Changes in fatty acid profile of Holothuria forskali muscle following acute mercury exposure

I.  Rabeh; K.  Telahigue; T.  Hajji; C.  Fouzai; M.  El Cafsi; N.  Soudani

doi:10.3989/gya.0335201

Authors

I. Rabeh Laboratory of Ecology, Biology and Physiology of Aquatic organisms, Faculty of Sciences of Tunis, Univ. Tunis El Manar https://orcid.org/0000-0002-0307-473X
K. Telahigue Laboratory of Ecology, Biology and Physiology of Aquatic organisms, Faculty of Sciences of Tunis, Univ. Tunis El Manar https://orcid.org/0000-0001-8841-9911
T. Hajji BVBGR-LR11ES31, Higher Institute of Biotechnology - Sidi Thabet, Biotechpole Sidi Thabet, Univ. Manouba https://orcid.org/0000-0002-3869-8876
C. Fouzai Laboratory of Ecology, Biology and Physiology of Aquatic organisms, Faculty of Sciences of Tunis, Univ. Tunis El Manar https://orcid.org/0000-0001-7588-1859
M. El Cafsi Laboratory of Ecology, Biology and Physiology of Aquatic organisms, Faculty of Sciences of Tunis, Univ. Tunis El Manar, https://orcid.org/0000-0002-9771-1110
N. Soudani Laboratory of Ecology, Biology and Physiology of Aquatic organisms, Faculty of Sciences of Tunis, Univ. Tunis El Manar https://orcid.org/0000-0002-7652-9678

DOI:

https://doi.org/10.3989/gya.0335201

Keywords:

Acute exposure, Fatty acid composition, Holothuria forskali, Indices of lipid peroxidation, Mercuric chloride (HgCl2), Sea cucumber

Abstract

The present study aimed to document the interaction between mercury (Hg), as a model chemical stressor to an aquatic organism, and Fatty acid (FA) profile in the longitudinal muscle of the sea cucumber Holothuria forskali. To assess the sensitivity of this species to the toxic effects of Hg, young H. forskali were exposed to gradual doses of Hg (40, 80 and160 µg·L^-1) for 96 h. The results showed that following Hg exposure, the FA profile of H. forskali corresponded to an increase in the level of saturated fatty acids, and the decrease in the level of monounsaturated and polyunsaturated fatty acids. The most prominent changes in the FA composition were recorded at the lowest dose with noticeable decreases in linoleic, arachidonic and eicosapentaenoic acid levels and an increase of docosahexaenoic acid. The occurrence of a state of oxidative stress induced by Hg contamination was evidenced by the enhanced levels of malondialdehyde, hydrogen peroxide and lipid hydroperoxide. Overall, the low concentration of mercury exerted the most obvious effects on lipid metabolism, suggesting that changes in fatty acid composition may be act as an early biomarker to assess mercury toxicity in this ecologically and economically important species.

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