancolilla", "Nocellara del Belice", "Cerasuola", "Tonda Iblea", y Crastu" dependiendo de las técnicas y el periodo de recolección de las aceitunas. Olivae 101, 44–51.

Esti, M., Contini, M., Moneta, E., Sinesio, F. 2009. Phenolic compounds and temporal perception of bitterness and pungency in extra virgin olive oils: changes occurring throughout storage. Food Chem. 113, 1095–1100. http://dx.doi.org/10.1016/j.foodchem.2008.08.076

Fernández-Pachón MS, Villano D, Troncoso AM, García-Parrilla MC. 2005. Antioxidant capacity of plasma after red wine intake in human volunteers. J. Agric. Food. Chem. 53, 5024–29. http://dx.doi.org/10.1021/jf0501995

Fuentes M, Marín J, De Miguel C, Sánchez J. 2008. Extraction Systems and quality parameters of extra virgin olive oils from the olive-oil producing zone "Tierra de Barros" Extremadura, Spain. Riv. Ital. Sostanze Grasse 85, 48–55.

Gorinstein S, Yamamoto K, Katrich E, Leontowicz H, Lojek A, Leontowicz M, Ciz M, Goshev I, Shalev U, Trakhtenberg S. 2003. Antioxidative properties of Jaffa sweeties and grapefruit and their influence on lipid metabolism and plasma antioxidative potential in rats. Biosci. Biotechnol. Biochem. 67, 907–910. http://dx.doi.org/10.1271/bbb.67.907

Gutiérrez F. 1989. Determinación de la estabilidad oxidativa de aceites de oliva vírgenes: comparación entre el método del oxígeno activo A.O.M y el método Rancimat. Grasas Aceites 401, 1–5.

Gutiérrez F, Jiménez B, Ruíz A, Albi A. 1999. Effect of olive ripeness on the oxidative stability of virgin olive oil extraxted from the varieties picual and hojiblanca and on different components involved. J. Agric. Food. Chem. 47, 121–127. http://dx.doi.org/10.1021/jf980684i

Holasova M, Fiedlerova V, Smrcinova H, Orsak M, Lachman J, Vavreinova S. 2002. Buckwheat – The source of antioxidant activity in functional foods. Food Res. Int. 35, 207–211. http://dx.doi.org/10.1016/S0963-9969(01)00185-5

Kedage W, Tilak JC, Dixit GB, Devasagayam TP, Mhatre M. 2007. A study of antioxidant properties of some varieties of grapes Vitis vinifera L. Crit. Rev. Food Sci. Nutr. 472, 175–185. http://dx.doi.org/10.1080/10408390600634598

Long GL, Winefordner JD. 1983. Limits of Detection: A Closer Look at the IUPAC Definition. Anal. Chem. 55, 712–724.

Martínez JM, Mu-oz E, Alba J, Lanzón A. 1975. Informe sobre utilización del Analizador de Rendimientos "Abencor". Grasas Aceites 266, 379–385.

Mateos, R., 2002. Caracterización de componentes fenólicos del aceite de oliva y su relación con la estabilidad oxidativa y el amargo. Tesis Doctoral, Universidad de Sevilla.

Mateos R, Espartero JL, Trujillo M, Rios JJ, León-Camacho M, Alcudia F, Cert A. 2001. Determination of phenols, flavones and lignans in virgin olive oils by solid phase extraction and high-performance liquid chromatography with diode array ultraviolet detection. J. Agric. Food. Chem. 49, 2185–2192. http://dx.doi.org/10.1021/jf0013205

Montedoro G, Servili M, Baldioli M, Miniati E. 1992. Simple and hydrolizable phenolic compounds in virgin olive oil. 1. Their extraction, separation and quantitative and semiquantitative evaluation by HPLC. J. Agric. Food. Chem. 40, 1571–1576. http://dx.doi.org/10.1021/jf00021a019

Morelló JR, Vuorela S, Romero MP, Motilva MJ, Heinonen M. 2005. Antioxidant activity of olive pulp and olive oil phenolic compounds of the Arbequina cultivar. J. Agric. Food. Chem. 53, 2002–2008. http://dx.doi.org/10.1021/jf048386a

Oueslati I, Anniva C, Daoud D, Tsimidou MZ, Zarrouk M. 2009. Virgin olive oil VOO production in Tunisia: The commercial potential of the major olive varieties from the arid Tataouine zone. Food Chem. 112, 733–741. http://dx.doi.org/10.1016/j.foodchem.2008.06.041

Paiva-Martins F, Fernandes J, Santos V, Silva L, Borges F, Rocha S. 2010. Powerful protective role of 3,4-dihydroxyphenylethanol-elenolic acid dialdehyde against erythrocyte oxidative-induced hemolysis. J. Agric. Food. Chem. 58, 135–140. http://dx.doi.org/10.1021/jf9031052

Pérez-Arquillué C, Juan T, Valero N, Estopa-án G, Ari-o A, Conchello P, Herrera A. 2003. Estudio de la calidad del aceite de oliva virgen de Aragón. Grasas Aceites 54, 151–160.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidants activity applying an improved ABTS radical catión decolorization assay. Free Radical Biol. Med. 26, 1231–7. http://dx.doi.org/10.1016/S0891-5849(98)00315-3

Salvador MD, Aranda F, Fregapane G. 2001. Influence of fruit ripening on "Cornicabra" virgin olive oil quality A study of four successive crop seasons. Food Chem. 73, 45–53. http://dx.doi.org/10.1016/S0308-8146(00)00276-4

Samaniego C, Troncoso AM, García MC, Quesada JJ, López H, López MC. 2007. Different radical scavenging tests in virgin olive oil and their relation to the total phenol content. Anal. Chem. Acta 593, 103–107. http://dx.doi.org/10.1016/j.aca.2007.04.037

Sellappan S, Akoh CC, Krewer G. 2002. Phenolic compounds and antioxidant capacity of Georgia-Grown blueberries and blackberries. J. Agric. Food. Chem. 50, 2432–2438. http://dx.doi.org/10.1021/jf011097r

Tovar MJ, Romero MP, Alegre S, Girona J, Motilva MJ. 2003. Composition and organoleptic characteristics of oil from Arbequina olive Olea europaea L. trees under deficit irrigation. J. Sci. Food Agric. 82, 1755–1763. http://dx.doi.org/10.1002/jsfa.1246

Ubando-Rivera J, Navarro-Oca-a A, Valdivia-López MA. 2005. Mexican lime peel: Comparative study on contents of dietary fibre and associated antioxidant activity. Food Chem. 89, 57–61. http://dx.doi.org/10.1016/j.foodchem.2004.01.076

Uceda M, Frías L. 1975. Época de recolección. Evolución del contenido graso y de la composición y la calidad del aceite. Proceeding II Seminario Oleícola Internacional, Córdoba.

Uceda M, Hermoso M. 2001. La calidad del aceite de oliva, in El cultivo del olivo. Barranco D, Fernández-Escobar R and Rallo L (Eds.) MundiPrensa, Madrid, 589–614.

Valavanidis A, Nisiotou C, Papageorgiou Y, Kremli I, Satravelas N, Zinieris N, Zygalaki H. 2004. Comparison of the radical scavenging potential of polar and lipidic fractions of olive oil and other vegetable oils under normal conditions and after thermal treatment. J. Agric. Food. Chem. 52, 2358–2365. http://dx.doi.org/10.1021/jf030491h

Velioglu YS, Mazza G, Gao L, Oomah BD. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J. Agric. Food. Chem. 46, 4113–4117. http://dx.doi.org/10.1021/jf9801973

Villaño D, Fernández-Pachón MS, Troncoso AM, García-Parrilla MC. 2006. Influence of enological practices on the antioxidant activity of wines. Food Chem. 95, 394–404. http://dx.doi.org/10.1016/j.foodchem.2005.01.005

Published

2014-03-30

How to Cite

1.
Franco MN, Galeano-Díaz T, Sánchez J, De Miguel C, Martín-Vertedor D. Antioxidant capacity of the phenolic fraction and its effect on the oxidative stability of olive oil varieties grown in the southwest of Spain. Grasas aceites [Internet]. 2014Mar.30 [cited 2024Apr.20];65(1):e004. Available from: https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1469

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