Changes in proximate composition and oil characteristics during flaxseed development

W. Herchi; S. Bahashwan; H. Trabelsi; S. Boukhchina; H. Kallel; S. Rochut; C. Pepe

doi:10.3989/gya.097713

Autores/as

W. Herchi Laboratoire de Biochimie des Lipides, Département de Biologie, Faculté des Sciences de Tunis
S. Bahashwan College of Pharmacy, Taibah University
H. Trabelsi Laboratoire de Biochimie des Lipides, Département de Biologie, Faculté des Sciences de Tunis
S. Boukhchina Laboratoire de Biochimie des Lipides, Département de Biologie, Faculté des Sciences de Tunis
H. Kallel Laboratoire de Biochimie des Lipides, Département de Biologie, Faculté des Sciences de Tunis
S. Rochut UPMC University Paris
C. Pepe UPMC University Paris

DOI:

https://doi.org/10.3989/gya.097713

Palabras clave:

APPI-MS, Composición proximal, Fracciones de proteínas, HPLC, Madurez, Semillas de linaza

Resumen

Fotoionización a presión atmosférica-Espectrometría de masas (APPI-MS) y cromatografía líquida de alta resolución (HPLC) son dos métodos de análisis que se utilizaron para caracterizar triglicéridos (TAGs) durante el desarrollo de semillas de linaza. El método HPLC mostró la presencia de 15 especies de TAG, en contraste, los cromatogramas de APPI-MS mostraron 17 picos de TAG siendo el método APPI-MS más rápido que el de HPLC (11 min). El índice de yodo de los aceites mostró un aumento gradual, mientras que la estabilidad disminuyó continuamente. El estudio de la composición proximal de la linaza durante su desarrollo, mostró que esta semilla es una fuente potencialmente buena de energía y de proteína para la dieta. Al final de la maduración, la linaza contenía 37 % de aceite y 24 % de proteína sobre peso seco; albúmina fue la principal proteína de almacenamiento (53% sobre el total de las proteínas de almacenamiento) seguido de la globulina (33 %) y glutelina (11 %). Las prolaminas presentaron el porcentaje más bajo con 3 %. La actividad α-amilasa fue mayor en las semillas maduras que en las más jóvenes.

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Aguilera MC, Ramirez-Tortosa MC, Mesa MD, Gil A. 2000. Do MUFA and PUFA have beneficial effects on development of cardiovascular disease? In: Pandai SG (Eds) Recent research developments in lipids. Adv. Lipid Res. 1, 369–390.

American Oil Chemists Society (AOCS). 1993. Official Methods and Recommended Practices of the American Oil Chemists' Society, 4th edn., edited by D. Firestone, AOCS Press, Champaign, IL.

Association of Official Analytical Chemists (AOAC). 1984. Official Methods of the Association of Official Analytical Chemists, fourteenth ed. AOAC, Arlington, Method 28.110.

Association of Official Analytical Chemists (AOAC). 1990. Official methods of analysis, 15th edn. Association of Official Analytical Chemists, Washington, DC.

Association of Official Analytical Chemists (AOAC). 2005. In: Firestone D (Ed) Official methods of analysis of the Association of the Official Analytical Chemists. Assoc Off Anal Chem Inc, VA.

Attoumbré J, Boubé A, Laoualy M, Bienaimé C, Dubois F, Baltora-Rosset S. 2011. Investigation of lignan accumulation in developing Linum usitatissimum seeds by immunolocalization and HPLC. Phytochem. Lett. 4, 194–198. http://dx.doi.org/10.1016/j.phytol.2011.03.004

Baccouri B, Zarrouk W, Krichene D, Nouairi I, Ben Youssef N, Daoud D, Zarrouk M. 2007. Influence of Fruit Ripening and Crop Yield on Chemical Properties of Virgin Olive Oils from Seven Selected Oleasters (Olea europea L.). J. Agron. 6, 388–396. http://dx.doi.org/10.3923/ja.2007.388.396

Barrett MA, Larkin PJ. 1977. Milk and beef production in the tropics. London: ELBS and Oxford University Press.

Bewley JD, Black N. 1978. In Physiology and biochemistry of seeds in relation to germination. New York: Springer Verlag. 1, 306–307.

Bligh EG. Dyer WJ. 1959. A rapid method of total lipid extraction and purification. Can J Biochem. Physiol. 37, 911–917. http://dx.doi.org/10.1139/o59-099 PMid:13671378

Chavan UD, Mckenzie DB, Shahidi F. 2001. Protein classification of beach pea (Lathyrus maritimus L.). Food Chem. 75, 145–153. http://dx.doi.org/10.1016/S0308-8146(01)00122-4

Chen Z, Ilarslan H, Palmer RG, Shoemaker RC.1998. Development of protein bodies and accumulation of carbohydrates in a soybean (Leguminosae) shrivelled seed mutant. Am. J. Bot. 85, 492–499. http://dx.doi.org/10.2307/2446432 PMid:21684931

Douce R. 1964. Identification et dosage de quelques glycérophosphosphatides dans des souches normales et tumorales de scosonères cultivés in vitro. CR Acad. Sci. 259, 3066–3068.

Epaminondas PS, Araujo KLGV, Lima de Souza A, Silva MCD, Queiroz N, Souza AL, Soledade LEB, Santos IMG, Souza AG. 2011. Influence of toasting on the nutritious and thermal properties of flaxseed. J. Therm. Anal. Calorim. 106, 551–555. http://dx.doi.org/10.1007/s10973-011-1638-y

Folch J, Lees M, Sloane Stanley GMA. 1957. Simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497–509. PMid:13428781

Gutiérrez F. 1989. Determination of virgin olive oils stability: Comparization between activated oxygen (AOM) and Rancimat Methods. Grasas Aceites. 40, 1–5.

Herchi W, Harrabi S, Sebei K, Rochut S, Boukhchina S, Pepe C, Kallel H. 2009. Phytosterols accumulation in the seeds of Linum usitatissimum L. Plant Physiol. Biochem. 47, 880–885. http://dx.doi.org/10.1016/j.plaphy.2009.07.001 PMid:19616960

Herchi W, Sakouhi F, Arraez-Roman D, Segura-Carretero A, Boukhchina S, Kallel H, Fernández-Gutiérrez A. 2011. Changes in the Content of Phenolic Compounds in Flaxseed Oil during Development. J. Am. Oil Chem. Soc. 88, 1135–1142. http://dx.doi.org/10.1007/s11746-011-1783-2

Herchi W, Trabelsi H, Ben Salah H, Yuan Zhao Y, Boukhchina S, Kallel H, Curtis JM. 2012. Changes in the triacylglycerol content of flaxseeds during development using liquid chromatography-atmospheric pressure photoionizationmass spectrometry (LC-APPI-MS). Afr. J. Biotechnol. 11, 904–911.

Hosseinian FS, Muir AD, Westcott ND, Krol ES. 2006. Antioxidant capacity of flaxseed lignans in two model systems. J. Am. Oil Chem. Soc. 83, 835–840. http://dx.doi.org/10.1007/s11746-006-5034-x

Hunt C, Burn P, Adamczuk ZC. 1987. Proximate composition and fat type of three popular take-away meals. Int. J. Food Sci. Technol. 22, 669–675. http://dx.doi.org/10.1111/j.1365-2621.1987.tb00535.x

Kaur M, Singh N. 2007. Characterization of protein isolates from different Indian chickpea (Cicer arietinum L.) cultivars. Food Chem. 102, 366–374. http://dx.doi.org/10.1016/j.foodchem.2006.05.029

Kohno A, Nanmori T. 1991. Changes in α-and β-amylase activities during germination of seeds of alfalfa. Plant Cell Physiol. 32, 459–466.

Lee YI, Yeung EC, Lee N, Chung MC. 2008. Embryology of Phalaenopsis amabilis var. Formosa: embryo development. Bot Stud. 49, 139–146.

Lei B, Li Chen. Oomah BD, Mazza G. 2003. Distribution of cadmium-binding components in flax (Linum usitatissimum L.) seed. J Agric Food Chem. 51, 814–821. http://dx.doi.org/10.1021/jf0209084 PMid:12537463

Mangold HK. 1964. Thin layer chromatography of lipids. J. Am. Oil Chem. Soc. 47, 762–773. http://dx.doi.org/10.1007/BF02676266

Miquel M, Browse J. 1997. Lipid biosynthesis in developing seeds, in: J. Kigel, G. Galili (Eds.), Seed Development and Germination. Dekker, USA, 169–193.

Monma M, Sugimoto T, Monma M, Kawanura Y, Saio K. 1991. Starch breakdown in developing soybean seeds. Agr. Biol. Chem. 55, 67–71. http://dx.doi.org/10.1271/bbb1961.55.67

Mueller K, Eisner P, Yoshie-Stark Y, Nakada R, Kirchhoff E. 2010. Functional properties and chemical composition of fractionated brown and yellow linseed meal (Linum usitatissimum). J. Food Eng. 98, 453–460. http://dx.doi.org/10.1016/j.jfoodeng.2010.01.028

Murtaza G, Asghar R. 2012. α-amylase activities during seed development and germination in pea (pisum sativum L) treated with salicylic acid. Pak J. Bot. 44, 1823−1829.

Olsen OA. 2001. Endosperm development: cellularization and cell fate specification. Annu Rev Plant Physiol. Plant Mol. Biol. 52, 233–267. http://dx.doi.org/10.1146/annurev.arplant.52.1.233 PMid:11337398

Oomah B. 2001. Flaxseed as a functional food source. J. Sci. Food Agr. 81, 889–894. http://dx.doi.org/10.1002/jsfa.898

Osborne TB. 1924. The vegetable proteins. Longmans, Green and Co., London, 51–56.

Pang PP, Pruitt RE, Meyerowitz EM. 1988. Molecular cloning, genomic organization, expression and evolution of 12S seed storage protein genes of Arabidopsis thaliana. Plant Mol. Biol. 11, 805–820. http://dx.doi.org/10.1007/BF00019521 PMid:24272631

Saldivar X, Wanga YJ, Chen P, Hou A. 2011. Changes in chemical composition during soybean seed development. Food Chem. 124, 1369–1375. http://dx.doi.org/10.1016/j.foodchem.2010.07.091

Wanasundara PKJPD, Shahidi F, Brosnan ME. 1999. Changes in flax (Linum usitatissimum) seed nitrogenous compounds during germination. Food Chem. 65, 289–295. http://dx.doi.org/10.1016/S0308-8146(98)00176-9